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[176613_at]

Description W09B12.1 /REP_DB=WormBase Gene ID /WP=CE07569 /GEN=ace-1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 07      4grp_highest_8_8 > 60      4grp_high_8_8 > 17      6grp_total > 15      4grp_total > 38      6grp_cgc > 23      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 175.5
(3)
P 		87.2
(-2.7)
P 		83.3
(-3)
P 		140.2
(0.7)
P 		92
B 136.1
(3)
P 		94.3
(-3)
P 		94.6
(-3)
P 		131.9
(2.4)
P 		42
C 170.4
(3)
P 		141.2
(0.7)
P 		94.4
(-3)
P 		100.9
(-2.5)
P 		76
avr 160.7
(3) 		107.6
(-1.6) 		90.8
(-3) 		124.3
(-0.1) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 115.4
(3)
P 		100.9
(0.8)
P 		75.8
(-3)
P 		79.5
(-2.4)
P 		79
(-2.5)
A 		85.5
(-1.5)
P 		40
B 162.5
(3)
P 		108.3
(0.3)
P 		86.7
(-0.8)
P 		53
(-2.4)
A 		41.5
(-3)
A 		59.5
(-2.1)
P 		121
C 127.2
(2.4)
P 		134
(2.9)
P 		64
(-1.9)
P 		135.8
(3)
P 		47.2
(-3)
P 		69.7
(-1.5)
P 		89
avr 135
(3) 		114.4
(1.4) 		75.5
(-1.5) 		89.4
(-0.5) 		55.9
(-3) 		71.6
(-1.8) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 63.9
(3)
P 		18.9
(-3)
P 		21.8
(-2.6)
P 		20.5
(-2.8)
P 		45
B 43.4
(3)
P 		23.3
(-2.6)
P 		21.9
(-3)
P 		28.1
(-1.3)
P 		22
C 56.4
(3)
P 		40.6
(0.6)
P 		32.9
(-0.6)
P 		17
(-3)
P 		39
avr 54.6
(3) 		27.6
(-2) 		25.5
(-2.3) 		21.9
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 21.3
(-1.5)
P 		35.9
(3)
P 		16.5
(-3)
P 		20.2
(-1.9)
P 		23.8
(-0.7)
A 		20.8
(-1.7)
P 		19
B 56.5
(3)
P 		36
(0.1)
P 		21
(-2)
P 		14
(-3)
A 		16.8
(-2.6)
A 		22.2
(-1.8)
P 		43
C 47.2
(3)
P 		40.9
(1.8)
P 		16.3
(-2.8)
P 		27.3
(-0.7)
P 		15.3
(-3)
P 		23.3
(-1.5)
P 		32
avr 41.7
(3) 		37.6
(2) 		17.9
(-3) 		20.5
(-2.3) 		18.6
(-2.8) 		22.1
(-1.9) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176613_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17571
Sequence Type Exemplar sequence
Representative Public ID W09B12.1 NCBI
Target Description W09B12.1 /REP_DB=WormBase Gene ID /WP=CE07569 /GEN=ace-1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078259(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078259 NCBI Caenorhabditis elegans abnormal ACEtylcholinesterase family member (ace-1) (ace-1) mRNA, complete cds. 11/11 A 
SNAP00000041956 ENSEMBL cdna:SNAP chromosome:CEL140:X:16370305:16373186:1 11/11 None
GENEFINDER00000041970 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16370428:16373186:1 9/11 None
W09B12.1.1 ENSEMBL cdna:known chromosome:CEL140:X:16367145:16373951:1 gene:W09B12.1 11/11 None
W09B12.1.2 ENSEMBL cdna:known chromosome:CEL140:X:16367207:16373186:1 gene:W09B12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16367294-16373186(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ace-1
Ensembl W09B12.1
Entrez Gene 181706 Entrez gene
SwissProt P38433 EMBL-EBI
WormBase CE07569 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21696.1.S1_AT similar to Acetylcholinesterase precursor (AChE) cfa
CANINE_2:CFAAFFX.21697.1.S1_S_AT similar to Acetylcholinesterase precursor (AChE) cfa
HUGENEFL:S71129_AT acetylcholinesterase (YT blood group) hs
HUGENEFL:M55040_AT acetylcholinesterase (YT blood group) hs
HG-U133A:205378_S_AT acetylcholinesterase (YT blood group) hs
HG-U133_PLUS_2:205378_S_AT acetylcholinesterase (YT blood group) hs
HG-U133A_2:205378_S_AT acetylcholinesterase (YT blood group) hs
U133_X3P:G7710111_3P_A_AT acetylcholinesterase (YT blood group) hs
U133_X3P:HS.154495.0.S1_3P_A_AT acetylcholinesterase (YT blood group) hs
HG-U133A_2:205377_S_AT acetylcholinesterase (YT blood group) hs
HG-FOCUS:205377_S_AT acetylcholinesterase (YT blood group) hs
HG-U133_PLUS_2:205377_S_AT acetylcholinesterase (YT blood group) hs
HG-U133A:205377_S_AT acetylcholinesterase (YT blood group) hs
HG-U95AV2:35387_R_AT acetylcholinesterase (YT blood group) hs
HG-U95AV2:40249_AT acetylcholinesterase (YT blood group) hs
HG-U133_PLUS_2:210332_AT acetylcholinesterase (YT blood group) hs
HG-U133A:210332_AT acetylcholinesterase (YT blood group) hs
HG-U133A_2:210332_AT acetylcholinesterase (YT blood group) hs
HG-U95AV2:35386_AT Acetylcholinesterase (YT blood group) hs
MOUSE430A_2:1422635_AT acetylcholinesterase mm
MOE430A:1422635_AT acetylcholinesterase mm
MOUSE430_2:1422635_AT acetylcholinesterase mm
MU11KSUBB:X56518_S_AT acetylcholinesterase mm
MG-U74AV2:104650_AT acetylcholinesterase mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6581 acetylcholine catabolism inferred from direct assay QuickGO AmiGO
1507 acetylcholine catabolism in synaptic cleft inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5623 cell inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4104 cholinesterase activity inferred from electronic annotation QuickGO AmiGO
3990 acetylcholinesterase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00593 Abnormal acetylcholinesterase protein 1 [Caenorhabditis elegans] emb|CAA53080.1| acetylcholinesterase [Caenorhabditis elegans] ref|NP_510660.1| abnormal ACEtylcholinesterase family member (ace-1) [Caenorhabditis elegans] sp|P38433|ACE1_CAEEL Acetylcholinesterase 1 precursor (AChE 1) 0.0
blast Q27459 Acetylcholinesterase 1 precursor (AChE 1) emb|CAE69976.1| Hypothetical protein CBG16374 [Caenorhabditis briggsae] gb|AAB41269.1| acetylcholinesterase [Caenorhabditis briggsae] 0.0
blast AAD02835 acetylcholinesterase precursor [Meloidogyne incognita] 0.0
blast AAB00593 Abnormal acetylcholinesterase protein 1 [Caenorhabditis elegans] emb|CAA53080.1| acetylcholinesterase [Caenorhabditis elegans] ref|NP_510660.1| abnormal ACEtylcholinesterase family member (ace-1) [Caenorhabditis elegans] sp|P38433|ACE1_CAEEL Acetylcholinesterase 1 precursor (AChE 1) 1.0E-140
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 3.3E-54
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 4.6E-41
Sequence
Target Sequence 
>C. ELEGANS:176613_AT
gctgggagactatcattttacttgtagcgttaatgaaatggctctggctcatacgaaaca
cggaggagacacatactattactattttacacacagagcttctcaacaaacatggccaga
atggatgggtgttttacatggttacgaaatcaatttcatttttggagaaccattaaatca
aaaaagattcaactacactgatgaagaacgagagttgagcaatcggtttatgagatattg
ggcgaattttgcaaaaactggagatccgaacaaaaacgaggatggatcatttactcaaga
cgtgtggccaaagtataattctgtttcaatggaatacatgaacatgaccgttgaatcgtc
atatcccagcatgaaacgaatagggcatggtccgaggagaaaggagtgtgcattttggaa
agcgtatttgccgaatttgatggctgcagttgccgacgtcggagacccttatttggtttg
gaagcaacaaatggataaatggcagaacgagtacattacagattggcaatatcatttcga
gcagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGGAGACTATCATTTTACTTGT 63 295 1278 Antisense
GAAATGGCTCTGGCTCATACGAAAC 305 361 1312 Antisense
GGAGGAGACACATACTATTACTATT 96 515 1339 Antisense
ACTATTTTACACACAGAGCTTCTCA 282 101 1358 Antisense
AGAGCTTCTCAACAAACATGGCCAG 58 71 1372 Antisense
GGATGGGTGTTTTACATGGTTACGA 654 507 1400 Antisense
GATCATTTACTCAAGACGTGTGGCC 452 419 1562 Antisense
GACCGTTGAATCGTCATATCCCAGC 198 383 1623 Antisense
GAAACGAATAGGGCATGGTCCGAGG 425 357 1650 Antisense
GAGACCCTTATTTGGTTTGGAAGCA 593 391 1739 Antisense
GATTGGCAATATCATTTCGAGCAGT 663 435 1798 Antisense
Sequence Source Affymetrix Proprietary Database