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[176611_at] 7TM chemoreceptor, srg family

Description T12A2.11 /REP_DB=WormBase Gene ID /WP=CE25993 /GEN=srg-5 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 19      6grp_cgc > 99      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 44.4
(-0.7)
P 		70.7
(2.1)
P 		23
(-3)
A 		79.2
(3)
A 		56
B 75.1
(0.5)
P 		53.3
(-2.5)
A 		49.6
(-3)
A 		93.2
(3)
P 		44
C 39.5
(-3)
A 		66.8
(0.5)
P 		57.3
(-0.7)
A 		86.6
(3)
A 		47
avr 53
(-1.6) 		63.6
(-0.2) 		43.3
(-3) 		86.3
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 99.8
(2.8)
P 		42.4
(-3)
A 		51.4
(-2.1)
P 		57.7
(-1.5)
M 		102
(3)
P 		55.4
(-1.7)
A 		60
B 67
(3)
P 		46.3
(-1.1)
M 		40.8
(-2.2)
A 		36.7
(-3)
A 		64.6
(2.5)
P 		43
(-1.8)
A 		30
C 32.2
(-2.8)
A 		48.1
(0.7)
A 		31.1
(-3)
A 		58.4
(3)
A 		48.7
(0.9)
P 		37.8
(-1.5)
A 		27
avr 66.3
(1.9) 		45.6
(-2.1) 		41.1
(-3) 		50.9
(-1.1) 		71.8
(3) 		45.4
(-2.2) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 16.2
(3)
P 		15.4
(2.5)
P 		6
(-3)
A 		11.6
(0.3)
A 		10
B 23.9
(3)
P 		13.2
(-2.2)
A 		11.5
(-3)
A 		19.9
(1.1)
P 		12
C 13.1
(-3)
A 		19.2
(2.3)
P 		20
(3)
A 		14.6
(-1.7)
A 		7
avr 17.7
(3) 		15.9
(0.9) 		12.5
(-3) 		15.4
(0.3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 18.4
(-0.8)
P 		15.1
(-1.8)
A 		11.2
(-3)
P 		14.6
(-2)
M 		30.8
(3)
P 		13.5
(-2.3)
A 		20
B 23.3
(1.9)
P 		15.4
(-0.9)
M 		9.9
(-2.9)
A 		9.7
(-3)
A 		26.2
(3)
P 		16
(-0.7)
A 		17
C 11.9
(0.1)
A 		14.7
(2.2)
A 		7.9
(-3)
A 		11.7
(-0.1)
A 		15.7
(3)
P 		12.6
(0.6)
A 		8
avr 17.9
(0.4) 		15.1
(-0.8) 		9.7
(-3) 		12
(-2) 		24.2
(3) 		14
(-1.2) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176611_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6828
Sequence Type Exemplar sequence
Representative Public ID T12A2.11 NCBI
Target Description T12A2.11 /REP_DB=WormBase Gene ID /WP=CE25993 /GEN=srg-5 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065968(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065968 NCBI Caenorhabditis elegans Serpentine Receptor, class G (gamma) family member (srg-5) (srg-5) mRNA, complete cds. 11/11 None
GENEFINDER00000044244 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6258529:6263801:-1 10/11 None
T12A2.11 ENSEMBL cdna:known chromosome:CEL140:III:6262448:6263699:-1 gene:T12A2.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000044234 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6262446-6263800(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srg family
Gene Symbol srg-5
Ensembl T12A2.11
Entrez Gene 191831 Entrez gene
SwissProt P54127 EMBL-EBI
WormBase CE33180 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84567 Serpentine receptor, class g (gamma) protein 5 [Caenorhabditis elegans] ref|NP_498369.2| Serpentine Receptor, class G (gamma) family member (srg-5) [Caenorhabditis elegans] sp|P54127|SRG5_CAEEL Serpentine receptor class gamma-5 (Protein srg-5) 1.0E-160
blast 2123261AE chemosensory receptor 1.0E-150
blast 2123261AE chemosensory receptor 1.0E-138
blast AAK84567 Serpentine receptor, class g (gamma) protein 5 [Caenorhabditis elegans] ref|NP_498369.2| Serpentine Receptor, class G (gamma) family member (srg-5) [Caenorhabditis elegans] sp|P54127|SRG5_CAEEL Serpentine receptor class gamma-5 (Protein srg-5) 1.0E-126
blast AAC47052 Serpentine receptor, class g (gamma) protein 2 [Caenorhabditis elegans] ref|NP_498367.2| Serpentine Receptor, class G (gamma) family member (srg-2) [Caenorhabditis elegans] sp|P46571|SRG2_CAEEL Serpentine receptor class gamma-2 (Protein srg-2) 1.0E-120
blast CAE70734 Hypothetical protein CBG17478 [Caenorhabditis briggsae] 1.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.3E-7
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.1E-86
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.7E-114
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 3.3E-34
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 5.6E-77
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498369.2 5 22-44,143-165,192-214,234-256,266-288
T12A2.11 5 22-44,143-165,192-214,234-256,266-288
GENEFINDER00000044244 13 29-48,61-83,98-117,149-171,198-220,240-262,272-294,381-398,471-488,493-515,519-541,554-576,586-608
Sequence
Target Sequence 
>C. ELEGANS:176611_AT
gtgctggcaccattgagatattctcagatctggcgtcgtttcatccctgtcacaatcgct
tttatcacattgtcaccatttctagtaatttggaatgttatcatatccgaaacgtttcct
gtttcaatatttggcggtttcactctggcctacacaaaacgtgttcgctgggcgagtcta
tcaatgttccaaatgattttcatggcaatttctcttacaattactgttttcacaacttct
atcacattattcaaaatgaggagattggaaaatcgattgaaaagttctgagcgaactttg
tgttttgcatcgttttatatgtcggccgcttttttcagtgccgctctttttcaaagctac
tttgcgttcttcagcataactgctgcatacacagatcttgtgtattttcttcaaggcttt
gcatttgatgttctcaacgttggctcaccaattgtgatggttcttatcagtggacaattg
agatatcatgtgattccagtcaaaagcatggctccaaagcattcgacagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCTGGCACCATTGAGATATTCTC 548 479 505 Antisense
TATTCTCAGATCTGGCGTCGTTTCA 105 663 523 Antisense
CCCTGTCACAATCGCTTTTATCACA 448 259 549 Antisense
TATCACATTGTCACCATTTCTAGTA 645 659 567 Antisense
TATTTGGCGGTTTCACTCTGGCCTA 489 659 632 Antisense
TCGCTGGGCGAGTCTATCAATGTTC 296 597 669 Antisense
GTTTTCACAACTTCTATCACATTAT 77 449 730 Antisense
TTATATGTCGGCCGCTTTTTTCAGT 349 679 819 Antisense
AAAGCTACTTTGCGTTCTTCAGCAT 392 123 857 Antisense
TTGATGTTCTCAACGTTGGCTCACC 146 705 929 Antisense
GCATGGCTCCAAAGCATTCGACAGT 276 311 1010 Antisense
Sequence Source Affymetrix Proprietary Database