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[176598_s_at]

Description C05D2.1 /REP_DB=WormBase Gene ID /WP=CE16827 /CHR=3 /FEA=Sanger Annotation /DEF=locus:daf-4 (ST.LOUIS)
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 23      4grp_highest_8_8 > 62      4grp_high_8_8 > 27      6grp_highest_nonorm > 71      6grp_total > 12      4grp_total > 37      6grp_highest_sub > 02      6grp_cgc > 43      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 579.8
(3)
P 		491.3
(1.1)
P 		295.8
(-3)
P 		490.1
(1.1)
P 		284
B 548.9
(3)
P 		460
(0.5)
P 		335.9
(-3)
P 		467.1
(0.7)
P 		213
C 522.5
(3)
P 		454.9
(1)
P 		316.5
(-3)
P 		431.2
(0.3)
P 		206
avr 550.4
(3) 		468.7
(0.9) 		316.1
(-3) 		462.8
(0.8) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 343.5
(-0.7)
P 		509.8
(3)
P 		309.9
(-1.4)
P 		334.6
(-0.9)
P 		314.4
(-1.3)
P 		239.5
(-3)
P 		270
B 432.4
(2.7)
P 		444
(3)
P 		399.6
(1.7)
P 		322.6
(-0.6)
P 		273.7
(-2.1)
P 		244.3
(-3)
P 		200
C 428.9
(1.4)
P 		504.8
(3)
P 		432
(1.5)
P 		249.5
(-2.2)
P 		268.7
(-1.8)
P 		212.2
(-3)
P 		293
avr 401.6
(1) 		486.2
(3) 		380.5
(0.5) 		302.2
(-1.3) 		285.6
(-1.7) 		232
(-3) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 211.1
(3)
P 		106.7
(-1.5)
P 		77.3
(-2.8)
P 		71.7
(-3)
P 		139
B 175.1
(3)
P 		113.6
(-0.8)
P 		77.8
(-3)
P 		99.5
(-1.7)
P 		97
C 173
(3)
P 		130.8
(0.5)
P 		110.3
(-0.8)
P 		72.7
(-3)
P 		100
avr 186.4
(3) 		117
(-1) 		88.5
(-2.6) 		81.3
(-3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 63.3
(-2.8)
P 		181.3
(3)
P 		67.5
(-2.6)
P 		84.9
(-1.7)
P 		94.9
(-1.2)
P 		58.3
(-3)
P 		123
B 150.5
(3)
P 		147.8
(2.8)
P 		97
(-1.9)
P 		85.5
(-3)
P 		111.2
(-0.6)
P 		91.1
(-2.5)
P 		65
C 159
(3)
P 		154
(2.7)
P 		110
(0.3)
P 		50.2
(-3)
P 		86.9
(-1)
P 		71
(-1.9)
P 		109
avr 124.3
(0.5) 		161
(3) 		91.5
(-1.8) 		73.5
(-3) 		97.7
(-1.3) 		73.5
(-3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176598_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6722
Sequence Type Exemplar sequence
Representative Public ID C05D2.1 NCBI
Target Description C05D2.1 /REP_DB=WormBase Gene ID /WP=CE16827 /CHR=3 /FEA=Sanger Annotation /DEF=locus:daf-4 (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065810(11), NM_001025977(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065810 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-4) (daf-4) mRNA, complete cds. 11/11 A 
NM_001025977 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-4) (daf-4) mRNA, complete cds. 11/11 None
GENEFINDER00000007740 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5625094:5631795:1 11/11 None
SNAP00000007739 ENSEMBL cdna:SNAP chromosome:CEL140:III:5628604:5631795:1 11/11 None
C05D2.1a ENSEMBL cdna:known chromosome:CEL140:III:5624968:5632372:1 gene:C05D2.1 11/11 A 
C05D2.1c ENSEMBL cdna:known chromosome:CEL140:III:5626438:5631795:1 gene:C05D2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175781 3 2 NM_001025977 176598_s_at (11)
NM_065810 175667_at (11), 176598_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5625092-5631794(+) 100.0 100.0
Public Domain and Genome References
Ensembl C05D2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC02726 Abnormal dauer formation protein 4, isoform a [Caenorhabditis elegans] ref|NP_498211.1| abnormal DAuer Formation family member (daf-4) [Caenorhabditis elegans] sp|P50488|DAF4_CAEEL Cell-surface receptor daf-4 precursor (Abnormal dauer formation protein 4) 0.0
blast 1922354A bone morphogenetic protein receptor gb|AAA03544.1| BMP receptor 0.0
blast AAN63460 Abnormal dauer formation protein 4, isoform c [Caenorhabditis elegans] ref|NP_001021148.1| abnormal DAuer Formation family member (daf-4) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-38
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-38
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-38
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498211.1 1 230-252
NP_001021148.1 1 78-100
C05D2.1a 1 230-252
C05D2.1c 1 78-100
GENEFINDER00000007740 1 209-231
Sequence
Target Sequence 
>C. ELEGANS:176598_S_AT
ggatttgatcccacaattggtctcatgagaaattatgtggtgagcaagaaggaacgacca
caatggagagatgagattattaaacatgaatatatgagtctgctaaaaaaggttacggaa
gagatgtgggatcccgaagcgtgtgcacggattacagctggatgtgcgttcgcgagggta
tggaatcatattatgagctcaccggacagttcggaggggtatcacagtggaagtagtatg
aagaatcggggtgttgatgatgttgaacaaagtgagaagccggagggtatcgaagaaatg
caacattatcatgcgtcgtcacccagcaagagacaacatcctagtccgaatccgtttttt
gattcgtgtccaccacctccgccgattcctgtgatccttgaaaatggaggaatcctccaa
ccagacaacgcggagccagagcccgaagagctgccggatctcccgatagtcgagaaaatc
tacgatattgcgacgaacatgctattctcaagagaagagcttgaccttatgaatgctcaa
cggcaagttgaatacgaagctggggcagacactcgagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTTGATCCCACAATTGGTCTCA 108 511 1624 Antisense
GATGTGGGATCCCGAAGCGTGTGCA 691 407 1746 Antisense
AAGCGTGTGCACGGATTACAGCTGG 184 149 1760 Antisense
TTACAGCTGGATGTGCGTTCGCGAG 159 683 1775 Antisense
TGAGCTCACCGGACAGTTCGGAGGG 66 565 1817 Antisense
TGCAACATTATCATGCGTCGTCACC 432 577 1922 Antisense
CATCCTAGTCCGAATCCGTTTTTTG 693 207 1960 Antisense
GCCGATTCCTGTGATCCTTGAAAAT 230 285 2004 Antisense
TCCAACCAGACAACGCGGAGCCAGA 693 585 2039 Antisense
TGCCGGATCTCCCGATAGTCGAGAA 276 583 2075 Antisense
ACGAAGCTGGGGCAGACACTCGAGC 242 91 2177 Antisense
Sequence Source Affymetrix Proprietary Database