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[176597_at]

Description JC8.4 /REP_DB=WormBase Gene ID /WP=CE17987 /GB=CAB05231.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 76      4grp_high_8_8 > 21      6grp_total > 77      4grp_total > 04      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 95.6
(-3)
P 		129.8
(-1.5)
P 		102.6
(-2.7)
P 		232.2
(3)
P 		137
B 135.3
(1.3)
P 		54.6
(-3)
A 		76.1
(-1.8)
P 		166.1
(3)
P 		112
C 107.4
(-1.5)
P 		80.8
(-3)
A 		132.2
(-0.1)
P 		188.1
(3)
P 		107
avr 112.8
(-1.6) 		88.4
(-3) 		103.6
(-2.1) 		195.5
(3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 103.2
(-1.9)
A 		103.2
(-1.9)
P 		106.3
(-1.6)
P 		131.7
(0.5)
P 		89.7
(-3)
P 		161.1
(3)
P 		71
B 112.5
(-0.3)
M 		103.5
(-0.8)
P 		121.2
(0.3)
P 		67.2
(-3)
P 		142.5
(1.6)
P 		166.2
(3)
P 		99
C 92.2
(-3)
P 		111.8
(-1.9)
P 		140.1
(-0.4)
P 		117.8
(-1.6)
P 		106.9
(-2.2)
P 		204.1
(3)
P 		112
avr 102.6
(-3) 		106.2
(-2.7) 		122.5
(-1.4) 		105.6
(-2.8) 		113
(-2.2) 		177.1
(3) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 34.8
(3)
P 		28.2
(-2)
P 		26.8
(-3)
P 		34
(2.4)
P 		8
B 43.1
(3)
P 		13.5
(-3)
A 		17.6
(-2.2)
P 		35.4
(1.4)
P 		30
C 35.6
(0.2)
P 		23.2
(-3)
A 		46.1
(3)
P 		31.7
(-0.8)
P 		23
avr 37.8
(3) 		21.6
(-3) 		30.2
(0.2) 		33.7
(1.5) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 19
(-3)
A 		36.7
(2.3)
P 		23.2
(-1.8)
P 		33.4
(1.3)
P 		27.1
(-0.6)
P 		39.2
(3)
P 		20
B 39.1
(-0.1)
M 		34.4
(-0.7)
P 		29.4
(-1.4)
P 		17.8
(-3)
P 		57.9
(2.4)
P 		62
(3)
P 		44
C 34.2
(-1.6)
P 		34.1
(-1.6)
P 		35.7
(-1.4)
P 		23.7
(-3)
P 		34.6
(-1.5)
P 		68.3
(3)
P 		45
avr 30.8
(-1.9) 		35.1
(-1.1) 		29.4
(-2.2) 		25
(-3) 		39.9
(-0.2) 		56.5
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176597_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10120
Sequence Type Exemplar sequence
Representative Public ID JC8.4 NCBI
Target Description JC8.4 /REP_DB=WormBase Gene ID /WP=CE17987 /GB=CAB05231.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070145(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070145 NCBI Caenorhabditis elegans JC8.4 (JC8.4) mRNA, complete cds. 11/11 None
SNAP00000009001 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13243644:13244174:1 11/11 None
GENEFINDER00000009013 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13243644:13244174:1 11/11 None
JC8.4 ENSEMBL cdna:known chromosome:CEL140:IV:13243644:13244174:1 gene:JC8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13243649-13244180(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl JC8.4
Entrez Gene 178281 Entrez gene
SwissProt Q95QD6 EMBL-EBI
WormBase CE17987 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1629942_AT dm
DROSGENOME1:150059_AT dm
CHICKEN:GGA.12278.1.S1_A_AT deaminase domain containing 1 gga
CHICKEN:GGA.12278.2.S1_AT deaminase domain containing 1 gga
U133_X3P:HS.119976.0.S1_3P_AT deaminase domain containing 1 hs
HG-U133_PLUS_2:227213_AT deaminase domain containing 1 hs
HG-U133B:227213_AT deaminase domain containing 1 hs
HG-U95E:69244_AT deaminase domain containing 1 hs
HG-U95E:88657_AT deaminase domain containing 1 hs
HG-U133_PLUS_2:237511_AT Deaminase domain containing 1 hs
HG-U133B:237511_AT Deaminase domain containing 1 hs
HG-U95B:43651_AT Deaminase domain containing 1 hs
HU35KSUBB:RC_N55374_AT Deaminase domain containing 1 hs
U133_X3P:HS.19918.0.A1_3P_AT Deaminase domain containing 1 hs
MOE430A:1419130_AT deaminase domain containing 1 mm
MOUSE430A_2:1419130_AT deaminase domain containing 1 mm
MOUSE430_2:1419130_AT deaminase domain containing 1 mm
RAE230B:1391943_AT Deaminase domain containing 1 (predicted) rn
RAT230_2:1391943_AT Deaminase domain containing 1 (predicted) rn
YEAST_2:1775821_AT Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05231 Hypothetical protein JC8.4 [Caenorhabditis elegans] ref|NP_502546.1| JC8.4 [Caenorhabditis elegans] 7.0E-98
blast CAE58636 Hypothetical protein CBG01804 [Caenorhabditis briggsae] 1.0E-75
blast XP_419709 PREDICTED: similar to dJ20N2.1.1 (novel protein similar to yeast and bacterial cytosine deaminase, variant 1) [Gallus gallus] 8.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR002125 EMBL-EBI Cytidine/deoxycytidylate deaminase, zinc-binding region 8.2E-34
Sequence
Target Sequence 
>C. ELEGANS:176597_AT
gaaaacgagctgagcgccgaggacactgcatttctggaaaaagccttcgaattggctcaa
gaagctttagatgaagatgaagttccagttggatgtgtatttgtggtcaatggaacagaa
attggaagaggtcggaatcgtgtgaacgagaccggtgatccgacaaggcatgctgagatg
gtagcagtcactgaaatgtggaaagagcacggaacgggctgcgaggatctgctcaaaaaa
tccacgctctacgtttccctggagccctgcataatgtgctcgtcggcaatgtatcagctg
ggaattcgaaaaatggtgtatggagccgaaaatccacgattcggaggtgtacggagtgtt
ggaagtgcggagaagtaccgaatggaggataatgtgcaaattgtggctggaatttggtcg
gaacgatctgtcgccatgctgaaaacattctacgagaagcttaacccatttgcaccgcct
gaaaagcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACGAGCTGAGCGCCGAGGACA 93 355 19 Antisense
GCCGAGGACACTGCATTTCTGGAAA 328 285 34 Antisense
GTGTGAACGAGACCGGTGATCCGAC 360 487 158 Antisense
GGTGATCCGACAAGGCATGCTGAGA 508 499 172 Antisense
GAAAGAGCACGGAACGGGCTGCGAG 348 359 219 Antisense
CTGCTCAAAAAATCCACGCTCTACG 266 235 247 Antisense
AGCCCTGCATAATGTGCTCGTCGGC 100 83 281 Antisense
GCTCGTCGGCAATGTATCAGCTGGG 469 297 296 Antisense
GTGGCTGGAATTTGGTCGGAACGAT 327 491 421 Antisense
AACGATCTGTCGCCATGCTGAAAAC 112 143 440 Antisense
ACCCATTTGCACCGCCTGAAAAGCG 144 89 482 Antisense
Sequence Source Affymetrix Proprietary Database