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[176594_s_at]

Description Y104H12BR.B /REP_DB=WormBase Gene ID /WP=CE23180 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 26      4grp_total > 39      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 151.1
(3)
A 		72.4
(-1.3)
A 		41.4
(-3)
A 		132.7
(2)
A 		110
B 189.3
(3)
A 		40.5
(-3)
A 		53.7
(-2.5)
A 		71.7
(-1.7)
A 		149
C 214.7
(3)
A 		85.1
(-2.4)
A 		71.1
(-3)
A 		103.6
(-1.6)
A 		144
avr 185
(3) 		66
(-2.5) 		55.4
(-3) 		102.7
(-0.8) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 7.3
(-3)
A 		71.1
(3)
A 		19.9
(-1.8)
A 		46
(0.6)
A 		7.9
(-2.9)
A 		30.5
(-0.8)
A 		64
B 60.6
(2.2)
A 		68.9
(3)
A 		55.8
(1.7)
A 		6.4
(-3)
A 		48.6
(1.1)
A 		49.9
(1.2)
A 		63
C 128.9
(3)
A 		38.1
(-1.5)
A 		8.8
(-3)
A 		39.3
(-1.5)
A 		51.5
(-0.9)
A 		33.4
(-1.8)
A 		120
avr 65.6
(3) 		59.4
(2) 		28.2
(-3) 		30.6
(-2.6) 		36
(-1.7) 		37.9
(-1.4) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 55
(3)
A 		15.7
(-2.3)
A 		10.8
(-3)
A 		19.4
(-1.8)
A 		44
B 60.4
(3)
A 		10
(-3)
A 		12.4
(-2.7)
A 		15.3
(-2.4)
A 		50
C 71.1
(3)
A 		24.5
(-2.2)
A 		24.8
(-2.2)
A 		17.5
(-3)
A 		54
avr 62.2
(3) 		16.7
(-2.9) 		16
(-3) 		17.4
(-2.8) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 1.3
(-3)
A 		25.3
(3)
A 		4.3
(-2.3)
A 		11.7
(-0.4)
A 		2.4
(-2.7)
A 		7.4
(-1.5)
A 		24
B 21.1
(2.5)
A 		22.9
(3)
A 		13.5
(0.3)
A 		1.7
(-3)
A 		19.7
(2.1)
A 		18.6
(1.8)
A 		21
C 47.8
(3)
A 		11.6
(-1.8)
A 		2.2
(-3)
A 		7.9
(-2.3)
A 		16.7
(-1.1)
A 		11.2
(-1.8)
A 		46
avr 23.4
(3) 		19.9
(1.7) 		6.7
(-3) 		7.1
(-2.9) 		12.9
(-0.8) 		12.4
(-1) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176594_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19198
Sequence Type Exemplar sequence
Representative Public ID Y104H12BR.B NCBI
Target Description Y104H12BR.B /REP_DB=WormBase Gene ID /WP=CE23180 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075650 NCBI Caenorhabditis elegans Y73B3B.1 (Y73B3B.1) mRNA, complete cds. 11/11 None
SNAP00000031494 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1291441:1295725:1 11/11 None
GENEFINDER00000035185 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:209108:211343:-1 11/11 None
SNAP00000035181 ENSEMBL cdna:SNAP chromosome:CEL140:X:209109:215586:-1 11/11 None
Y73B3B.1 ENSEMBL cdna:known chromosome:CEL140:X:209108:211241:-1 gene:Y73B3B.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067660 7/11 Cross Hyb Matching Probes None
GENEFINDER00000031496 8/11 Cross Hyb Matching Probes None
Y104H12BR.1 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1292051-1295732(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y73B3B.1
Entrez Gene 190640 Entrez gene
SwissProt Q95XD8 EMBL-EBI
WormBase CE26598 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39373 Hypothetical protein Y73B3B.1 [Caenorhabditis elegans] ref|NP_508051.1| Y73B3B.1 [Caenorhabditis elegans] 0.0
blast AAK39374 Hypothetical protein Y73B3B.2 [Caenorhabditis elegans] ref|NP_508049.1| Y73B3B.2 [Caenorhabditis elegans] 1.0E-136
blast CAE68269 Hypothetical protein CBG13946 [Caenorhabditis briggsae] 1.0E-131
blast CAB55010 Hypothetical protein Y57A10A.5 [Caenorhabditis elegans] ref|NP_496585.1| Y57A10A.5 [Caenorhabditis elegans] 1.0E-112
blast CAB55010 Hypothetical protein Y57A10A.5 [Caenorhabditis elegans] ref|NP_496585.1| Y57A10A.5 [Caenorhabditis elegans] 1.0E-110
blast CAB55010 Hypothetical protein Y57A10A.5 [Caenorhabditis elegans] ref|NP_496585.1| Y57A10A.5 [Caenorhabditis elegans] 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 3.2E-24
Pfam IPR006570 EMBL-EBI SPK 9.5E-34
Pfam IPR006570 EMBL-EBI SPK 2.3E-66
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.8E-24
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.1E-24
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 3.2E-24
Pfam IPR006570 EMBL-EBI SPK 6.6E-41
Pfam IPR006570 EMBL-EBI SPK 2.3E-66
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 3.2E-24
Pfam IPR006570 EMBL-EBI SPK 6.1E-40
Pfam IPR006570 EMBL-EBI SPK 2.3E-66
Pfam IPR001214 EMBL-EBI Nuclear protein SET 3.9E-11
Sequence
Target Sequence 
>C. ELEGANS:176594_S_AT
attgacattagctctcgtttggcagctaatgcgtgcctataccctctcagttcttgctca
atgtactcaatctggtgattccctgccagctgataaggatattgttgcatgggttaacga
aaagctcaaaaattctggaaaatccacatcaattcgatcgttccaagacccggcaatttc
cgatggaaaagttgtattggatctgattgatgcaattaaaccaaacgttattgatcatag
tttggtgaaaagtggcaaaagtaatgaggataaaatgtcaaatgcaaaatatgcaatcac
ttgtggacgaaagattggagcaaagatttacgcgttgccagaggatattgttgaggtgaa
gccaaaaatggtgttgacagtgtttgcatgtctaatggctcgtgactatcttcccgatat
gaagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGACATTAGCTCTCGTTTGGCAG 415 5 399 Antisense
GCTCTCGTTTGGCAGCTAATGCGTG 215 297 409 Antisense
GGCAGCTAATGCGTGCCTATACCCT 139 535 419 Antisense
GTTCCAAGACCCGGCAATTTCCGAT 245 437 558 Antisense
GCAAAATATGCAATCACTTGTGGAC 275 319 682 Antisense
GGAGCAAAGATTTACGCGTTGCCAG 685 519 715 Antisense
TACGCGTTGCCAGAGGATATTGTTG 671 647 727 Antisense
AATGGTGTTGACAGTGTTTGCATGT 379 163 765 Antisense
GTGTTTGCATGTCTAATGGCTCGTG 122 485 778 Antisense
TCTAATGGCTCGTGACTATCTTCCC 317 613 789 Antisense
GTGACTATCTTCCCGATATGAAGCA 378 483 800 Antisense
Sequence Source Affymetrix Proprietary Database