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[176588_at]

Description Y104H12BL.A /REP_DB=WormBase Gene ID /WP=CE26481 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 09      4grp_total > 32      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 20.2
(0.5)
A 		6.7
(-3)
A 		14.4
(-1)
A 		29.9
(3)
A 		23
B 7.4
(-2.1)
A 		15.4
(3)
A 		6.6
(-2.6)
A 		6
(-3)
A 		9
C 9.8
(-2.5)
A 		15
(-0.6)
A 		25.2
(3)
A 		8.4
(-3)
A 		17
avr 12.5
(-2.8) 		12.4
(-3) 		15.4
(3) 		14.8
(1.8) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 8.4
(0.9)
A 		8.9
(1.3)
A 		3.3
(-3)
A 		6.6
(-0.5)
A 		11.1
(3)
A 		3.5
(-2.8)
A 		8
B 5.2
(0.6)
A 		2.3
(-3)
A 		4.2
(-0.7)
A 		5.9
(1.4)
A 		4.6
(-0.2)
A 		7.2
(3)
A 		5
C 16.5
(3)
A 		2.5
(-3)
A 		15.7
(2.7)
A 		7.9
(-0.7)
A 		3.8
(-2.4)
A 		6.2
(-1.4)
A 		14
avr 10
(3) 		4.6
(-3) 		7.7
(0.4) 		6.8
(-0.6) 		6.5
(-0.9) 		5.6
(-1.9) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 7.3
(3)
A 		1.4
(-3)
A 		3.8
(-0.6)
A 		4.4
(0.1)
A 		6
B 2.4
(-0.4)
A 		3.8
(3)
A 		1.5
(-2.5)
A 		1.3
(-3)
A 		3
C 3.3
(-1.5)
A 		4.3
(-0.6)
A 		8.8
(3)
A 		1.4
(-3)
A 		7
avr 4.3
(2) 		3.2
(-0.9) 		4.7
(3) 		2.4
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 1.5
(-1.2)
A 		3.1
(2.3)
A 		0.7
(-3)
A 		1.7
(-0.8)
A 		3.4
(3)
A 		0.9
(-2.6)
A 		3
B 1.8
(0.2)
A 		0.8
(-3)
A 		1
(-2.4)
A 		1.6
(-0.5)
A 		1.9
(0.5)
A 		2.7
(3)
A 		2
C 6.1
(3)
A 		0.8
(-3)
A 		4
(0.6)
A 		1.6
(-2.1)
A 		1.2
(-2.5)
A 		2.1
(-1.5)
A 		5
avr 3.1
(3) 		1.6
(-3) 		1.9
(-1.8) 		1.6
(-3) 		2.2
(-0.6) 		1.9
(-1.8) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176588_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19368
Sequence Type Exemplar sequence
Representative Public ID Y104H12BL.A NCBI
Target Description Y104H12BL.A /REP_DB=WormBase Gene ID /WP=CE26481 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067659(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067659 NCBI Caenorhabditis elegans Y104H12BL.1 (Y104H12BL.1) mRNA, complete cds. 11/11 None
SNAP00000040424 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1289629:1290017:1 9/11 None
GENEFINDER00000040425 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1288769:1290017:1 10/11 None
Y104H12BL.1 ENSEMBL cdna:known chromosome:CEL140:IV:1289297:1291045:1 gene:Y104H12BL.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1289302-1290055(+) 89.61 100.0
Public Domain and Genome References
Gene Title
Ensembl Y104H12BL.1
Entrez Gene 190866 Entrez gene
SwissProt Q965Y6 EMBL-EBI
WormBase CE26481 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85497 Hypothetical protein Y104H12BL.1 [Caenorhabditis elegans] ref|NP_500060.1| Y104H12BL.1 [Caenorhabditis elegans] 1.0E-103
blast AAK85497 Hypothetical protein Y104H12BL.1 [Caenorhabditis elegans] ref|NP_500060.1| Y104H12BL.1 [Caenorhabditis elegans] 1.0E-83
blast AAK85497 Hypothetical protein Y104H12BL.1 [Caenorhabditis elegans] ref|NP_500060.1| Y104H12BL.1 [Caenorhabditis elegans] 2.0E-70
blast CAE68269 Hypothetical protein CBG13946 [Caenorhabditis briggsae] 3.0E-63
blast CAE68269 Hypothetical protein CBG13946 [Caenorhabditis briggsae] 6.0E-63
blast CAE68269 Hypothetical protein CBG13946 [Caenorhabditis briggsae] 3.0E-62
blast XP_392230 PREDICTED: similar to ENSANGP00000011155 [Apis mellifera] 1.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 6.7E-17
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 6.7E-17
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 6.7E-17
Sequence
Target Sequence 
>C. ELEGANS:176588_AT
gttgatttttttgccctaattgctcaagttttcgattttttccaattttttcgagttttt
tgctctaaaaactctgaaaatccacaaatttccagactgctccgcgacggtgaaactctt
gaagatctccggcgtctgtccccagaagaaattctcatgagatgggttaattatcatttg
gaacgtgctggaactcaacgtcgcctacacaatttcacatcagatattgtcgattcagag
atttatactcatttgctccatcaaattgctccaaatggctccggtgtcacgttatctcca
ctcggagtccatggaaatgtcccaagagccggagcaatgttagatgaagccgagaagctt
gattgtcgggaatttgtgacagcaactgatgtggcagccggaaattacaaattaaacttg
gcttttgttgccaatttgttcaataaacatccaaatttgccggatccgggagcggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGATTTTTTTGCCCTAATTGCTC 508 435 94 Antisense
AATCCACAAATTTCCAGACTGCTCC 509 167 172 Antisense
CTGCTCCGCGACGGTGAAACTCTTG 517 237 190 Antisense
GAAACTCTTGAAGATCTCCGGCGTC 265 357 205 Antisense
GAACGTGCTGGAACTCAACGTCGCC 415 347 274 Antisense
GTCGCCTACACAATTTCACATCAGA 317 475 293 Antisense
GATTTATACTCATTTGCTCCATCAA 412 429 333 Antisense
TCACGTTATCTCCACTCGGAGTCCA 22 625 380 Antisense
TGGAAATGTCCCAAGAGCCGGAGCA 531 547 405 Antisense
GGCTTTTGTTGCCAATTTGTTCAAT 266 537 513 Antisense
AAATTTGCCGGATCCGGGAGCGGAT 494 113 546 Antisense
Sequence Source Affymetrix Proprietary Database