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[176585_at]

Description Y57E12AM.1 /REP_DB=WormBase Gene ID /WP=CE26193 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 83      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 46      6grp_total > 09      4grp_total > 58      6grp_high1_sub > 04      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1168
1 2 3 4 range
A 2797.1
(-1.9)
P 		3058.2
(1)
P 		3234
(3)
P 		2703
(-3)
P 		531
B 2997.5
(-0.5)
P 		3275.3
(1.2)
P 		3574.1
(3)
P 		2589
(-3)
P 		985
C 2692.2
(-1.5)
P 		3530
(3)
P 		3193.3
(1.2)
P 		2405.9
(-3)
P 		1124
avr 2828.9
(-0.9) 		3287.8
(2.6) 		3333.8
(3) 		2566
(-3) 		768

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1189
1 2 3 4 5 6 range
A 3297.7
(3)
P 		2472.5
(-3)
P 		3229.4
(2.5)
P 		2960
(0.5)
P 		3052.2
(1.2)
P 		2649.4
(-1.7)
P 		825
B 3296.7
(3)
P 		2195.1
(-3)
P 		3030.1
(1.5)
P 		3133.6
(2.1)
P 		3114.2
(2)
P 		2831.4
(0.5)
P 		1102
C 2872.3
(1.4)
P 		2108.8
(-3)
P 		2804.9
(1.1)
P 		2986
(2.1)
P 		2664.4
(0.2)
P 		3138.7
(3)
P 		1030
avr 3155.6
(3) 		2258.8
(-3) 		3021.5
(2.1) 		3026.5
(2.1) 		2943.6
(1.6) 		2873.2
(1.1) 		897
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 717
1 2 3 4 range
A 1018.4
(3)
P 		663.8
(-0.4)
P 		845.4
(1.3)
P 		395.7
(-3)
P 		623
B 955.9
(3)
P 		808.9
(0.8)
P 		828.2
(1.1)
P 		551.7
(-3)
P 		404
C 891.2
(1.1)
P 		1014.9
(2.2)
P 		1113
(3)
P 		405.7
(-3)
P 		707
avr 955.2
(3) 		829.2
(1.5) 		928.9
(2.7) 		451
(-3) 		504

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 665
1 2 3 4 5 6 range
A 607.9
(-3)
P 		879.3
(2.2)
P 		703.3
(-1.2)
P 		750.7
(-0.3)
P 		921
(3)
P 		645
(-2.3)
P 		313
B 1147.1
(1.7)
P 		730.7
(-3)
P 		735.8
(-2.9)
P 		830
(-1.9)
P 		1265.3
(3)
P 		1055.8
(0.6)
P 		535
C 1064.6
(3)
P 		643.4
(-2.4)
P 		713.9
(-1.5)
P 		600.5
(-3)
P 		862.2
(0.4)
P 		1050.7
(2.8)
P 		464
avr 939.9
(1.5) 		751.1
(-2.3) 		717.7
(-3) 		727.1
(-2.8) 		1016.2
(3) 		917.2
(1) 		299
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176585_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11885
Sequence Type Exemplar sequence
Representative Public ID Y57E12AM.1 NCBI
Target Description Y57E12AM.1 /REP_DB=WormBase Gene ID /WP=CE26193 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072393(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072393 NCBI Caenorhabditis elegans Y57E12AM.1 (Y57E12AM.1) mRNA, complete cds. 11/11 None
SNAP00000015458 ENSEMBL cdna:SNAP chromosome:CEL140:V:7065788:7066130:1 10/11 None
GENEFINDER00000015460 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7065788:7066130:1 10/11 None
Y57E12AM.1 ENSEMBL cdna:known chromosome:CEL140:V:7065765:7066255:1 gene:Y57E12AM.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7065773-7066131(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y57E12AM.1
Entrez Gene 179094 Entrez gene
WormBase CE26193 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85505 Hypothetical protein Y57E12AM.1 [Caenorhabditis elegans] ref|NP_504794.1| Y57E12AM.1 [Caenorhabditis elegans] sp|Q965T1|U197_CAEEL Hypothetical UPF0197 protein Y57E12AM.1 in chromosom V 9.0E-26
blast AAK85505 Hypothetical protein Y57E12AM.1 [Caenorhabditis elegans] ref|NP_504794.1| Y57E12AM.1 [Caenorhabditis elegans] sp|Q965T1|U197_CAEEL Hypothetical UPF0197 protein Y57E12AM.1 in chromosom V 5.0E-23
blast CAE71997 Hypothetical protein CBG19073 [Caenorhabditis briggsae] 1.0E-20
blast CAE71997 Hypothetical protein CBG19073 [Caenorhabditis briggsae] 7.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR007915 EMBL-EBI Protein of unknown function UPF0197 5.9E-58
Pfam IPR007915 EMBL-EBI Protein of unknown function UPF0197 6.5E-55
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504794.1 2 13-35,55-77
Y57E12AM.1 2 13-35,55-77
SNAP00000015458 2 12-34,49-71
GENEFINDER00000015460 2 12-34,49-71
Sequence
Target Sequence 
>C. ELEGANS:176585_AT
aagatgaacagatacaccgctccagtaaacttcgcgagccttccactgctcaccacgttt
ctctgtggagttggtcttcttctgctcgccacgttcaccatgatccaggttacatcgacg
aaatacaaccgtaaccttctgaaggaactcttcatcgccgccacatcatcagtcttcctt
ggct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGATGAACAGATACACCGCTCCAG 133 155 25 Antisense
TACACCGCTCCAGTAAACTTCGCGA 298 641 37 Antisense
GCTCCAGTAAACTTCGCGAGCCTTC 44 299 43 Antisense
TGCTCACCACGTTTCTCTGTGGAGT 345 581 71 Antisense
CCACGTTCACCATGATCCAGGTTAC 136 273 113 Antisense
CACCATGATCCAGGTTACATCGACG 205 201 120 Antisense
GATCCAGGTTACATCGACGAAATAC 275 413 126 Antisense
GACGAAATACAACCGTAACCTTCTG 179 377 141 Antisense
GAAATACAACCGTAACCTTCTGAAG 583 353 144 Antisense
TCTGAAGGAACTCTTCATCGCCGCC 398 607 162 Antisense
GCCACATCATCAGTCTTCCTTGGCT 97 279 184 Antisense
Sequence Source Affymetrix Proprietary Database