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[176577_s_at]

Description ZK370.2 /REP_DB=WormBase Gene ID /WP=CE25686 /GEN=sma-2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 31      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 234
1 2 3 4 range
A 197.4
(3)
P 		97.9
(-3)
M 		101.6
(-2.8)
A 		190.1
(2.6)
P 		100
B 196.7
(3)
P 		140.8
(0.4)
A 		69.1
(-3)
P 		183.6
(2.4)
P 		128
C 303
(3)
P 		80.8
(-3)
A 		110.4
(-2.2)
P 		167.5
(-0.7)
P 		222
avr 232.4
(3) 		106.5
(-2.4) 		93.7
(-3) 		180.4
(0.8) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 103
(0.2)
P 		88
(-0.8)
P 		60.1
(-2.7)
P 		146
(3)
M 		54.8
(-3)
A 		83.4
(-1.1)
A 		91
B 100.7
(3)
P 		91
(1.8)
P 		80.4
(0.6)
P 		95.2
(2.3)
P 		77.5
(0.2)
A 		50.1
(-3)
A 		51
C 141.4
(3)
P 		85.2
(-1)
P 		57.2
(-3)
A 		109.8
(0.7)
P 		109.6
(0.7)
A 		62
(-2.7)
P 		84
avr 115
(2.8) 		88.1
(-0.3) 		65.9
(-2.9) 		117
(3) 		80.6
(-1.2) 		65.2
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 71.9
(3)
P 		21.2
(-3)
M 		26.6
(-2.4)
A 		27.8
(-2.2)
P 		51
B 62.7
(3)
P 		34.8
(-0.6)
A 		16
(-3)
P 		39.1
(-0)
P 		47
C 100.3
(3)
P 		23.2
(-3)
A 		38.5
(-1.8)
P 		28.2
(-2.6)
P 		77
avr 78.3
(3) 		26.4
(-3) 		27
(-2.9) 		31.7
(-2.4) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 19
(-1.5)
P 		31.3
(1.6)
P 		13.1
(-3)
P 		37
(3)
M 		16.5
(-2.1)
A 		20.3
(-1.2)
A 		24
B 35
(3)
P 		30.3
(1.3)
P 		19.5
(-2.7)
P 		25.2
(-0.6)
P 		31.5
(1.7)
A 		18.7
(-3)
A 		16
C 52.4
(3)
P 		26
(-1.2)
P 		14.6
(-3)
A 		22.1
(-1.8)
P 		35.5
(0.3)
A 		20.8
(-2)
P 		38
avr 35.5
(3) 		29.2
(1.1) 		15.7
(-3) 		28.1
(0.8) 		27.8
(0.7) 		19.9
(-1.7) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176577_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7490
Sequence Type Exemplar sequence
Representative Public ID ZK370.2 NCBI
Target Description ZK370.2 /REP_DB=WormBase Gene ID /WP=CE25686 /GEN=sma-2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066530(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066530 NCBI Caenorhabditis elegans SMAll family member (sma-2) (sma-2) mRNA, complete cds. 11/11 A 
ZK370.2 ENSEMBL cdna:known chromosome:CEL140:III:8749674:8756971:1 gene:ZK370.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000027078 2/11 Cross Hyb Matching Probes None
SNAP00000027073 2/11 Cross Hyb Matching Probes None
GENEFINDER00000004302 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8750547-8755625(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sma-2
Ensembl ZK370.2
Entrez Gene 176229 Entrez gene
SwissProt Q02330 EMBL-EBI
WormBase CE33015 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.10232.1.S1_S_AT similar to Mothers against decapentaplegic homolog 9 (SMAD 9) (Mothers against DPP homolog 9) (Smad9) (Madh6) cfa
CHICKEN:GGA.293.1.S1_AT SMAD, mothers against DPP homolog 9 (Drosophila) gga
HG-U133_PLUS_2:206320_S_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
HG-U133A:206320_S_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
HG-FOCUS:206320_S_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
HG-U133A_2:206320_S_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
U133_X3P:G5174518_3P_A_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
HG-U95AV2:32986_S_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
HG-U95E:84925_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
HG-U95C:50572_AT SMAD, mothers against DPP homolog 9 (Drosophila) hs
MOUSE430_2:1450265_AT MAD homolog 9 (Drosophila) mm
MOUSE430A_2:1450265_AT MAD homolog 9 (Drosophila) mm
MOE430A:1450265_AT MAD homolog 9 (Drosophila) mm
MG-U74BV2:110524_AT MAD homolog 9 (Drosophila) mm
RN-U34:AF012347_AT MAD homolog 9 (Drosophila) rn
RG-U34A:AF012347_AT MAD homolog 9 (Drosophila) rn
RAE230A:1370716_AT MAD homolog 9 (Drosophila) rn
RAT230_2:1370716_AT MAD homolog 9 (Drosophila) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA28204 Small protein 2 [Caenorhabditis elegans] ref|NP_498931.2| SMAll family member (sma-2) [Caenorhabditis elegans] sp|Q02330|SMA2_CAEEL Dwarfin sma-2 (MAD protein homolog 1) gb|AAC46583.1| MAD homolog 1 gb|AAA97606.1| dwarfin family member; Allele: wildtype (N2); Method: conceptual translation supplied by author 0.0
blast CAE62756 Hypothetical protein CBG06922 [Caenorhabditis briggsae] 0.0
blast XP_880723 PREDICTED: similar to Mothers against decapentaplegic homolog 1 (SMAD 1) (Mothers against DPP homolog 1) (Mad-related protein 1) (Transforming growth factor-beta signaling protein 1) (BSP-1) (hSMAD1) (JV4-1) isoform 13 [Bos taurus] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 1.0E-60
Pfam IPR001132 EMBL-EBI Dwarfin protein 1.1E-113
Sequence
Target Sequence 
>C. ELEGANS:176577_S_AT
gttcttgtacctcgttattccgagaaaccaccacaagaagtgcccccaactctggcgaaa
tttcagttgatggagatgagcggatcaagaatgccgcagaatgtcaatatggcaaacgtt
aattttacagccaaccaatttcatcaatacaatccaaatggaattgaggaaatggatact
tctcagaaattcgacattccaccaggagttccaacatgtctcgtgccattcgataaagtt
tgggaagaacaattctgggcaactgttagctattatgaattgaatacaagagtcggagaa
caggttaaagtgtcttcaacaactataactatcgatggattcactgatccatgcatcaat
ggaagcaaaattagcctgggactgttttcgaatgtaaatcgaaatgcaactattgaaaat
acaagaagacatattggaaacggtgtcaagctaacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCTTGTACCTCGTTATTCCGAGA 167 443 376 Antisense
TCGTTATTCCGAGAAACCACCACAA 582 603 387 Antisense
GAGAAACCACCACAAGAAGTGCCCC 318 391 397 Antisense
TGCCCCCAACTCTGGCGAAATTTCA 541 583 416 Antisense
GGAGATGAGCGGATCAAGAATGCCG 68 519 447 Antisense
AAGAATGCCGCAGAATGTCAATATG 196 151 462 Antisense
GCAGAATGTCAATATGGCAAACGTT 210 311 471 Antisense
TATGGCAAACGTTAATTTTACAGCC 422 655 483 Antisense
TTTTACAGCCAACCAATTTCATCAA 115 675 498 Antisense
AACCAATTTCATCAATACAATCCAA 224 145 508 Antisense
ATTGGAAACGGTGTCAAGCTAACCT 49 3 808 Antisense
Sequence Source Affymetrix Proprietary Database