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[176551_s_at]

Description Y108G3AL.G /REP_DB=WormBase Gene ID /WP=CE26041 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 4grp_high_8_8 > 28      6grp_total > 79      4grp_total > 20      6grp_cgc > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 112.9
(-0.2)
P 		89.2
(-3)
A 		101.3
(-1.6)
M 		140.4
(3)
P 		51
B 110
(0.4)
P 		110
(0.4)
A 		119.4
(3)
M 		97.4
(-3)
P 		22
C 113.4
(-0.2)
P 		131.8
(1.6)
P 		86.1
(-3)
P 		145.4
(3)
P 		59
avr 112.1
(-0.7) 		110.3
(-1.1) 		102.3
(-3) 		127.7
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 37.8
(-3)
P 		79.6
(1)
P 		63.9
(-0.5)
A 		92.4
(2.2)
P 		100.3
(3)
M 		97.7
(2.8)
P 		63
B 51.5
(-3)
A 		88.9
(0.6)
A 		88.1
(0.5)
A 		89.3
(0.6)
A 		91.6
(0.8)
A 		114.4
(3)
P 		63
C 103.4
(0.4)
P 		138.6
(3)
P 		58.8
(-2.8)
A 		56
(-3)
A 		61.4
(-2.6)
P 		105.2
(0.6)
P 		83
avr 64.2
(-3) 		102.4
(2.5) 		70.3
(-2.1) 		79.2
(-0.8) 		84.4
(-0.1) 		105.8
(3) 		42
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 41.1
(3)
P 		19.4
(-3)
A 		26.5
(-1)
M 		20.5
(-2.7)
P 		22
B 35.1
(3)
P 		27.2
(-0.3)
A 		27.7
(-0.1)
M 		20.8
(-3)
P 		14
C 37.5
(2.8)
P 		37.9
(3)
P 		30
(-0.5)
P 		24.5
(-3)
P 		13
avr 37.9
(3) 		28.2
(-0.6) 		28.1
(-0.7) 		21.9
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 7
(-3)
P 		28.3
(2.5)
P 		13.9
(-1.2)
A 		23.4
(1.2)
P 		30.3
(3)
M 		23.8
(1.3)
P 		23
B 17.9
(-3)
A 		29.6
(-0.2)
A 		21.4
(-2.2)
A 		23.6
(-1.6)
A 		37.2
(1.7)
A 		42.7
(3)
P 		25
C 38.3
(2.2)
P 		42.3
(3)
P 		15
(-2.3)
A 		11.3
(-3)
A 		19.9
(-1.3)
P 		35.2
(1.6)
P 		31
avr 21.1
(-1.5) 		33.4
(2.8) 		16.8
(-3) 		19.4
(-2.1) 		29.1
(1.3) 		33.9
(3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176551_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19527
Sequence Type Exemplar sequence
Representative Public ID Y108G3AL.G NCBI
Target Description Y108G3AL.G /REP_DB=WormBase Gene ID /WP=CE26041 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070750(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070750 NCBI Caenorhabditis elegans CULlin family member (cul-3) (cul-3) mRNA, complete cds. 11/11 A 
GENEFINDER00000023664 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:177473:184282:1 11/11 None
Y108G3AL.1.2 ENSEMBL cdna:known chromosome:CEL140:V:177250:184388:1 gene:Y108G3AL.1 11/11 None
Y108G3AL.1.1 ENSEMBL cdna:known chromosome:CEL140:V:177408:184867:1 gene:Y108G3AL.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000023640 7/11 Cross Hyb Matching Probes None
SNAP00000023641 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:173238-184282(+) 94.4 97.12
Public Domain and Genome References
Gene Title
Gene Symbol cul-3
Ensembl Y108G3AL.1
Entrez Gene 178547 Entrez gene
SwissProt Q17391 EMBL-EBI
WormBase CE27593 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:263685_AT cullin, putative at
ATGENOME1:17771_AT cullin, putative at
CANINE:1589793_S_AT similar to Cullin-3 (CUL-3) cfa
CANINE_2:CFA.657.1.A1_S_AT similar to Cullin-3 (CUL-3) cfa
CANINE_2:CFAAFFX.22384.1.S1_S_AT similar to Cullin-3 (CUL-3) cfa
DROSOPHILA_2:1624970_S_AT guftagu dm
DROSGENOME1:153701_AT guftagu dm
CHICKEN:GGA.4769.1.S1_S_AT cullin 3 gga
CHICKEN:GGAAFFX.26407.1.S1_AT cullin 3 gga
CHICKEN:GGAAFFX.26407.3.S1_S_AT cullin 3 gga
HG-U133A:201372_S_AT cullin 3 hs
HG-U133_PLUS_2:201372_S_AT cullin 3 hs
HG-U133A_2:201372_S_AT cullin 3 hs
HG-U133A:201370_S_AT cullin 3 hs
HG-U133_PLUS_2:201370_S_AT cullin 3 hs
HG-U133A_2:201370_S_AT cullin 3 hs
HG-U133A:201371_S_AT cullin 3 hs
HG-U133_PLUS_2:201371_S_AT cullin 3 hs
HG-U133A_2:201371_S_AT cullin 3 hs
HG-FOCUS:201371_S_AT cullin 3 hs
HU35KSUBB:RC_F13623_S_AT cullin 3 hs
HU35KSUBD:RC_H98621_S_AT cullin 3 hs
HUGENEFL:U58089_AT cullin 3 hs
U133_X3P:G4503164_3P_A_AT cullin 3 hs
U133_X3P:G3139078_3P_A_AT cullin 3 hs
U133_X3P:HS.78946.0.A2_3P_A_AT cullin 3 hs
HG-U95AV2:37702_AT cullin 3 hs
HG-U133_PLUS_2:237376_AT Cullin 3 hs
HG-U133B:237376_AT Cullin 3 hs
HG-U133_PLUS_2:237985_AT Cullin 3 hs
HG-U133B:237985_AT Cullin 3 hs
HG-U133_PLUS_2:241016_AT Cullin 3 hs
HG-U133B:241016_AT Cullin 3 hs
HG-U133_PLUS_2:241855_S_AT Cullin 3 hs
HG-U133B:241855_S_AT Cullin 3 hs
HG-U133_PLUS_2:242362_AT Cullin 3 hs
HG-U133B:242362_AT Cullin 3 hs
HG-U95E:69872_AT Cullin 3 hs
HG-U95D:81154_AT Cullin 3 hs
HG-U95D:83329_AT Cullin 3 hs
HG-U95E:83938_AT Cullin 3 hs
HG-U95E:84623_AT Cullin 3 hs
HG-U95D:90657_AT Cullin 3 hs
U133_X3P:HS.155690.0.A1_3P_AT Cullin 3 hs
U133_X3P:HS.155690.1.A1_3P_AT Cullin 3 hs
U133_X3P:HS.159577.0.A1_3P_AT Cullin 3 hs
U133_X3P:HS.171110.0.A1_3P_AT Cullin 3 hs
U133_X3P:HS.201853.0.A1_3P_AT Cullin 3 hs
MG-U74AV2:92797_AT cullin 3 mm
MG-U74BV2:111920_AT cullin 3 mm
MOE430A:1422795_AT cullin 3 mm
MOUSE430A_2:1422795_AT cullin 3 mm
MOUSE430_2:1422795_AT cullin 3 mm
MOE430A:1422794_AT cullin 3 mm
MOUSE430A_2:1422794_AT cullin 3 mm
MOUSE430_2:1422794_AT cullin 3 mm
MOE430A:1450756_S_AT cullin 3 mm
MOUSE430A_2:1450756_S_AT cullin 3 mm
MOUSE430_2:1450756_S_AT cullin 3 mm
MG-U74BV2:108296_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
MG-U74BV2:115360_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
MOUSE430_2:1434717_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
MOE430B:1434717_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
MOE430B:1434718_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
MOUSE430_2:1434718_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
MU19KSUBC:TC40586_AT Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170) mm
RG-U34B:RC_AA901222_AT cullin 3 (predicted) rn
RG-U34C:RC_AI072520_AT cullin 3 (predicted) rn
RAE230A:1399132_AT cullin 3 (predicted) rn
RAT230_2:1399132_AT cullin 3 (predicted) rn
RAE230B:1393343_AT cullin 3 (predicted) rn
RAT230_2:1393343_AT cullin 3 (predicted) rn
RAE230A:1376241_AT Cullin 3 (predicted) rn
RAT230_2:1376241_AT Cullin 3 (predicted) rn
RAE230B:1396400_AT Cullin 3 (predicted) rn
RAT230_2:1396400_AT Cullin 3 (predicted) rn
RG-U34C:RC_AA858761_AT Cullin 3 (predicted) rn
RG-U34C:RC_AI105022_AT Cullin 3 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK72067 Cullin protein 3 [Caenorhabditis elegans] ref|NP_503151.1| CULlin family member (cul-3) [Caenorhabditis elegans] sp|Q17391|CUL3_CAEEL Cullin-3 0.0
blast AAC47122 CUL-3 0.0
blast CAE62355 Hypothetical protein CBG06434 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.SCF ubiquitin ligase complex WHB domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; SCF ubiquitin ligase complex WHB domain 1.00000002374223E-32
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176551_S_AT
tcctggaccgtcgagcagtggagagtcgatgaaagaacgaaaaccggagcacaagattct
ccaggtcaatacgcatcagatgatcattctccttcagttcaaccatcacaatcggatctc
atgtcagcagctgatggatgagcttaagattcccgagcgagagttgaagaggaatttgca
gtcgctggctctcggaaaagcgtcgcagcggattttggtcagaaagaataagggaaaaga
tgcgatagacatgagtgacgagttcgccgtcaacgacaacttccaatcgaagctgacacg
tgtcaaagtgcaaatggtgacaggaaaagtggaatcggagcccgagattcgtgaaactcg
ccagaaagttgaggatgatcgaaagttggaagtggaggcggcgattgttcgtattatgaa
ggcccgaaagaagttgaatcacaataatctggtggcagaggtgacccaacaactgcgcca
ccgcttcatgccgtcaccaatcatcatcaagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTGGACCGTCGAGCAGTGGAGAG 72 593 2655 Antisense
GATTCTCCAGGTCAATACGCATCAG 274 431 2709 Antisense
ATCAGATGATCATTCTCCTTCAGTT 182 29 2729 Antisense
CTCCTTCAGTTCAACCATCACAATC 472 235 2743 Antisense
CAATCGGATCTCATGTCAGCAGCTG 383 181 2763 Antisense
GAAGAGGAATTTGCAGTCGCTGGCT 158 339 2820 Antisense
CTGGCTCTCGGAAAAGCGTCGCAGC 369 241 2839 Antisense
GAGCCCGAGATTCGTGAAACTCGCC 6 387 2992 Antisense
GAAGTGGAGGCGGCGATTGTTCGTA 37 335 3043 Antisense
GCAGAGGTGACCCAACAACTGCGCC 204 311 3109 Antisense
GCCGTCACCAATCATCATCAAGCAA 186 285 3144 Antisense
Sequence Source Affymetrix Proprietary Database