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[176547_at]

Description Y71G12B.FF /REP_DB=WormBase Gene ID /WP=CE22909 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 88      4grp_total > 12      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 56.9
(2.2)
A 		8.6
(-3)
A 		8.5
(-3)
A 		64.6
(3)
A 		56
B 20.3
(-2.7)
A 		25.9
(-2.1)
A 		17
(-3)
A 		76
(3)
P 		59
C 8.8
(-2.6)
A 		14.6
(-1.8)
A 		6.1
(-3)
A 		48.8
(3)
A 		43
avr 28.7
(-0.9) 		16.4
(-2.3) 		10.5
(-3) 		63.1
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 39
(-1.5)
A 		1.7
(-3)
A 		31
(-1.8)
A 		39.5
(-1.4)
A 		87.6
(0.5)
P 		147.9
(3)
P 		146
B 11.6
(-3)
A 		38.8
(-1.9)
A 		20.2
(-2.6)
A 		17.4
(-2.8)
A 		96.9
(0.6)
P 		155.4
(3)
P 		144
C 14.5
(-2.6)
A 		54.9
(-1.2)
A 		4.6
(-3)
A 		39
(-1.7)
A 		84.7
(-0.1)
P 		169.1
(3)
P 		165
avr 21.7
(-2.9) 		31.8
(-2.4) 		18.6
(-3) 		32
(-2.4) 		89.7
(0.1) 		157.5
(3) 		139
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 20.7
(3)
A 		1.9
(-3)
A 		2.2
(-2.9)
A 		9.5
(-0.6)
A 		19
B 6.5
(-1.7)
A 		6.4
(-1.8)
A 		3.9
(-3)
A 		16.2
(3)
P 		12
C 2.9
(-2.2)
A 		4.2
(-0.9)
A 		2.1
(-3)
A 		8.2
(3)
A 		6
avr 10
(2.1) 		4.2
(-2) 		2.7
(-3) 		11.3
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 7.2
(-1.9)
A 		0.6
(-3)
A 		6.7
(-2)
A 		10
(-1.4)
A 		26.4
(1.4)
P 		36
(3)
P 		35
B 4
(-3)
A 		12.9
(-2)
A 		4.9
(-2.9)
A 		4.6
(-2.9)
A 		39.4
(0.9)
P 		57.9
(3)
P 		54
C 5.4
(-2.5)
A 		16.7
(-1.3)
A 		1.2
(-3)
A 		7.8
(-2.3)
A 		27.4
(-0.2)
P 		56.6
(3)
P 		55
avr 5.5
(-2.8) 		10.1
(-2.2) 		4.3
(-3) 		7.5
(-2.6) 		31.1
(0.5) 		50.2
(3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176547_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18648
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.FF NCBI
Target Description Y71G12B.FF /REP_DB=WormBase Gene ID /WP=CE22909 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058498(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058498 NCBI Caenorhabditis elegans Y71G12B.3 (Y71G12B.3) mRNA, complete cds. 11/11 None
SNAP00000001800 ENSEMBL cdna:SNAP chromosome:CEL140:I:1810569:1817031:1 9/11 None
GENEFINDER00000001820 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1812956:1813538:1 11/11 None
Y71G12B.3 ENSEMBL cdna:known chromosome:CEL140:I:1812956:1813538:1 gene:Y71G12B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1812955-1813538(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71G12B.3
Entrez Gene 190597 Entrez gene
SwissProt Q95XM1 EMBL-EBI
WormBase CE22909 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73930 Hypothetical protein Y71G12B.4 [Caenorhabditis elegans] ref|NP_490898.1| Y71G12B.4 [Caenorhabditis elegans] 1.0E-118
blast CAE74457 Hypothetical protein CBG22199 [Caenorhabditis briggsae] 1.0E-107
blast AAK73929 Hypothetical protein Y71G12B.3 [Caenorhabditis elegans] ref|NP_490899.1| Y71G12B.3 [Caenorhabditis elegans] 6.0E-71
blast AAO92288 peptidylglycine alpha-hydroxylating monooxygenase [Heterodera glycines] 3.0E-63
blast CAB03885 Hypothetical protein C14A6.8 [Caenorhabditis elegans] ref|NP_507551.1| C14A6.8 [Caenorhabditis elegans] 1.0E-23
blast CAA16326 Hypothetical protein Y39E4A.1 [Caenorhabditis elegans] ref|NP_499690.1| Y39E4A.1 [Caenorhabditis elegans] 5.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 6.6E-8
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 9.3E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490899.1 1 147-169
Y71G12B.3 1 147-169
GENEFINDER00000001820 1 147-169
Sequence
Target Sequence 
>C. ELEGANS:176547_AT
tctttgatccaactgtcccagtcaaaaatgatccgctgaaatccggtggcgatgagtatc
acaatctcggtgcatttgactacgcgaatctggatccaaatgcgccgccagtcggaggaa
atgaagtgccaccaccaccgccgccggagacagttgctcacgatgcacttatttcggatg
atggagcagcaaaagtggcaactggtgatggagttcaggagttgcccgagtcggatccat
cgccatccaagaagccaagagtcaataaggcgaaagtcaagaagttgaagaacaagatcg
tgagttttcgaagttggcgcccagtgtgcgccgcaagcggcgccggcggcggcgcaatcg
aaagtaagccaccgccggcgcacggcgctcaggctgtcacagtgtgccgtcttataaact
tgaaagtcttcttctggacaatgatcggaatttgggttatttgcatgtgtctcttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTTGATCCAACTGTCCCAGTCAA 394 613 26 Antisense
ATCACAATCTCGGTGCATTTGACTA 594 27 83 Antisense
ATTTGACTACGCGAATCTGGATCCA 37 19 99 Antisense
CGCCGGAGACAGTTGCTCACGATGC 459 257 167 Antisense
GCTCACGATGCACTTATTTCGGATG 378 301 181 Antisense
TCGCCATCCAAGAAGCCAAGAGTCA 527 599 265 Antisense
GTGAGTTTTCGAAGTTGGCGCCCAG 120 481 325 Antisense
GCCCAGTGTGCGCCGCAAGCGGCGC 283 281 344 Antisense
GCAATCGAAAGTAAGCCACCGCCGG 32 321 379 Antisense
TGTCACAGTGTGCCGTCTTATAAAC 138 559 420 Antisense
TGGGTTATTTGCATGTGTCTCTTCG 665 553 478 Antisense
Sequence Source Affymetrix Proprietary Database