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[176544_at]

Description Y71G12B.EE /REP_DB=WormBase Gene ID /WP=CE26253 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 46      4grp_high_8_8 > 11      6grp_highest_nonorm > 15      6grp_total > 24      4grp_total > 14      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8082
1 2 3 4 range
A 7081.6
(0.9)
P 		3175.2
(-2.4)
P 		2434.1
(-3)
P 		9650.4
(3)
P 		7216
B 7152
(0.5)
P 		3241.6
(-2.5)
P 		2528.7
(-3)
P 		10377
(3)
P 		7848
C 7001.8
(1.5)
P 		2839.7
(-2.5)
P 		2295.5
(-3)
P 		8504.9
(3)
P 		6209
avr 7078.5
(0.9) 		3085.5
(-2.4) 		2419.4
(-3) 		9510.8
(3) 		7091

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3273
1 2 3 4 5 6 range
A 6727.5
(3)
P 		4835.5
(-0.9)
P 		4919.3
(-0.8)
P 		4015.2
(-2.7)
P 		3960.6
(-2.8)
P 		3848.5
(-3)
P 		2879
B 6078
(2.5)
P 		5884.7
(1.9)
P 		6251.2
(3)
P 		5172.1
(-0.4)
P 		4433.9
(-2.7)
P 		4322.7
(-3)
P 		1929
C 5394.2
(1.4)
P 		6077.1
(3)
P 		4795.2
(0.1)
P 		5185
(1)
P 		3454.8
(-3)
P 		4573.4
(-0.4)
P 		2622
avr 6066.6
(3) 		5599.1
(1.7) 		5321.9
(0.9) 		4790.8
(-0.6) 		3949.8
(-3) 		4248.2
(-2.2) 		2117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1992
1 2 3 4 range
A 2578.4
(3)
P 		689.3
(-2.8)
P 		636.3
(-3)
P 		1412.6
(-0.6)
P 		1942
B 2280.7
(3)
P 		800.6
(-2.2)
P 		586
(-3)
P 		2211.3
(2.8)
P 		1695
C 2317.8
(3)
P 		816.4
(-2.9)
P 		800.1
(-3)
P 		1434.1
(-0.5)
P 		1518
avr 2392.3
(3) 		768.8
(-2.7) 		674.1
(-3) 		1686
(0.5) 		1718

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1178
1 2 3 4 5 6 range
A 1240.2
(-0.7)
P 		1719.8
(3)
P 		1071.3
(-2)
P 		1018.3
(-2.4)
P 		1195.1
(-1)
P 		937
(-3)
P 		783
B 2114.9
(3)
P 		1958.8
(1.7)
P 		1518
(-1.8)
P 		1369.9
(-3)
P 		1801.4
(0.5)
P 		1611.9
(-1.1)
P 		745
C 1999.4
(3)
P 		1854.3
(2.1)
P 		1220.5
(-1.9)
P 		1042.7
(-3)
P 		1117.9
(-2.5)
P 		1531
(0.1)
P 		957
avr 1784.8
(2.5) 		1844.3
(3) 		1269.9
(-1.9) 		1143.6
(-3) 		1371.5
(-1) 		1360
(-1.1) 		701
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176544_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18762
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.EE NCBI
Target Description Y71G12B.EE /REP_DB=WormBase Gene ID /WP=CE26253 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058497(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058497 NCBI Caenorhabditis elegans Y71G12B.4 (Y71G12B.4) mRNA, complete cds. 11/11 None
GENEFINDER00000001827 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1808120:1812853:1 11/11 None
SNAP00000001800 ENSEMBL cdna:SNAP chromosome:CEL140:I:1810569:1817031:1 10/11 None
Y71G12B.4 ENSEMBL cdna:known chromosome:CEL140:I:1808094:1811766:1 gene:Y71G12B.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1808119-1811670(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71G12B.4
Entrez Gene 171746 Entrez gene
SwissProt Q95XM2 EMBL-EBI
WormBase CE26253 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1631675_A_AT Peptidylglycine-hydroxylating monooxygenase dm
DROSOPHILA_2:1623430_AT Peptidylglycine-hydroxylating monooxygenase dm
DROSGENOME1:152428_AT Peptidylglycine-hydroxylating monooxygenase dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6518 peptide metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
4504 peptidylglycine monooxygenase activity inferred from electronic annotation QuickGO AmiGO
5507 copper ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK73930 Hypothetical protein Y71G12B.4 [Caenorhabditis elegans] ref|NP_490898.1| Y71G12B.4 [Caenorhabditis elegans] 0.0
blast CAE74457 Hypothetical protein CBG22199 [Caenorhabditis briggsae] 1.0E-169
blast AAK73930 Hypothetical protein Y71G12B.4 [Caenorhabditis elegans] ref|NP_490898.1| Y71G12B.4 [Caenorhabditis elegans] 1.0E-118
blast CAE74457 Hypothetical protein CBG22199 [Caenorhabditis briggsae] 1.0E-107
blast AAO92288 peptidylglycine alpha-hydroxylating monooxygenase [Heterodera glycines] 1.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 3.5E-50
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 9.3E-38
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 6.6E-8
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 9.3E-38
Sequence
Target Sequence 
>C. ELEGANS:176544_AT
caagatgttggcttccgtgttggaggagacagcaacatccgtcaccttgtcatgcaagtc
cattacatgcactcaaaacaggaacccgatgagacaggcttagagattacccacaccgaa
gaaccccaaccaaagctcgccgccaccatgctcctggtcaccggaggaactctgccacgt
aacaagacagaatccttcgagactgcttgcatgattgaggaagacgtcgtcatgcaccca
ttcgcctaccgtacccatactcacagacatggaaaggaggtcagcgggtggttggtgaag
gaggatcaaaagcacgaggaccactggaagctcatcggacggagggatcctcagcttgct
cagatgtttgtgccagttgaagatcaggcgatgacaatccagcagggagatatggtgact
gcacggtgtattctgcagaacaacgagaatagagacatctcgatgggagcaactgaggaa
gacgaaatgtgcaatttctatattatgtactggactgatggagaagtgatgcaggacaac
acttgttattcgcctggagcaccggattacaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGATGTTGGCTTCCGTGTTGGAG 105 185 376 Antisense
GGAGGAGACAGCAACATCCGTCACC 468 515 397 Antisense
TCCGTCACCTTGTCATGCAAGTCCA 41 595 413 Antisense
CAGGCTTAGAGATTACCCACACCGA 400 209 470 Antisense
AATCCTTCGAGACTGCTTGCATGAT 548 167 566 Antisense
AGGAAGACGTCGTCATGCACCCATT 93 55 593 Antisense
GCACGAGGACCACTGGAAGCTCATC 699 315 687 Antisense
AGCTCATCGGACGGAGGGATCCTCA 166 79 704 Antisense
AGCTTGCTCAGATGTTTGTGCCAGT 215 79 728 Antisense
GGACAACACTTGTTATTCGCCTGGA 190 527 909 Antisense
TTCGCCTGGAGCACCGGATTACAAA 579 691 924 Antisense
Sequence Source Affymetrix Proprietary Database