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[176523_at]

Description Y46E12BL.B /REP_DB=WormBase Gene ID /WP=CE26536 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 0      4grp_total > 19      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 44.4
(-1.8)
A 		87.5
(3)
A 		34
(-3)
A 		76.9
(1.8)
A 		54
B 39.2
(-2.2)
A 		35.7
(-2.8)
A 		34.4
(-3)
A 		72.1
(3)
A 		38
C 44.3
(0.4)
A 		33.8
(-0.7)
A 		11.9
(-3)
A 		69.5
(3)
A 		58
avr 42.6
(-0.9) 		52.3
(0.3) 		26.8
(-3) 		72.8
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 8.4
(-3)
A 		18.7
(-2.1)
A 		24.5
(-1.6)
A 		13.1
(-2.6)
A 		76.1
(3)
A 		8.4
(-3)
A 		68
B 21.9
(-0.6)
A 		24
(-0.4)
A 		49.5
(3)
A 		19.5
(-1)
A 		4.1
(-3)
A 		43.9
(2.3)
A 		45
C 5.3
(-3)
A 		64.8
(3)
A 		5.7
(-3)
A 		13
(-2.2)
A 		36.1
(0.1)
A 		48
(1.3)
A 		60
avr 11.9
(-3) 		35.8
(2.3) 		26.6
(0.3) 		15.2
(-2.3) 		38.8
(3) 		33.4
(1.8) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 16.2
(1.3)
A 		19
(3)
A 		8.9
(-3)
A 		11.3
(-1.6)
A 		10
B 12.5
(0.6)
A 		8.8
(-2.4)
A 		8
(-3)
A 		15.4
(3)
A 		7
C 14.7
(3)
A 		9.7
(0.2)
A 		4.1
(-3)
A 		11.7
(1.3)
A 		11
avr 14.5
(3) 		12.5
(1.4) 		7
(-3) 		12.8
(1.6) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 1.5
(-3)
A 		6.7
(-1.5)
A 		5.3
(-1.9)
A 		3.3
(-2.5)
A 		23
(3)
A 		2
(-2.9)
A 		22
B 7.6
(-0.6)
A 		8
(-0.4)
A 		12
(1.2)
A 		5.2
(-1.6)
A 		1.7
(-3)
A 		16.4
(3)
A 		15
C 2
(-2.8)
A 		19.8
(3)
A 		1.4
(-3)
A 		2.6
(-2.6)
A 		11.7
(0.4)
A 		16.1
(1.8)
A 		18
avr 3.7
(-3) 		11.5
(2.6) 		6.2
(-1.2) 		3.7
(-3) 		12.1
(3) 		11.5
(2.6) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176523_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19027
Sequence Type Exemplar sequence
Representative Public ID Y46E12BL.B NCBI
Target Description Y46E12BL.B /REP_DB=WormBase Gene ID /WP=CE26536 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064920(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064920 NCBI Caenorhabditis elegans Y46E12BL.4 (Y46E12BL.4) mRNA, complete cds. 11/11 None
SNAP00000018034 ENSEMBL cdna:SNAP chromosome:CEL140:II:15219462:15226618:1 11/11 None
GENEFINDER00000018043 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15225941:15226618:1 11/11 None
Y46E12BL.4 ENSEMBL cdna:known chromosome:CEL140:II:15222730:15226618:1 gene:Y46E12BL.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15222915-15226615(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y46E12BL.4
Entrez Gene 266876 Entrez gene
WormBase CE39050 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68491 Hypothetical protein Y46E12BL.4 [Caenorhabditis elegans] ref|NP_497321.1| Y46E12BL.4 [Caenorhabditis elegans] 0.0
blast AAK68491 Hypothetical protein Y46E12BL.4 [Caenorhabditis elegans] ref|NP_497321.1| Y46E12BL.4 [Caenorhabditis elegans] 1.0E-173
blast CAE73242 Hypothetical protein CBG20654 [Caenorhabditis briggsae] 1.0E-123
blast CAE73242 Hypothetical protein CBG20654 [Caenorhabditis briggsae] 3.0E-99
blast AAK68491 Hypothetical protein Y46E12BL.4 [Caenorhabditis elegans] ref|NP_497321.1| Y46E12BL.4 [Caenorhabditis elegans] 3.0E-76
blast XP_780123 PREDICTED: similar to CG2944-PA, isoform A isoform 1 [Strongylocentrotus purpuratus] 1.0E-71
blast XP_780123 PREDICTED: similar to CG2944-PA, isoform A isoform 1 [Strongylocentrotus purpuratus] 5.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 2.8E-18
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 1.3E-7
Sequence
Target Sequence 
>C. ELEGANS:176523_AT
gttatacccaattgtggcggccgtttggggtcactgcgagattagtatgagatatctggg
atcactggaacgtgagctaatttctttcgattcttcttcacccactccaccgcgacgcct
tccaccactgagaccaccaccgtcttctcttcagcatcaccaccaccaccgccggtcccc
tccgctctttcattctcctgtctataagcctaagcctagaagtcggactgaatcgccggc
ggccttaaaagcggcagcagcagcggcctcccaatccccagttcttccgttctcgccgtt
ttccgatctcgctaagattttctcgttttacgatcttcaagctggcggggcgtccagctc
gtccagctcgtactctctttttgaaccgcctgccgaatggcacaaatttgatagggagga
cttgccgattgatgagaccccatcggcttcttcacccaccaccaccgcatccctatatct
tccggctccaccatcatcatcggttcgagccgcgaatgagctcttcggacctccgttgac
accgggagattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTATACCCAATTGTGGCGGCCGTT 55 447 405 Antisense
CCGTTTGGGGTCACTGCGAGATTAG 247 261 425 Antisense
GTGAGCTAATTTCTTTCGATTCTTC 575 481 476 Antisense
TTCATTCTCCTGTCTATAAGCCTAA 427 689 593 Antisense
AGAAGTCGGACTGAATCGCCGGCGG 634 73 622 Antisense
CTCGTTTTACGATCTTCAAGCTGGC 615 231 726 Antisense
TTGAACCGCCTGCCGAATGGCACAA 191 703 785 Antisense
AGGACTTGCCGATTGATGAGACCCC 587 55 821 Antisense
CACCATCATCATCGGTTCGAGCCGC 523 201 893 Antisense
GAGCCGCGAATGAGCTCTTCGGACC 666 385 911 Antisense
GACCTCCGTTGACACCGGGAGATTC 324 371 932 Antisense
Sequence Source Affymetrix Proprietary Database