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[176518_at]

Description Y43B11AR.A /REP_DB=WormBase Gene ID /WP=CE26101 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 12      6grp_total > 15      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1538
1 2 3 4 range
A 1917.1
(3)
P 		1512
(1.4)
P 		974.8
(-0.7)
P 		383.9
(-3)
P 		1533
B 1779.1
(3)
P 		1509
(1.8)
P 		1065.1
(-0.1)
P 		379.3
(-3)
P 		1400
C 1896.8
(3)
P 		1615.1
(1.9)
P 		1030.8
(-0.4)
P 		381.6
(-3)
P 		1515
avr 1864.3
(3) 		1545.4
(1.7) 		1023.6
(-0.4) 		381.6
(-3) 		1483

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2004
1 2 3 4 5 6 range
A 2711.9
(2.9)
P 		2742.2
(3)
P 		1069.6
(-2.7)
P 		1363.2
(-1.7)
P 		1491.2
(-1.3)
P 		980.5
(-3)
P 		1762
B 2715.3
(3)
P 		2570.4
(2.5)
P 		1626.5
(-0.4)
P 		1682.3
(-0.2)
P 		1014.2
(-2.3)
P 		790.3
(-3)
P 		1925
C 2787.1
(3)
P 		2586.2
(2.4)
P 		1838.7
(0.2)
P 		1290.7
(-1.5)
P 		1476.3
(-0.9)
P 		783.5
(-3)
P 		2004
avr 2738.1
(3) 		2632.9
(2.7) 		1511.6
(-0.9) 		1445.4
(-1.1) 		1327.2
(-1.5) 		851.4
(-3) 		1887
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 642
1 2 3 4 range
A 698
(3)
P 		328.2
(-0.5)
P 		254.8
(-1.1)
P 		56.2
(-3)
P 		642
B 567.3
(3)
P 		372.7
(0.6)
P 		246.8
(-1)
P 		80.8
(-3)
P 		487
C 627.9
(3)
P 		464.4
(1.3)
P 		359.3
(0.1)
P 		64.3
(-3)
P 		564
avr 631.1
(3) 		388.4
(0.4) 		287
(-0.7) 		67.1
(-3) 		564

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 800
1 2 3 4 5 6 range
A 499.9
(-0.8)
P 		975.3
(3)
P 		232.9
(-3)
P 		345.7
(-2.1)
P 		450
(-1.2)
P 		238.7
(-3)
P 		742
B 944.8
(3)
P 		855.6
(2.2)
P 		395
(-2.1)
P 		445.6
(-1.6)
P 		412.1
(-1.9)
P 		294.7
(-3)
P 		650
C 1033
(3)
P 		789.1
(1.1)
P 		468
(-1.4)
P 		259.6
(-3)
P 		477.7
(-1.3)
P 		262.3
(-3)
P 		773
avr 825.9
(2.5) 		873.3
(3) 		365.3
(-2) 		350.3
(-2.2) 		446.6
(-1.2) 		265.2
(-3) 		608
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176518_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19107
Sequence Type Exemplar sequence
Representative Public ID Y43B11AR.A NCBI
Target Description Y43B11AR.A /REP_DB=WormBase Gene ID /WP=CE26101 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068703, NM_171374, NM_068704
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000039668 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7085672:7094250:-1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068704 8/11 Cross Hyb Matching Probes None
NM_171374 7/11 Cross Hyb Matching Probes None
NM_068703 8/11 Cross Hyb Matching Probes None
SNAP00000039643 7/11 Cross Hyb Matching Probes None
ZC477.9b 8/11 Cross Hyb Matching Probes A 
ZC477.9c 7/11 Cross Hyb Matching Probes A 
ZC477.9a 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7085677-7086435(-) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM81104 Dense body protein 1, isoform a [Caenorhabditis elegans] ref|NP_501104.2| DEnse Body family member (deb-1) [Caenorhabditis elegans] sp|P19826|VINC_CAEEL Vinculin (P107B) gb|AAA28002.1| vinculin 0.0
blast AAM81106 Dense body protein 1, isoform c [Caenorhabditis elegans] ref|NP_741437.1| DEnse Body family member (deb-1) [Caenorhabditis elegans] 0.0
blast Q17162 Vinculin gb|AAB96842.1| vinculin [Brugia malayi] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 1.2E-7
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176518_AT
ggatctcatcaattgctccaaggcaatcgcagattcttctgaagaagttaccagattggc
agttcaacttgccagactttgcacagatatcaagatgagaacggcactccttcaagtctc
agaaagaattccaacgatagctacacaattgaaggtcttgtcaacagtcaaggcgacaat
gctcggatcagccaaacccatttcagatgtcatcggcccatacggtcaaccagtcgaggg
cagcgaagaagatgatgaagcaatgcaacaattggtgcataatgctcaaaacttgatgca
atctgtgaaggatgttgtccgtgctgctgaagccgcctctattaaaatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCTCATCAATTGCTCCAAGGCA 708 511 29 Antisense
GCTCCAAGGCAATCGCAGATTCTTC 2 299 43 Antisense
GTTCAACTTGCCAGACTTTGCACAG 550 445 90 Antisense
AGATGAGAACGGCACTCCTTCAAGT 356 67 121 Antisense
GGCACTCCTTCAAGTCTCAGAAAGA 690 535 131 Antisense
AAGGCGACAATGCTCGGATCAGCCA 135 161 198 Antisense
GGATCAGCCAAACCCATTTCAGATG 388 511 213 Antisense
TTTCAGATGTCATCGGCCCATACGG 282 671 229 Antisense
GCCCATACGGTCAACCAGTCGAGGG 55 285 244 Antisense
TGAAGGATGTTGTCCGTGCTGCTGA 610 575 334 Antisense
GCTGAAGCCGCCTCTATTAAAATCC 304 253 354 Antisense
Sequence Source Affymetrix Proprietary Database