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[176517_at]

Description Y71G12B.GG /REP_DB=WormBase Gene ID /WP=CE22910 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 19      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 61.6
(-0.1)
A 		46.1
(-1)
A 		119.5
(3)
A 		8.2
(-3)
A 		111
B 62
(1.6)
A 		76.8
(3)
A 		37.9
(-0.6)
A 		11.1
(-3)
A 		66
C 23.8
(-3)
A 		39.9
(-1.7)
A 		65.9
(0.4)
A 		98.3
(3)
A 		75
avr 49.1
(-1.3) 		54.3
(-0.4) 		74.4
(3) 		39.2
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 83.5
(1.5)
A 		9.8
(-3)
A 		45.7
(-0.8)
A 		57.3
(-0.1)
A 		108.4
(3)
A 		60.4
(0.1)
A 		99
B 68
(3)
A 		51.6
(1.3)
A 		18.3
(-2.2)
A 		10.6
(-3)
A 		10.2
(-3)
A 		28.2
(-1.1)
A 		58
C 11.5
(-2.7)
A 		22.3
(-1.5)
A 		65.8
(3)
A 		20.1
(-1.8)
A 		8.3
(-3)
A 		22.8
(-1.5)
A 		58
avr 54.3
(3) 		27.9
(-3) 		43.3
(0.5) 		29.3
(-2.7) 		42.3
(0.3) 		37.1
(-0.9) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 22.4
(1.2)
A 		10
(-1.2)
A 		31.2
(3)
A 		1.2
(-3)
A 		30
B 19.8
(3)
A 		19
(2.7)
A 		8.8
(-0.8)
A 		2.4
(-3)
A 		17
C 7.9
(-3)
A 		11.5
(-1.6)
A 		23
(3)
A 		16.6
(0.5)
A 		15
avr 16.7
(1.2) 		13.5
(-0.1) 		21
(3) 		6.7
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 15.4
(-0.6)
A 		3.5
(-3)
A 		10
(-1.7)
A 		14.5
(-0.7)
A 		32.7
(3)
A 		14.7
(-0.7)
A 		29
B 23.7
(3)
A 		17.2
(1.1)
A 		4.5
(-2.5)
A 		2.8
(-3)
A 		4.2
(-2.6)
A 		10.5
(-0.8)
A 		21
C 4.3
(-2.3)
A 		6.8
(-1.2)
A 		16.7
(3)
A 		4
(-2.4)
A 		2.7
(-3)
A 		7.6
(-0.9)
A 		14
avr 14.5
(3) 		9.2
(-1.3) 		10.4
(-0.3) 		7.1
(-3) 		13.2
(1.9) 		10.9
(0.1) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176517_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18712
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.GG NCBI
Target Description Y71G12B.GG /REP_DB=WormBase Gene ID /WP=CE22910 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058499
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058499 NCBI Caenorhabditis elegans Y71G12B.2 (Y71G12B.2) mRNA, complete cds. None
SNAP00000001800 ENSEMBL cdna:SNAP chromosome:CEL140:I:1810569:1817031:1 None
Y71G12B.2 ENSEMBL cdna:known chromosome:CEL140:I:1815071:1817658:1 gene:Y71G12B.2 None
SNAP00000001801 ENSEMBL cdna:SNAP chromosome:CEL140:I:1817308:1818119:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1814492-1819250(+) 81.02 98.59
Public Domain and Genome References
Gene Title
Ensembl Y71G12B.2
Entrez Gene 190596 Entrez gene
SwissProt Q4W5R2 EMBL-EBI
WormBase CE38651 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAY43985 Hypothetical protein Y71G12B.2 [Caenorhabditis elegans] ref|NP_490900.2| Y71G12B.2 [Caenorhabditis elegans] 1.0E-145
blast AAK73930 Hypothetical protein Y71G12B.4 [Caenorhabditis elegans] ref|NP_490898.1| Y71G12B.4 [Caenorhabditis elegans] 1.0E-118
blast CAE74457 Hypothetical protein CBG22199 [Caenorhabditis briggsae] 1.0E-107
blast AAY43985 Hypothetical protein Y71G12B.2 [Caenorhabditis elegans] ref|NP_490900.2| Y71G12B.2 [Caenorhabditis elegans] 1.0E-103
blast AAO92288 peptidylglycine alpha-hydroxylating monooxygenase [Heterodera glycines] 3.0E-63
blast AAY43985 Hypothetical protein Y71G12B.2 [Caenorhabditis elegans] ref|NP_490900.2| Y71G12B.2 [Caenorhabditis elegans] 1.0E-36
blast CAA92443 Hypothetical protein C10C5.2 [Caenorhabditis elegans] ref|NP_501649.1| C10C5.2 [Caenorhabditis elegans] 7.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 6.6E-8
Pfam IPR000323 EMBL-EBI Copper type II, ascorbate-dependent monooxygenase 9.3E-38
Sequence
Target Sequence 
>C. ELEGANS:176517_AT
tgctccaacagccacttgtgctggagcaggagccttcttgattggcgcggccgataggtc
atgtccaaacgtattgatcgtgcattcctggaatagtaatttgaatggatactatcgtgg
tgagacccacgccaccagtttcctctacgcctacttgcctgcctgcctacgtgcctactt
aagtagctcccactgcctacctacacacgtggtgccaggctatcccatcatggtttgctc
tacgaaaaatgcgggaatttttggtccaagaaaatgggacatcaacgtagatcaagccga
aataagagacactctagcctcaattagacccgtttttgctcaccatattggtagccactt
gcaacactgctcccagattatcaaagtgaccacaataacacggcgccgagaagattgtca
cgagcttcttgtcggcaaaaaactcgtatccatccatgacgacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCCAACAGCCACTTGTGCTGGA 534 581 924 Antisense
AGCAGGAGCCTTCTTGATTGGCGCG 268 77 948 Antisense
GATTGGCGCGGCCGATAGGTCATGT 316 435 963 Antisense
GTGCCTACTTAAGTAGCTCCCACTG 668 477 1094 Antisense
GGCTATCCCATCATGGTTTGCTCTA 601 537 1141 Antisense
CACTCTAGCCTCAATTAGACCCGTT 213 197 1233 Antisense
ACCCGTTTTTGCTCACCATATTGGT 528 89 1251 Antisense
TATTGGTAGCCACTTGCAACACTGC 434 661 1269 Antisense
GCAACACTGCTCCCAGATTATCAAA 592 319 1284 Antisense
CACGGCGCCGAGAAGATTGTCACGA 337 199 1322 Antisense
AACTCGTATCCATCCATGACGACCT 619 141 1364 Antisense
Sequence Source Affymetrix Proprietary Database