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[176501_at]

Description Y54F10AM.D /REP_DB=WormBase Gene ID /WP=CE26172 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 4grp_high_8_8 > 11      6grp_total > 25      4grp_total > 31      6grp_cgc > 33      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 56.9
(1.2)
P 		36.1
(-3)
P 		65.5
(3)
M 		64.1
(2.7)
P 		29
B 82.8
(3)
P 		77.5
(2.4)
P 		62.1
(0.7)
P 		28.7
(-3)
A 		54
C 51.5
(-1.4)
P 		52.1
(-1.3)
P 		38.6
(-3)
P 		85.6
(3)
P 		47
avr 63.7
(3) 		55.2
(-3) 		55.4
(-2.9) 		59.5
(0) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 36
(0.2)
A 		54.9
(3)
P 		25
(-1.5)
A 		26.4
(-1.3)
A 		15
(-3)
A 		22.4
(-1.9)
A 		40
B 19.3
(0.2)
P 		22.8
(1)
A 		12.2
(-1.3)
A 		8.4
(-2.1)
A 		32.3
(3)
A 		4
(-3)
A 		28
C 59.1
(3)
P 		49.4
(1.8)
A 		51.3
(2)
P 		10.7
(-3)
A 		12.9
(-2.7)
A 		17.6
(-2.1)
A 		48
avr 38.1
(2.1) 		42.4
(3) 		29.5
(0.2) 		15.2
(-2.9) 		20.1
(-1.8) 		14.7
(-3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 20.7
(3)
P 		7.8
(-3)
P 		17.1
(1.3)
M 		9.4
(-2.3)
P 		13
B 26.4
(3)
P 		19.1
(0.8)
P 		14.4
(-0.5)
P 		6.1
(-3)
A 		20
C 17
(3)
P 		15
(-0.3)
P 		13.4
(-3)
P 		14.4
(-1.3)
P 		4
avr 21.4
(3) 		14
(-0.9) 		15
(-0.4) 		10
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 6.6
(-2.2)
A 		19.5
(3)
P 		5.4
(-2.6)
A 		6.7
(-2.1)
A 		4.5
(-3)
A 		5.5
(-2.6)
A 		15
B 6.7
(-0.3)
P 		7.6
(0.2)
A 		3
(-2.2)
A 		2.2
(-2.6)
A 		13.1
(3)
A 		1.5
(-3)
A 		12
C 21.9
(3)
P 		15.1
(0.9)
A 		13.1
(0.3)
P 		2.1
(-3)
A 		4.2
(-2.4)
A 		5.9
(-1.8)
A 		20
avr 11.7
(1.6) 		14.1
(3) 		7.2
(-1) 		3.7
(-3) 		7.3
(-0.9) 		4.3
(-2.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176501_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18939
Sequence Type Exemplar sequence
Representative Public ID Y54F10AM.D NCBI
Target Description Y54F10AM.D /REP_DB=WormBase Gene ID /WP=CE26172 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065172(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065172 NCBI Caenorhabditis elegans RaB Connectin related family member (rbc-2) (rbc-2) mRNA, complete cds. 11/11 None
SNAP00000034921 ENSEMBL cdna:SNAP chromosome:CEL140:III:2509485:2528616:-1 10/11 None
Y54F10AM.10 ENSEMBL cdna:known chromosome:CEL140:III:2512636:2528616:-1 gene:Y54F10AM.10 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000034932 4/11 Cross Hyb Matching Probes None
GENEFINDER00000034930 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2512635-2515283(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rbc-2
Ensembl Y54F10AM.10
Entrez Gene 190267 Entrez gene
SwissProt Q9BL05 EMBL-EBI
WormBase CE35950 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597274_AT similar to WD repeat domain 7 protein isoform 2 cfa
CANINE_2:CFAAFFX.1176.1.S1_S_AT similar to WD repeat domain 7 protein isoform 2 cfa
DROSOPHILA_2:1636006_AT dm
DROSGENOME1:143990_AT dm
CHICKEN:GGAAFFX.25716.1.S1_AT similar to WD repeat domain 7 protein isoform 2; TGF-beta resistance associated gene; rabconnectin-3 beta gga
HU35KSUBB:RC_F10381_S_AT WD repeat domain 7 hs
U133_X3P:HS.10881.0.S1_3P_AT WD repeat domain 7 hs
HG-U133A_2:212880_AT WD repeat domain 7 hs
HG-U133_PLUS_2:212880_AT WD repeat domain 7 hs
HG-U133A:212880_AT WD repeat domain 7 hs
HG-U95AV2:41430_AT WD repeat domain 7 hs
HG-U133_PLUS_2:241264_AT WD repeat domain 7 hs
HG-U133B:241264_AT WD repeat domain 7 hs
HG-U95C:56396_AT WD repeat domain 7 hs
HG-U95D:69252_I_AT WD repeat domain 7 hs
HG-U95D:69254_R_AT WD repeat domain 7 hs
HG-U95E:80292_AT WD repeat domain 7 hs
HG-U95D:84683_AT WD repeat domain 7 hs
U133_X3P:HS.164277.0.A1_3P_AT WD repeat domain 7 hs
HU35KSUBB:RC_AA218543_AT WD repeat domain 7 hs
HU35KSUBB:RC_T64012_AT WD repeat domain 7 hs
MG-U74BV2:113331_AT WD repeat domain 7 mm
MOE430B:1435028_AT WD repeat domain 7 mm
MOUSE430_2:1435028_AT WD repeat domain 7 mm
MOE430B:1441775_AT MKIAA0541 protein mm
MOUSE430_2:1441775_AT MKIAA0541 protein mm
MG-U74CV2:132607_AT MKIAA0541 protein mm
RAE230A:1369344_AT WD repeat domain 7 rn
RAT230_2:1369344_AT WD repeat domain 7 rn
RAE230A:1372833_AT WD repeat domain 7 rn
RAT230_2:1372833_AT WD repeat domain 7 rn
RAE230B:1397017_AT WD repeat domain 7 rn
RAT230_2:1397017_AT WD repeat domain 7 rn
RG-U34C:RC_AI071577_AT WD repeat domain 7 rn
Protein Similarities
Method ID Description E-Value
blast AAK29933 Rab connectin related protein 2 [Caenorhabditis elegans] ref|NP_497573.2| RaB Connectin related family member (rbc-2) [Caenorhabditis elegans] 0.0
blast CAE65013 Hypothetical protein CBG09851 [Caenorhabditis briggsae] 0.0
blast XP_395749 PREDICTED: similar to GA14656-PA [Apis mellifera] 1.0E-109
blast XP_784047 PREDICTED: similar to WD repeat domain 7 protein isoform 2 [Strongylocentrotus purpuratus] 5.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0013
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.8E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.3
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0012
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.3
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0012
Sequence
Target Sequence 
>C. ELEGANS:176501_AT
gttgctcgatacaatgcagctgctcaacatcaaacaatccagcatacagtagtcagtcca
ttactgaaatctcgatcagaagttcttcgaataattgaagagttgtgcgagaaacgatac
aatgatataattgcaatgttactaccagttggagatattctcgttcattgcttggatatt
acaattctcaagcataaatcaatgtctgaggttttcccgccgattgtcaagtttaatatg
gtggcgtattgctcgacgagcaaacgtgttgcttttggtggaaagaatggaacttgtgtt
gtacatgagttgagagctactaaaactcattccctgccatctcataacggaccaatcgct
gccgtcgcgttcagcgaagatggaaaatatttggccacttatggagctgaagacggaaaa
attaactttttccagacaagccaatcgtttttgggaatgggacaagcccagctaaagctc
acaaaatcccaaccggctccaactgtatcagtaccaacaactcctagtggaacatcattc
cgtcctcgtttagtatggattaactctaaatcactgacacttatgcttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGCTCGATACAATGCAGCTGCTC 490 439 241 Antisense
GCAGCTGCTCAACATCAAACAATCC 421 313 256 Antisense
AATCAATGTCTGAGGTTTTCCCGCC 44 171 437 Antisense
ATGGTGGCGTATTGCTCGACGAGCA 170 51 478 Antisense
GAGCTACTAAAACTCATTCCCTGCC 475 387 554 Antisense
ATCTCATAACGGACCAATCGCTGCC 695 29 579 Antisense
TCGCTGCCGTCGCGTTCAGCGAAGA 200 601 596 Antisense
GCTAAAGCTCACAAAATCCCAACCG 101 303 711 Antisense
AACCGGCTCCAACTGTATCAGTACC 145 147 731 Antisense
TAGTGGAACATCATTCCGTCCTCGT 399 653 765 Antisense
TAAATCACTGACACTTATGCTTCCA 621 641 807 Antisense
Sequence Source Affymetrix Proprietary Database