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[176498_at]

Description Y53G8AL.3 /REP_DB=WormBase Gene ID /WP=CE26164 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 61      4grp_total > 43      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 14.1
(-3)
A 		55.1
(3)
A 		20.3
(-2.1)
A 		14.8
(-2.9)
A 		41
B 25
(-2.1)
A 		43.4
(3)
A 		21.8
(-3)
A 		25.4
(-2)
A 		22
C 24.5
(-3)
A 		31
(0.9)
A 		34.6
(3)
A 		33.1
(2.1)
A 		10
avr 21.2
(-3) 		43.2
(3) 		25.6
(-1.8) 		24.4
(-2.1) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 11
(-2.3)
A 		6.1
(-2.9)
A 		50.5
(3)
A 		6.3
(-2.9)
A 		10.2
(-2.4)
A 		5.7
(-3)
A 		45
B 35.2
(-0.2)
A 		8.9
(-2.7)
A 		68.2
(3)
A 		51.9
(1.4)
A 		30.4
(-0.6)
A 		5.5
(-3)
A 		63
C 28.5
(-1)
A 		6.6
(-2.7)
A 		39.4
(-0.2)
A 		80.5
(3)
A 		25.1
(-1.3)
A 		2.9
(-3)
A 		78
avr 24.9
(-0.5) 		7.2
(-2.7) 		52.7
(3) 		46.2
(2.2) 		21.9
(-0.9) 		4.7
(-3) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 5.1
(-1.2)
A 		12
(3)
A 		5.3
(-1.1)
A 		2.2
(-3)
A 		10
B 8
(0.1)
A 		10.7
(3)
A 		5.1
(-3)
A 		5.4
(-2.7)
A 		6
C 8.1
(-0.7)
A 		8.9
(0)
A 		12.1
(3)
A 		5.6
(-3)
A 		7
avr 7.1
(-0.3) 		10.5
(3) 		7.5
(0) 		4.4
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 2
(-2.6)
A 		2.2
(-2.5)
A 		11
(3)
A 		1.6
(-2.9)
A 		3.1
(-1.9)
A 		1.4
(-3)
A 		10
B 12.3
(1.2)
A 		3
(-2.6)
A 		16.6
(3)
A 		13.8
(1.8)
A 		12.3
(1.2)
A 		2
(-3)
A 		15
C 10.6
(0.8)
A 		2
(-2.6)
A 		10
(0.6)
A 		16.2
(3)
A 		8.1
(-0.2)
A 		1
(-3)
A 		15
avr 8.3
(0.7) 		2.4
(-2.5) 		12.5
(3) 		10.5
(1.9) 		7.8
(0.4) 		1.5
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176498_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6998
Sequence Type Exemplar sequence
Representative Public ID Y53G8AL.3 NCBI
Target Description Y53G8AL.3 /REP_DB=WormBase Gene ID /WP=CE26164 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065275(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065275 NCBI Caenorhabditis elegans Y53G8AL.3 (Y53G8AL.3) mRNA, complete cds. 11/11 None
Y53G8AL.3 ENSEMBL cdna:known chromosome:CEL140:III:3251450:3253132:-1 gene:Y53G8AL.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000028029 6/11 Cross Hyb Matching Probes None
SNAP00000028027 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3251449-3253132(-) 83.14 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AL.3
Entrez Gene 190232 Entrez gene
SwissProt Q95XY2 EMBL-EBI
WormBase CE26164 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84614 Hypothetical protein Y53G8AL.3 [Caenorhabditis elegans] ref|NP_497676.1| Y53G8AL.3 [Caenorhabditis elegans] 1.0E-130
blast CAB04435 Hypothetical protein F49C5.7 [Caenorhabditis elegans] ref|NP_496682.1| F49C5.7 [Caenorhabditis elegans] 8.0E-25
blast AAB66040 Hypothetical protein C35A11.3 [Caenorhabditis elegans] ref|NP_504428.1| C35A11.3 [Caenorhabditis elegans] 9.0E-23
Sequence
Target Sequence 
>C. ELEGANS:176498_AT
ggaatttgtttcgtcagctcatacgacgcaaaatgttctgctgattatttttgcagcgtc
aaaagagtgtggggtgaggggagagtgcgagagtgcgcgcgctgtctgcgtctcctctcc
ccctccactctctcaccactcttatcttgaaacctctatcgaaacctccaaatccgagca
gtgcggagcaaataggaagacaactagcatgagtttccaaaaatcttttgtcaccggcgc
ctgcctcacctacccctttatcggacaatcccttacctaccgtaaccaggaggatctagc
agctcaagattactcgaagattatgacttttaaggcaattacgtatataaccagagagca
gtattcaactcaaatagaggatttcttgaagttgagcccgagtttgaatgttttcgtggt
gcagtttgatgggacctcgagagccgtcctcctagacgctgccctacaaaactcgtgctc
caccgacacatctgactgcctggatttcaagtatgaagatgtgaactcaattttcttcac
tatttgctgggattcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAATTTGTTTCGTCAGCTCATACG 274 533 159 Antisense
GGGGAGAGTGCGAGAGTGCGCGCGC 611 497 236 Antisense
TCTCTCACCACTCTTATCTTGAAAC 445 611 287 Antisense
TGAAACCTCTATCGAAACCTCCAAA 214 571 306 Antisense
AACCTCCAAATCCGAGCAGTGCGGA 83 145 321 Antisense
TTACCTACCGTAACCAGGAGGATCT 63 619 431 Antisense
GATTTCTTGAAGTTGAGCCCGAGTT 454 423 538 Antisense
GAATGTTTTCGTGGTGCAGTTTGAT 351 331 564 Antisense
TTGATGGGACCTCGAGAGCCGTCCT 264 699 584 Antisense
ACACATCTGACTGCCTGGATTTCAA 450 111 644 Antisense
TTCTTCACTATTTGCTGGGATTCCA 627 689 691 Antisense
Sequence Source Affymetrix Proprietary Database