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[176496_s_at]

Description Y53G8AL.2 /REP_DB=WormBase Gene ID /WP=CE26163 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 14      6grp_total > 32      4grp_total > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3340
1 2 3 4 range
A 4406.4
(1.4)
P 		5214.8
(3)
P 		4944
(2.5)
P 		2229.4
(-3)
P 		2985
B 4955.4
(2)
P 		4854.4
(1.9)
P 		5450.6
(3)
P 		2332.7
(-3)
P 		3118
C 4805.2
(2.7)
P 		4476.7
(2)
P 		4958.9
(3)
P 		2110.6
(-3)
P 		2848
avr 4722.3
(2.2) 		4848.6
(2.4) 		5117.8
(3) 		2224.2
(-3) 		2894

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2588
1 2 3 4 5 6 range
A 4413.8
(2.3)
P 		4661.4
(3)
P 		4165.2
(1.7)
P 		3665.3
(0.3)
P 		2950.1
(-1.6)
P 		2430.6
(-3)
P 		2231
B 3731.6
(1.4)
P 		3931.8
(2.2)
P 		4154.4
(3)
P 		3724.5
(1.4)
P 		3589.4
(0.9)
P 		2539.3
(-3)
P 		1615
C 4155.6
(0.9)
P 		3554.2
(-0.5)
P 		5018.4
(3)
P 		4082.1
(0.8)
P 		3315.4
(-1.1)
P 		2503.6
(-3)
P 		2515
avr 4100.3
(1.9) 		4049.1
(1.8) 		4446
(3) 		3824
(1.1) 		3285
(-0.6) 		2491.2
(-3) 		1955
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1402
1 2 3 4 range
A 1604.4
(3)
P 		1132
(0.8)
P 		1292.5
(1.5)
P 		326.3
(-3)
P 		1278
B 1580.3
(3)
P 		1198.9
(0.9)
P 		1263
(1.2)
P 		497.1
(-3)
P 		1083
C 1590.6
(2.4)
P 		1287.1
(1.1)
P 		1728.3
(3)
P 		355.9
(-3)
P 		1372
avr 1591.8
(3) 		1206
(1.1) 		1427.9
(2.2) 		393.1
(-3) 		1199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1066
1 2 3 4 5 6 range
A 813.6
(-1.8)
P 		1657.8
(3)
P 		907.1
(-1.2)
P 		929.6
(-1.1)
P 		890.2
(-1.3)
P 		591.8
(-3)
P 		1066
B 1298.4
(1.1)
P 		1308.7
(1.2)
P 		1008.8
(-2.3)
P 		986.5
(-2.5)
P 		1458.3
(3)
P 		946.9
(-3)
P 		511
C 1540.3
(3)
P 		1084.5
(-0.8)
P 		1277.3
(0.8)
P 		820.9
(-3)
P 		1072.8
(-0.9)
P 		838.1
(-2.9)
P 		719
avr 1217.4
(1.6) 		1350.3
(3) 		1064.4
(-0.1) 		912.3
(-1.7) 		1140.4
(0.7) 		792.3
(-3) 		558
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176496_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6896
Sequence Type Exemplar sequence
Representative Public ID Y53G8AL.2 NCBI
Target Description Y53G8AL.2 /REP_DB=WormBase Gene ID /WP=CE26163 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065274(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065274 NCBI Caenorhabditis elegans Y53G8AL.2 (Y53G8AL.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000028030 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3241853:3248562:-1 11/11 None
SNAP00000028026 ENSEMBL cdna:SNAP chromosome:CEL140:III:3241868:3248562:-1 11/11 None
Y53G8AL.2.2 ENSEMBL cdna:known chromosome:CEL140:III:3241712:3248566:-1 gene:Y53G8AL.2 11/11 A 
Y53G8AL.2.1 ENSEMBL cdna:known chromosome:CEL140:III:3241712:3248564:-1 gene:Y53G8AL.2 11/11 A 
Y53G8AL.2.3 ENSEMBL cdna:known chromosome:CEL140:III:3241725:3248569:-1 gene:Y53G8AL.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3241852-3248562(-) 99.61 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AL.2
Entrez Gene 175429 Entrez gene
SwissProt Q9N3H3 EMBL-EBI
WormBase CE26163 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:12820_AT expressed protein at
ATH1-121501:265286_AT expressed protein at
CANINE:1604362_AT similar to NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial precursor (Complex I-39KD) (CI-39KD) cfa
CANINE_2:CFA.16743.1.S1_AT similar to NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial precursor (Complex I-39KD) (CI-39KD) cfa
CANINE_2:CFA.16743.1.S1_S_AT similar to NADH-ubiquinone oxidoreductase 39 kDa subunit, mitochondrial precursor (Complex I-39KD) (CI-39KD) cfa
DROSOPHILA_2:1633965_AT dm
DROSGENOME1:153309_AT dm
CHICKEN:GGAAFFX.13087.1.S1_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa gga
CHICKEN:GGA.1626.1.S1_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa gga
HG-U133A:208969_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HG-U133A_2:208969_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HG-U133_PLUS_2:208969_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HG-FOCUS:208969_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HUGENEFL:L04490_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
U133_X3P:G5326822_3P_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HG-U95D:73284_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HG-U95AV2:36205_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HG-U95E:72458_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
HU35KSUBB:RC_AA084343_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa hs
MG-U74AV2:96321_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 mm
MG-U74CV2:168982_I_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 mm
MOE430A:1416663_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 mm
MOUSE430A_2:1416663_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 mm
MOUSE430_2:1416663_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 mm
MU19KSUBA:TC19194_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, mRNA (cDNA clone IMAGE:3601317) mm
Protein Similarities
Method ID Description E-Value
blast AAF59528 Hypothetical protein Y53G8AL.2 [Caenorhabditis elegans] ref|NP_497675.1| Y53G8AL.2 [Caenorhabditis elegans] 0.0
blast CAE72965 Hypothetical protein CBG20301 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:176496_S_AT
gatttgggttggagacgttgccgccggaattcaaagtgctgtaaatgatccaacagcaaa
aggacatacttatgaatttgttggaccacactgctatcaattgtctgaattgattgattt
catgtacaaaaaagcgcattgtttgtctgattttggattcagatacaagcgtcacggaat
gccggatccatatttcatggctctcacaatggccaccgaactctatggtaaagtgttcaa
gtgtaaagttccactcaacagagaatggatggagtttgtggaagtacaatcggacatttt
gacgggcgaaagaactctcgccgacctcggagtccgccgattgactgaattcgaattggc
tggtggacaacaagcattttatcgatcatttaatcgatatttcgaagaacaatatggaga
acttccggctccatcacttccacttcgttcaccaccacttaccaaaaagaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTGGGTTGGAGACGTTGCCGCC 479 419 777 Antisense
CCGCCGGAATTCAAAGTGCTGTAAA 52 261 797 Antisense
GAATTTGTTGGACCACACTGCTATC 506 325 850 Antisense
GGACCACACTGCTATCAATTGTCTG 289 523 859 Antisense
AAGCGCATTGTTTGTCTGATTTTGG 502 149 908 Antisense
TGGATTCAGATACAAGCGTCACGGA 441 551 930 Antisense
AAGCGTCACGGAATGCCGGATCCAT 56 149 943 Antisense
GCCGGATCCATATTTCATGGCTCTC 16 285 957 Antisense
TTCATGGCTCTCACAATGGCCACCG 508 685 970 Antisense
AATGGCCACCGAACTCTATGGTAAA 12 163 984 Antisense
TCACCACCACTTACCAAAAAGAACA 612 623 1225 Antisense
Sequence Source Affymetrix Proprietary Database