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[176491_at]

Description Y74C10AR.B /REP_DB=WormBase Gene ID /WP=CE25603 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 27      4grp_total > 01      6grp_cgc > 15      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 466
1 2 3 4 range
A 251.7
(-2.5)
M 		213.3
(-3)
A 		288.6
(-1.9)
P 		637
(3)
P 		424
B 229.6
(-3)
A 		236.9
(-2.9)
A 		251
(-2.7)
A 		678.6
(3)
P 		449
C 212.6
(-3)
A 		215.7
(-2.9)
A 		275.2
(-1.8)
P 		534.2
(3)
P 		322
avr 231.3
(-2.9) 		222
(-3) 		271.6
(-2.2) 		616.6
(3) 		395

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 175.8
(3)
A 		153.7
(1.5)
A 		141.1
(0.6)
A 		90
(-3)
A 		108.9
(-1.7)
A 		146.5
(1)
A 		86
B 177.2
(3)
A 		119.4
(-1.6)
A 		109.9
(-2.4)
A 		111.5
(-2.3)
A 		102.5
(-3)
A 		137.9
(-0.2)
M 		75
C 152.4
(2.8)
A 		154.8
(3)
P 		103.5
(-1.7)
A 		108.8
(-1.2)
A 		89.4
(-3)
A 		120.3
(-0.2)
A 		65
avr 168.5
(3) 		142.6
(0.7) 		118.2
(-1.4) 		103.4
(-2.7) 		100.3
(-3) 		134.9
(0) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 91.6
(2.8)
M 		46.3
(-3)
A 		75.5
(0.7)
P 		93.2
(3)
P 		47
B 73.2
(-2)
A 		58.5
(-3)
A 		58.2
(-3)
A 		144.6
(3)
P 		86
C 70.4
(-1.5)
A 		62
(-3)
A 		95.9
(3)
P 		90.1
(2)
P 		34
avr 78.4
(-0.5) 		55.6
(-3) 		76.5
(-0.7) 		109.3
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 32.4
(-1.2)
A 		54.7
(3)
A 		30.7
(-1.5)
A 		22.8
(-3)
A 		32.9
(-1.1)
A 		35.7
(-0.6)
A 		32
B 61.7
(3)
A 		39.7
(-0.8)
A 		26.7
(-3)
A 		29.5
(-2.5)
A 		41.6
(-0.4)
A 		51.4
(1.2)
M 		35
C 56.5
(3)
A 		47.2
(1.4)
P 		26.3
(-2.2)
A 		21.9
(-3)
A 		28.9
(-1.8)
A 		40.3
(0.2)
A 		35
avr 50.2
(3) 		47.2
(2.3) 		27.9
(-2.2) 		24.7
(-3) 		34.5
(-0.7) 		42.5
(1.2) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176491_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18663
Sequence Type Exemplar sequence
Representative Public ID Y74C10AR.B NCBI
Target Description Y74C10AR.B /REP_DB=WormBase Gene ID /WP=CE25603 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058587(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058587 NCBI Caenorhabditis elegans Eukaryotic Initiation Factor family member (eif-3.I) (eif-3.I) mRNA, complete cds. 11/11 A 
Y74C10AR.1 ENSEMBL cdna:known chromosome:CEL140:I:2485870:2489500:1 gene:Y74C10AR.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2485869-2488599(+) 97.91 100.0
Public Domain and Genome References
Gene Title
Gene Symbol eif-3.I
Ensembl Y74C10AR.1
Entrez Gene 171809 Entrez gene
SwissProt Q965S8 EMBL-EBI
WormBase CE30325 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15177_S_AT eukaryotic translation initiation factor 3 subunit 2 / TGF-beta receptor interacting protein 1 / eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) at
ATH1-121501:266554_S_AT eukaryotic translation initiation factor 3 subunit 2 / TGF-beta receptor interacting protein 1 / eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) at
CANINE:1592124_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) cfa
CANINE_2:CFA.1036.1.S1_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) cfa
CANINE_2:CFA.8285.1.A1_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) cfa
CANINE_2:CFAAFFX.16721.1.S1_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) cfa
CANINE_2:CFAAFFX.16721.1.S1_S_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) cfa
DROSOPHILA_2:1630519_AT Trip1 dm
DROSGENOME1:154223_AT Trip1 dm
CHICKEN:GGA.7902.1.S1_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa gga
HG-U95AV2:1644_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HC-G110:1644_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HG-U133A:208756_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HG-U133_PLUS_2:208756_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HG-U133A_2:208756_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HG-FOCUS:208756_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HU35KSUBA:U36764_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HUGENEFL:U36764_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
U133_X3P:G1036804_3P_X_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
U133_X3P:208756_3P_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HG-U95AV2:32230_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
HG-U95C:58907_AT eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa hs
MU11KSUBA:AA036103_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MG-U74BV2:165275_R_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MG-U74CV2:171540_R_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MOE430A:1448264_A_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MOUSE430A_2:1448264_A_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MOUSE430_2:1448264_A_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MOE430A:1416233_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MOUSE430A_2:1416233_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MOUSE430_2:1416233_AT eukaryotic translation initiation factor 3, subunit 2 (beta) mm
MG-U74BV2:164585_F_AT Eukaryotic translation initiation factor 3, subunit 2 (beta), mRNA (cDNA clone MGC:25806 IMAGE:4037852) mm
MU19KSUBB:TC26516_AT Eukaryotic translation initiation factor 3, subunit 2 (beta), mRNA (cDNA clone MGC:25806 IMAGE:4037852) mm
RG-U34C:RC_AI176492_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) rn
RAE230A:1388328_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) rn
RAT230_2:1388328_AT similar to Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) rn
YEAST_2:1775293_AT Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation Sc
Protein Similarities
Method ID Description E-Value
blast AAK68601 Eukaryotic initiation factor protein 3.I [Caenorhabditis elegans] ref|NP_490988.2| Eukaryotic Initiation Factor family member (eif-3.I) [Caenorhabditis elegans] 0.0
blast CAE60390 Hypothetical protein CBG03991 [Caenorhabditis briggsae] 0.0
blast AAF52183 CG8882-PA [Drosophila melanogaster] gb|AAM49896.1| LD24026p [Drosophila melanogaster] ref|NP_523478.1| CG8882-PA [Drosophila melanogaster] gb|AAB53431.1| TRIP-1 homolog [Drosophila melanogaster] sp|O02195|IF32_DROME Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3i) (TRIP-1 homolog) 1.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.043
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.6E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0024
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.4E-9
Sequence
Target Sequence 
>C. ELEGANS:176491_AT
gacaaagaatctctcgacgttccaagtccgagatcttcgtgattcaagtcaaatggttga
gggtggagagtcattcttctactcacaattcgatgtgaacgccacgaccgcacttttcac
tcagatggatgacctcgtgacgatcggattcgagtctggactccttcagcagtacgattt
gagaaatccggatacaccaattcacactaacgagagtgttcatcgttacagcgtccaaga
tctccaactatcaccacgcggcgatttcctgatctccgcttcacgcgacaagacggccgc
cctgctcgacgtgaacgatctgaaaaagctgaaacaatacaaatccgagcgtcccgtcaa
ctcggcatgcatctcaccgaatcgcgatcacatttgccttggagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAAGAATCTCTCGACGTTCCAA 574 365 357 Antisense
GACGTTCCAAGTCCGAGATCTTCGT 639 377 372 Antisense
GGTGGAGAGTCATTCTTCTACTCAC 113 499 418 Antisense
CAGATGGATGACCTCGTGACGATCG 171 205 478 Antisense
GACGATCGGATTCGAGTCTGGACTC 387 379 495 Antisense
TGGACTCCTTCAGCAGTACGATTTG 511 545 513 Antisense
GAGAGTGTTCATCGTTACAGCGTCC 450 395 568 Antisense
CCCTGCTCGACGTGAACGATCTGAA 70 259 656 Antisense
AACAATACAAATCCGAGCGTCCCGT 275 133 689 Antisense
TCAACTCGGCATGCATCTCACCGAA 453 629 713 Antisense
TCGCGATCACATTTGCCTTGGAGGA 1 601 738 Antisense
Sequence Source Affymetrix Proprietary Database