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[176487_at]

Description Y54F10AM.C /REP_DB=WormBase Gene ID /WP=CE26171 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 03      4grp_total > 42      6grp_cgc > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 25.9
(-1)
A 		14.7
(-3)
A 		47.9
(3)
A 		35.8
(0.8)
A 		33
B 6.1
(-3)
A 		14.7
(-0.9)
A 		6
(-3)
A 		30.4
(3)
A 		24
C 30.3
(-0.4)
A 		61.4
(3)
A 		25.8
(-0.9)
A 		6.6
(-3)
A 		55
avr 20.8
(-3) 		30.3
(3) 		26.6
(0.7) 		24.3
(-0.8) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 5.2
(-3)
A 		30.6
(0.9)
A 		11.2
(-2.1)
A 		44.4
(3)
A 		19.1
(-0.9)
A 		15.9
(-1.4)
A 		39
B 6.2
(0.1)
A 		7.4
(3)
A 		4.9
(-3)
A 		6.2
(0.1)
A 		6.2
(0.1)
A 		6.8
(1.6)
A 		3
C 39
(3)
A 		15.7
(-1.6)
A 		9.1
(-2.9)
A 		9.1
(-2.9)
A 		10.3
(-2.6)
A 		8.4
(-3)
A 		31
avr 16.8
(1.4) 		17.9
(2) 		8.4
(-3) 		19.9
(3) 		11.9
(-1.2) 		10.4
(-2) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 9.4
(1)
A 		3.2
(-3)
A 		12.5
(3)
A 		5.2
(-1.7)
A 		9
B 2
(-2.3)
A 		3.6
(-0.4)
A 		1.4
(-3)
A 		6.5
(3)
A 		5
C 10
(0.2)
A 		17.7
(3)
A 		9
(-0.1)
A 		1.1
(-3)
A 		17
avr 7.1
(1.3) 		8.2
(3) 		7.6
(2.1) 		4.3
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 1
(-3)
A 		10.9
(2.8)
A 		2.4
(-2.2)
A 		11.3
(3)
A 		5.8
(-0.2)
A 		3.9
(-1.3)
A 		10
B 2.2
(1.6)
A 		2.5
(3)
A 		1.2
(-3)
A 		1.6
(-1.2)
A 		2.5
(3)
A 		2.5
(3)
A 		1
C 14.4
(3)
A 		4.8
(-1.6)
A 		2.3
(-2.8)
A 		1.8
(-3)
A 		3.3
(-2.3)
A 		2.8
(-2.5)
A 		13
avr 5.9
(2.7) 		6.1
(3) 		2
(-3) 		4.9
(1.2) 		3.9
(-0.2) 		3.1
(-1.4) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176487_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18905
Sequence Type Exemplar sequence
Representative Public ID Y54F10AM.C NCBI
Target Description Y54F10AM.C /REP_DB=WormBase Gene ID /WP=CE26171 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065172(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065172 NCBI Caenorhabditis elegans RaB Connectin related family member (rbc-2) (rbc-2) mRNA, complete cds. 11/11 None
SNAP00000034921 ENSEMBL cdna:SNAP chromosome:CEL140:III:2509485:2528616:-1 10/11 None
GENEFINDER00000034930 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2513255:2528616:-1 11/11 None
Y54F10AM.10 ENSEMBL cdna:known chromosome:CEL140:III:2512636:2528616:-1 gene:Y54F10AM.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2515332-2528616(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rbc-2
Ensembl Y54F10AM.10
Entrez Gene 190267 Entrez gene
SwissProt Q9BL05 EMBL-EBI
WormBase CE35950 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597274_AT similar to WD repeat domain 7 protein isoform 2 cfa
CANINE_2:CFAAFFX.1176.1.S1_S_AT similar to WD repeat domain 7 protein isoform 2 cfa
DROSOPHILA_2:1636006_AT dm
DROSGENOME1:143990_AT dm
CHICKEN:GGAAFFX.25716.1.S1_AT similar to WD repeat domain 7 protein isoform 2; TGF-beta resistance associated gene; rabconnectin-3 beta gga
HU35KSUBB:RC_F10381_S_AT WD repeat domain 7 hs
U133_X3P:HS.10881.0.S1_3P_AT WD repeat domain 7 hs
HG-U133A_2:212880_AT WD repeat domain 7 hs
HG-U133_PLUS_2:212880_AT WD repeat domain 7 hs
HG-U133A:212880_AT WD repeat domain 7 hs
HG-U95AV2:41430_AT WD repeat domain 7 hs
HG-U133_PLUS_2:241264_AT WD repeat domain 7 hs
HG-U133B:241264_AT WD repeat domain 7 hs
HG-U95C:56396_AT WD repeat domain 7 hs
HG-U95D:69252_I_AT WD repeat domain 7 hs
HG-U95D:69254_R_AT WD repeat domain 7 hs
HG-U95E:80292_AT WD repeat domain 7 hs
HG-U95D:84683_AT WD repeat domain 7 hs
U133_X3P:HS.164277.0.A1_3P_AT WD repeat domain 7 hs
HU35KSUBB:RC_AA218543_AT WD repeat domain 7 hs
HU35KSUBB:RC_T64012_AT WD repeat domain 7 hs
MG-U74BV2:113331_AT WD repeat domain 7 mm
MOE430B:1435028_AT WD repeat domain 7 mm
MOUSE430_2:1435028_AT WD repeat domain 7 mm
MOE430B:1441775_AT MKIAA0541 protein mm
MOUSE430_2:1441775_AT MKIAA0541 protein mm
MG-U74CV2:132607_AT MKIAA0541 protein mm
RAE230A:1369344_AT WD repeat domain 7 rn
RAT230_2:1369344_AT WD repeat domain 7 rn
RAE230A:1372833_AT WD repeat domain 7 rn
RAT230_2:1372833_AT WD repeat domain 7 rn
RAE230B:1397017_AT WD repeat domain 7 rn
RAT230_2:1397017_AT WD repeat domain 7 rn
RG-U34C:RC_AI071577_AT WD repeat domain 7 rn
Protein Similarities
Method ID Description E-Value
blast AAK29933 Rab connectin related protein 2 [Caenorhabditis elegans] ref|NP_497573.2| RaB Connectin related family member (rbc-2) [Caenorhabditis elegans] 0.0
blast CAE65013 Hypothetical protein CBG09851 [Caenorhabditis briggsae] 0.0
blast XP_395749 PREDICTED: similar to GA14656-PA [Apis mellifera] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0013
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.8E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.3
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0012
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.3
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0012
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.3
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.33
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0012
Sequence
Target Sequence 
>C. ELEGANS:176487_AT
gggcgcagagggacgatttccattcagtaattttgatttttttttgaaaacagtccgccg
cctcatcgaatcatggaccccattcctgccaccactcctcgatgattccctctcgatttt
cggctcaaaacttcaatcatccgttccaacagtccaaccaagtgctccggctcctccaat
ccctccacgcaccaaaaatgctccaccagacgtcgaacctgtccgtggatcggagccccc
gacagctgagggtggtgaagccggaattcaacaagttcgacgtaatcaagcaacttcgat
catcctgctcggtgtgattggctcacaatttggcgatgaactgaatcgcgccgatttgac
gagagccactgctgtgtcgttgctcgaattgctcgtcgcggctccttctaacctgattcc
agtgcattctccacttcgtcgggcggctattgatctattgggacggggatttgtacattg
ggagccgcatttagagatttccaaggtcgtcctggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGCGCAGAGGGACGATTTCCATTC 669 491 2721 Antisense
TCATCGAATCATGGACCCCATTCCT 528 621 2783 Antisense
GTCGAACCTGTCCGTGGATCGGAGC 50 473 2932 Antisense
GCCCCCGACAGCTGAGGGTGGTGAA 142 283 2955 Antisense
TCAAGCAACTTCGATCATCCTGCTC 558 631 3006 Antisense
GAATCGCGCCGATTTGACGAGAGCC 314 329 3063 Antisense
TGACGAGAGCCACTGCTGTGTCGTT 521 569 3077 Antisense
TAACCTGATTCCAGTGCATTCTCCA 652 637 3129 Antisense
TTCGTCGGGCGGCTATTGATCTATT 552 695 3155 Antisense
GGGGATTTGTACATTGGGAGCCGCA 666 495 3185 Antisense
TAGAGATTTCCAAGGTCGTCCTGGG 408 651 3212 Antisense
Sequence Source Affymetrix Proprietary Database