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[176475_s_at]

Description Y39G10AR.G /REP_DB=WormBase Gene ID /WP=CE21767 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 94      6grp_total > 69      4grp_total > 51      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 477
1 2 3 4 range
A 548.2
(0.8)
P 		668.9
(3)
P 		640.3
(2.5)
P 		342.4
(-3)
P 		327
B 566.1
(-0.5)
P 		800.6
(3)
P 		625.4
(0.4)
P 		395.5
(-3)
P 		405
C 513
(-0.7)
P 		807.1
(3)
P 		590.5
(0.3)
P 		329.9
(-3)
P 		477
avr 542.4
(-0.2) 		758.9
(3) 		618.7
(0.9) 		355.9
(-3) 		403

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 744
1 2 3 4 5 6 range
A 620.8
(0)
P 		363.2
(-2.2)
P 		266.7
(-3)
P 		349
(-2.3)
P 		603.3
(-0.1)
P 		966.8
(3)
P 		700
B 610.9
(-0.5)
P 		500.2
(-1.5)
P 		331.4
(-3)
P 		357
(-2.8)
P 		653.4
(-0.2)
P 		1010.7
(3)
P 		679
C 642.7
(0.6)
P 		353.8
(-3)
P 		382.2
(-2.6)
P 		427.9
(-2.1)
P 		775.2
(2.2)
P 		837
(3)
P 		483
avr 624.8
(-0.1) 		405.7
(-2.2) 		326.8
(-3) 		378
(-2.5) 		677.3
(0.4) 		938.2
(3) 		611
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 199.6
(3)
P 		145.2
(0.8)
P 		167.4
(1.7)
P 		50.1
(-3)
P 		150
B 180.5
(2.1)
P 		197.7
(3)
P 		144.9
(0.2)
P 		84.3
(-3)
P 		113
C 169.8
(0.9)
P 		232
(3)
P 		205.8
(2.1)
P 		55.6
(-3)
P 		176
avr 183.3
(2.6) 		191.6
(3) 		172.7
(2.1) 		63.3
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 319
1 2 3 4 5 6 range
A 114.4
(-1.1)
P 		129.2
(-0.6)
P 		58.1
(-3)
P 		88.5
(-2)
P 		182
(1.2)
P 		235.4
(3)
P 		177
B 212.6
(-0.3)
P 		166.5
(-1.3)
P 		80.5
(-3)
P 		94.6
(-2.7)
P 		265.5
(0.7)
P 		376.9
(3)
P 		296
C 238.2
(1.7)
P 		107.9
(-2.3)
P 		97.3
(-2.7)
P 		86.1
(-3)
P 		250.8
(2.1)
P 		280.2
(3)
P 		194
avr 188.4
(0) 		134.5
(-1.5) 		78.6
(-3) 		89.7
(-2.7) 		232.8
(1.2) 		297.5
(3) 		219
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176475_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18652
Sequence Type Exemplar sequence
Representative Public ID Y39G10AR.G NCBI
Target Description Y39G10AR.G /REP_DB=WormBase Gene ID /WP=CE21767 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058561(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058561 NCBI Caenorhabditis elegans yeast MCM (licensing factor) related family member (mcm-4) (mcm-4) mRNA, complete cds. 11/11 None
SNAP00000028457 ENSEMBL cdna:SNAP chromosome:CEL140:I:2318428:2321811:-1 11/11 None
GENEFINDER00000028491 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2318428:2321811:-1 11/11 None
Y39G10AR.14.2 ENSEMBL cdna:known chromosome:CEL140:I:2318295:2321825:-1 gene:Y39G10AR.14 11/11 None
Y39G10AR.14.1 ENSEMBL cdna:known chromosome:CEL140:I:2318295:2321823:-1 gene:Y39G10AR.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2318427-2321811(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mcm-4
Ensembl Y39G10AR.14
Entrez Gene 171793 Entrez gene
SwissProt Q95XQ8 EMBL-EBI
WormBase CE21767 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16800_AT DNA replication licensing factor, putative at
ATH1-121501:263612_AT DNA replication licensing factor, putative at
CANINE_2:CFA.18077.1.S1_S_AT similar to minichromosome maintenance protein 4 cfa
CANINE_2:CFA.19941.1.S1_S_AT similar to minichromosome maintenance protein 4 cfa
CANINE_2:CFAAFFX.10864.1.S1_AT similar to minichromosome maintenance protein 4 cfa
DROSOPHILA_2:1629737_AT disc proliferation abnormal dm
DROSGENOME1:143860_AT disc proliferation abnormal dm
CHICKEN:GGA.7164.1.S1_AT similar to DNA replication initiator protein gga
HG-U95AV2:981_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HC-G110:981_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBA:HUM_ALU_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBB:HUM_ALU_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBC:HUM_ALU_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBD:HUM_ALU_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HUGENEFL:HUM_ALU_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HC-G110:HUM_ALU_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBA:X74794_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HUGENEFL:X74794_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBC:RC_N27515_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
U133_X3P:HS.296277.0.S1_3P_S_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
U133_X3P:HS.154443.0.S2_3P_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
U133_X3P:HS.154443.0.S1_3P_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A_2:212141_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133_PLUS_2:212141_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A:212141_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A_2:212142_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133_PLUS_2:212142_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A:212142_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133_PLUS_2:242479_S_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133B:242479_S_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U95C:52597_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133_PLUS_2:222036_S_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A_2:222036_S_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A:222036_S_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBC:RC_AA148885_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
U133_X3P:HS.154443.1.S1_3P_X_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
U133_X3P:HS.154443.1.S1_3P_A_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A_2:222037_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133_PLUS_2:222037_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U133A:222037_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U95C:63855_R_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U95B:42875_R_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HG-U95E:69711_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
HU35KSUBB:RC_R07012_AT MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) hs
MU11KSUBA:D26089_S_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MG-U74AV2:93041_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MOE430A:1436708_X_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MOUSE430A_2:1436708_X_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MOUSE430_2:1436708_X_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MOE430A:1416214_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MOUSE430A_2:1416214_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MOUSE430_2:1416214_AT minichromosome maintenance deficient 4 homolog (S. cerevisiae) mm
MU19KSUBB:TC29218_S_AT Minichromosome maintenance deficient 4 homolog (S. cerevisiae), mRNA (cDNA clone MGC:6023 IMAGE:3584640) mm
RAE230A:1373557_AT mini chromosome maintenance deficient 4 homolog (S. cerevisiae) rn
RAT230_2:1373557_AT mini chromosome maintenance deficient 4 homolog (S. cerevisiae) rn
YEAST_2:1776004_AT Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt the DNA prior to replication; accumulates in the nucleus in G1; homolog of S. pombe Cdc21p Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6270 DNA replication initiation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8094 DNA-dependent ATPase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_490962 yeast MCM (licensing factor) related family member (mcm-4) [Caenorhabditis elegans] gb|AAK39605.1| Yeast mcm (licensing factor) related protein 4 [Caenorhabditis elegans] 0.0
blast CAE60303 Hypothetical protein CBG03890 [Caenorhabditis briggsae] 0.0
blast AAH65958 Mcm4 protein [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001208 EMBL-EBI MCM 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176475_S_AT
agactcggaaatcatctcgtctcgctctactttgagaatgacggtaatcaggagaagacg
gagcatgtggacatgaacttgctcagagattatatcgcctacgcaaaagcgaatatccat
ccgaaactcagcgaagaggcatcacaatttatcattgaaaagtatctattcatgagaaaa
gctggcgcgcagcacggacaaatcaccgcctacccgagacagttggagtcgttgattcgt
ctttcagaggctcacgccaagattcgtctttcacaagaggtgtctgtggatgatgtggag
aaggctttcacattgtggcgtgaggctctcaaacaatcggccgtcgatccatccacggga
cgtgtcgacgtggcaattctggcatctggaatgagtgcttctggaagaaaggcggtcgag
gcgatgtgtgaggccgttctgaagcagctcaagactgccaagggatttgtgacctctaag
gctctgttccatactctcaaatctgctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACTCGGAAATCATCTCGTCTCGC 183 71 1870 Antisense
AGCGAATATCCATCCGAAACTCAGC 59 83 1977 Antisense
CCGCCTACCCGAGACAGTTGGAGTC 369 261 2075 Antisense
GTTGATTCGTCTTTCAGAGGCTCAC 102 439 2100 Antisense
AGAGGCTCACGCCAAGATTCGTCTT 181 69 2115 Antisense
TGAGGCTCTCAAACAATCGGCCGTC 464 565 2190 Antisense
TCGATCCATCCACGGGACGTGTCGA 520 603 2213 Antisense
GTGTCGACGTGGCAATTCTGGCATC 559 485 2231 Antisense
GTGTGAGGCCGTTCTGAAGCAGCTC 552 487 2295 Antisense
TTGTGACCTCTAAGGCTCTGTTCCA 266 707 2336 Antisense
TGTTCCATACTCTCAAATCTGCTGA 292 563 2354 Antisense
Sequence Source Affymetrix Proprietary Database