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[176474_at]

Description Y54F10AM.H /REP_DB=WormBase Gene ID /WP=CE26176 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      6grp_highest_nonorm > 53      6grp_total > 70      4grp_total > 49      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 637
1 2 3 4 range
A 5
(-3)
A 		537.7
(3)
P 		522.3
(2.8)
P 		48.6
(-2.5)
A 		533
B 17
(-3)
A 		642.2
(3)
P 		543.3
(2.1)
P 		68.8
(-2.5)
A 		625
C 47.6
(-3)
A 		558.8
(3)
P 		549.8
(2.9)
P 		69.7
(-2.7)
A 		511
avr 23.2
(-3) 		579.6
(3) 		538.5
(2.6) 		62.4
(-2.6) 		556

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1371
1 2 3 4 5 6 range
A 521.4
(-2)
P 		440.9
(-2.3)
P 		1051.9
(0.5)
P 		1583.8
(3)
P 		665.6
(-1.3)
P 		296.6
(-3)
P 		1287
B 285.7
(-2.7)
P 		500.3
(-1.6)
P 		966
(0.8)
P 		1388.4
(3)
P 		861.2
(0.3)
P 		236.1
(-3)
P 		1152
C 388.5
(-2.2)
P 		422.9
(-2)
P 		859.4
(-0)
P 		1513.7
(3)
P 		735.6
(-0.6)
P 		213.1
(-3)
P 		1301
avr 398.5
(-2.3) 		454.7
(-2) 		959.1
(0.4) 		1495.3
(3) 		754.1
(-0.6) 		248.6
(-3) 		1247
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 1.8
(-3)
A 		116.7
(2.1)
P 		136.5
(3)
P 		7.1
(-2.8)
A 		135
B 5.4
(-3)
A 		158.6
(3)
P 		125.9
(1.7)
P 		14.7
(-2.6)
A 		153
C 15.7
(-2.9)
A 		160.7
(2)
P 		191.6
(3)
P 		11.8
(-3)
A 		180
avr 7.6
(-3) 		145.3
(2.7) 		151.3
(3) 		11.2
(-2.8) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 330
1 2 3 4 5 6 range
A 96.1
(-2.6)
P 		156.8
(-1.5)
P 		229.1
(-0.1)
P 		401.7
(3)
P 		200.8
(-0.7)
P 		72.2
(-3)
P 		330
B 99.4
(-2.8)
P 		166.5
(-1.3)
P 		234.6
(0.1)
P 		367.7
(3)
P 		349.9
(2.6)
P 		88.1
(-3)
P 		280
C 144
(-1.1)
P 		129
(-1.5)
P 		218.7
(0.8)
P 		304.4
(3)
P 		238
(1.3)
P 		71.3
(-3)
P 		233
avr 113.2
(-2.2) 		150.8
(-1.4) 		227.5
(0.2) 		357.9
(3) 		262.9
(1) 		77.2
(-3) 		281
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176474_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18957
Sequence Type Exemplar sequence
Representative Public ID Y54F10AM.H NCBI
Target Description Y54F10AM.H /REP_DB=WormBase Gene ID /WP=CE26176 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065170(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065170 NCBI Caenorhabditis elegans Y54F10AM.6 (Y54F10AM.6) mRNA, complete cds. 11/11 None
GENEFINDER00000034938 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2508612:2512196:1 11/11 None
SNAP00000034920 ENSEMBL cdna:SNAP chromosome:CEL140:III:2509080:2531689:1 11/11 None
Y54F10AM.6 ENSEMBL cdna:known chromosome:CEL140:III:2509080:2511630:1 gene:Y54F10AM.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2509079-2511630(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54F10AM.6
Entrez Gene 175370 Entrez gene
SwissProt Q9BL06 EMBL-EBI
WormBase CE26176 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29935 Hypothetical protein Y54F10AM.6 [Caenorhabditis elegans] ref|NP_497571.1| Y54F10AM.6 [Caenorhabditis elegans] 1.0E-144
blast AAK29935 Hypothetical protein Y54F10AM.6 [Caenorhabditis elegans] ref|NP_497571.1| Y54F10AM.6 [Caenorhabditis elegans] 1.0E-142
blast AAK29935 Hypothetical protein Y54F10AM.6 [Caenorhabditis elegans] ref|NP_497571.1| Y54F10AM.6 [Caenorhabditis elegans] 1.0E-134
blast CAE65014 Hypothetical protein CBG09852 [Caenorhabditis briggsae] 1.0E-119
blast CAE65014 Hypothetical protein CBG09852 [Caenorhabditis briggsae] 1.0E-118
blast CAE65014 Hypothetical protein CBG09852 [Caenorhabditis briggsae] 1.0E-117
blast AAK29936 Hypothetical protein Y54F10AM.5 [Caenorhabditis elegans] ref|NP_497574.2| Y54F10AM.5 [Caenorhabditis elegans] 8.0E-94
blast CAE69224 Hypothetical protein CBG15264 [Caenorhabditis briggsae] 5.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR010625 EMBL-EBI CHCH 4.1E-8
Sequence
Target Sequence 
>C. ELEGANS:176474_AT
aaccatacctcgaacaaatctcctcaatttgccaacaccattggttctacagttttataa
aacgatatgaaaaatcattgaacagtttctactcatatatgggaagtgtactgaaatcca
aaaaaccatgtggactctgctcttacaagcaatcgtgtggattcggtggagctgtaaagt
gtaatcagagcccgttgtcagtagaaggaacccggcctctgattccattttatgtcgctg
aacgagtttgcagtgagaaagatcttggaaagagtcaagtggattcttgtgttgttgatt
atgatgcattgatggcaaacgggagggaatgccaactatggccatctacaaaagtggatt
tatcagatattgagccagcattccgtgagcatgtccataatcttgcgtggtacacgtgtc
ttccgcagaacagaaaactccgaaaaggtcgcggaaagaatatcaaattccgcaccgaaa
aagtatgccgctgctgttgttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCATACCTCGAACAAATCTCCTC 591 145 158 Antisense
CAAATCTCCTCAATTTGCCAACACC 478 193 172 Antisense
AAAAACCATGTGGACTCTGCTCTTA 612 129 278 Antisense
AGTGTAATCAGAGCCCGTTGTCAGT 274 57 335 Antisense
AGTAGAAGGAACCCGGCCTCTGATT 628 53 357 Antisense
GGCCTCTGATTCCATTTTATGTCGC 328 543 371 Antisense
GGGAATGCCAACTATGGCCATCTAC 263 493 482 Antisense
TATTGAGCCAGCATTCCGTGAGCAT 255 665 525 Antisense
GAGCATGTCCATAATCTTGCGTGGT 144 389 544 Antisense
TTGCGTGGTACACGTGTCTTCCGCA 70 703 560 Antisense
AAAAAGTATGCCGCTGCTGTTGTTT 560 129 635 Antisense
Sequence Source Affymetrix Proprietary Database