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[176470_at]

Description Y54F10AM.I /REP_DB=WormBase Gene ID /WP=CE26177 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 17      4grp_high_8_8 > 60      6grp_highest_nonorm > 34      6grp_total > 51      4grp_total > 59      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1968
1 2 3 4 range
A 2397
(0.7)
P 		2458.1
(0.9)
P 		3161.7
(3)
P 		1194
(-3)
P 		1968
B 2656.9
(1.6)
P 		2477.4
(1)
P 		3047.4
(3)
P 		1338.8
(-3)
P 		1709
C 2202.5
(0.4)
P 		2773.8
(2.7)
P 		2851.3
(3)
P 		1376.3
(-3)
P 		1475
avr 2418.8
(0.9) 		2569.8
(1.4) 		3020.1
(3) 		1303
(-3) 		1717

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2822
1 2 3 4 5 6 range
A 2338.3
(-1.4)
P 		3151.2
(0.8)
P 		2763.8
(-0.3)
P 		3954.5
(3)
P 		1762.5
(-3)
P 		2477.3
(-1)
P 		2192
B 1700.6
(-1.6)
P 		2086.6
(-0.1)
P 		2886.6
(3)
P 		2215.5
(0.4)
P 		1775
(-1.3)
P 		1350.2
(-3)
P 		1536
C 3088.4
(0.5)
P 		2323.9
(-1.3)
P 		4172.6
(3)
P 		2505.2
(-0.8)
P 		2553.2
(-0.7)
P 		1567.9
(-3)
P 		2605
avr 2375.8
(-0.7) 		2520.6
(-0.1) 		3274.3
(3) 		2891.7
(1.4) 		2030.2
(-2.1) 		1798.5
(-3) 		1476
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 819
1 2 3 4 range
A 872.7
(3)
P 		533.6
(0.1)
P 		826.5
(2.6)
P 		174.8
(-3)
P 		698
B 847.3
(3)
P 		611.9
(0.5)
P 		706.1
(1.5)
P 		285.3
(-3)
P 		562
C 729.1
(0.9)
P 		797.5
(1.5)
P 		993.8
(3)
P 		232.1
(-3)
P 		762
avr 816.4
(2.7) 		647.7
(1.1) 		842.1
(3) 		230.7
(-3) 		611

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 714
1 2 3 4 5 6 range
A 431.1
(-3)
P 		1120.7
(3)
P 		601.9
(-1.5)
P 		1003
(2)
P 		531.8
(-2.1)
P 		603.1
(-1.5)
P 		690
B 591.7
(-0.6)
P 		694.6
(2.3)
P 		700.9
(2.4)
P 		586.8
(-0.7)
P 		721.2
(3)
P 		503.5
(-3)
P 		218
C 1144.7
(3)
P 		709.1
(-1.1)
P 		1062
(2.2)
P 		503.8
(-3)
P 		826.2
(0)
P 		524.9
(-2.8)
P 		641
avr 722.5
(0.6) 		841.5
(3) 		788.3
(1.9) 		697.9
(0.1) 		693.1
(0) 		543.8
(-3) 		298
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176470_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18940
Sequence Type Exemplar sequence
Representative Public ID Y54F10AM.I NCBI
Target Description Y54F10AM.I /REP_DB=WormBase Gene ID /WP=CE26177 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065173(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065173 NCBI Caenorhabditis elegans Y54F10AM.5 (Y54F10AM.5) mRNA, complete cds. 10/11 None
SNAP00000034920 ENSEMBL cdna:SNAP chromosome:CEL140:III:2509080:2531689:1 10/11 None
GENEFINDER00000034931 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2529064:2530341:1 10/11 None
Y54F10AM.5.1 ENSEMBL cdna:known chromosome:CEL140:III:2529045:2530491:1 gene:Y54F10AM.5 10/11 None
Y54F10AM.5.2 ENSEMBL cdna:known chromosome:CEL140:III:2529051:2530412:1 gene:Y54F10AM.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2528782-2530341(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54F10AM.5
Entrez Gene 175371 Entrez gene
SwissProt Q9BL03 EMBL-EBI
WormBase CE34127 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1583152_AT similar to NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa cfa
CANINE_2:CFA.714.1.A1_S_AT similar to NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa cfa
DROSOPHILA_2:1634417_S_AT dm
DROSGENOME1:147881_AT dm
HG-U133_PLUS_2:218160_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
HG-U133A:218160_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
HG-FOCUS:218160_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
HG-U133A_2:218160_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
HU35KSUBA:W78211_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
HU35KSUBB:RC_W94366_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
U133_X3P:G7657368_3P_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
HG-U95B:43795_S_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa hs
MG-U74AV2:93531_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 mm
MOE430A:1423692_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 mm
MOUSE430A_2:1423692_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 mm
MOUSE430_2:1423692_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 mm
MU19KSUBC:TC40863_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (Ndufa8), mRNA mm
RT-U34:RC_AI232012_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (predicted) rn
RG-U34A:RC_AI232012_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (predicted) rn
RAE230A:1371355_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (predicted) rn
RAT230_2:1371355_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (predicted) rn
RAE230B:1392167_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (predicted) rn
RAT230_2:1392167_AT NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAK29935 Hypothetical protein Y54F10AM.6 [Caenorhabditis elegans] ref|NP_497571.1| Y54F10AM.6 [Caenorhabditis elegans] 1.0E-142
blast CAE65014 Hypothetical protein CBG09852 [Caenorhabditis briggsae] 1.0E-118
blast AAK29936 Hypothetical protein Y54F10AM.5 [Caenorhabditis elegans] ref|NP_497574.2| Y54F10AM.5 [Caenorhabditis elegans] 1.0E-103
blast CAE65012 Hypothetical protein CBG09850 [Caenorhabditis briggsae] 7.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR010625 EMBL-EBI CHCH 4.1E-8
Pfam IPR010625 EMBL-EBI CHCH 4.1E-8
Sequence
Target Sequence 
>C. ELEGANS:176470_AT
ggcatgcgcctttaaacttggtacattttccgatgagtataaccaaggatacgcattttc
caagtgacgaggagctcactgttcctcaggaaatcactttatcaactccatggctcaaat
ccatcgctccctatatggcaaaacattgcgaaaaggaggcaaatgagttcatgcttcgac
gaaaggaagccgaggatccacgtgccgttttgaaggagggagccgcgctgaccgcgtgtg
gagtcaattttcttcaatctttgaagcgttcctgccttccacagactcagaagctcgccg
aatgtgttgatcagagcagtgccaagctttacatgtcaaagtgccatgacgaccagaagg
agctcgatgcctgcgtggaagctaatttgaacttgacgcgtccaaagcttggatacttca
gcaaacttcatgtctatgactcggccaccgcggctccagaggtgaagctccgtgactaca
aggcagaagcagctaaagtgctcaatgagcttccagccgactatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCATGCGCCTTTAAACTTGGTACA 454 535 47 Antisense
AATCACTTTATCAACTCCATGGCTC 372 171 138 Antisense
AAAGGAAGCCGAGGATCCACGTGCC 409 121 228 Antisense
ACCGCGTGTGGAGTCAATTTTCTTC 19 91 277 Antisense
TTCAATCTTTGAAGCGTTCCTGCCT 428 685 299 Antisense
GCCTTCCACAGACTCAGAAGCTCGC 367 281 320 Antisense
GAAGCTCGCCGAATGTGTTGATCAG 414 343 336 Antisense
GAACTTGACGCGTCCAAAGCTTGGA 160 349 435 Antisense
CCACCGCGGCTCCAGAGGTGAAGCT 636 273 491 Antisense
GGTGAAGCTCCGTGACTACAAGGCA 176 497 507 Antisense
TCAATGAGCTTCCAGCCGACTATCA 267 633 548 Antisense
Sequence Source Affymetrix Proprietary Database