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[176462_at]

Description Y39G10AR.U /REP_DB=WormBase Gene ID /WP=CE23001 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 01      4grp_total > 44      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 8.6
(0.3)
A 		5.5
(-3)
A 		6
(-2.5)
A 		11.1
(3)
A 		6
B 19
(-1.6)
A 		59.7
(3)
A 		6.9
(-3)
A 		13.9
(-2.2)
A 		53
C 39.4
(3)
A 		6.9
(-2.6)
A 		4.7
(-3)
A 		8.7
(-2.3)
A 		35
avr 22.3
(2.4) 		24
(3) 		5.9
(-3) 		11.2
(-1.2) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 25.9
(1.7)
A 		26.8
(1.9)
A 		5.2
(-3)
A 		6.2
(-2.8)
A 		31.8
(3)
A 		14.1
(-1)
A 		27
B 9.7
(-2.3)
A 		10.1
(-2.2)
A 		33.3
(3)
A 		11.2
(-2)
A 		9.5
(-2.3)
A 		6.6
(-3)
A 		27
C 11
(-1.5)
A 		26.5
(3)
A 		6.8
(-2.7)
A 		8.6
(-2.2)
A 		5.9
(-3)
A 		15.2
(-0.3)
A 		21
avr 15.5
(0.3) 		21.1
(3) 		15.1
(0.1) 		8.7
(-3) 		15.7
(0.4) 		12
(-1.4) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 3.1
(3)
A 		1.2
(-3)
A 		1.6
(-1.7)
A 		1.6
(-1.7)
A 		2
B 6.1
(-0.9)
A 		14.7
(3)
A 		1.6
(-3)
A 		3
(-2.4)
A 		13
C 13
(3)
A 		2
(-2.7)
A 		1.6
(-2.9)
A 		1.5
(-3)
A 		12
avr 7.4
(3) 		6
(1.6) 		1.6
(-3) 		2
(-2.6) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 4.8
(-0.4)
A 		9.5
(2.9)
A 		1.1
(-3)
A 		1.6
(-2.6)
A 		9.6
(3)
A 		3.4
(-1.4)
A 		9
B 3.4
(-2)
A 		3.4
(-2)
A 		8.1
(3)
A 		3
(-2.5)
A 		3.9
(-1.5)
A 		2.5
(-3)
A 		6
C 4.1
(-0.8)
A 		8.1
(3)
A 		1.7
(-3)
A 		1.7
(-3)
A 		1.9
(-2.8)
A 		5.1
(0.2)
A 		6
avr 4.1
(-0.6) 		7
(3) 		3.6
(-1.2) 		2.1
(-3) 		5.1
(0.7) 		3.7
(-1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176462_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18683
Sequence Type Exemplar sequence
Representative Public ID Y39G10AR.U NCBI
Target Description Y39G10AR.U /REP_DB=WormBase Gene ID /WP=CE23001 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058567(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058567 NCBI Caenorhabditis elegans Y39G10AR.3 (Y39G10AR.3) mRNA, complete cds. 11/11 None
GENEFINDER00000028472 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2358421:2369751:1 11/11 None
Y39G10AR.3 ENSEMBL cdna:known chromosome:CEL140:I:2357884:2372965:1 gene:Y39G10AR.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028467 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2357883-2365249(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y39G10AR.3
Entrez Gene 189769 Entrez gene
SwissProt Q6AW07 EMBL-EBI
WormBase CE37063 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.28700.1.S1_S_AT similar to NIMA-related kinase 8 cfa
DROSOPHILA_2:1635964_AT nimA-like kinase dm
DROSGENOME1:150526_AT nimA-like kinase dm
CHICKEN:GGAAFFX.26076.1.S1_AT similar to NIMA-related kinase 8; NIMA-related kinase 12a gga
HU35KSUBA:HUM_ALU_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HU35KSUBB:HUM_ALU_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HU35KSUBC:HUM_ALU_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HU35KSUBD:HUM_ALU_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HUGENEFL:HUM_ALU_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HC-G110:HUM_ALU_AT NIMA (never in mitosis gene a)- related kinase 8 hs
U133_X3P:HS2.293894.1.S1_3P_X_AT NIMA (never in mitosis gene a)- related kinase 8 hs
U133_X3P:HS2.293894.1.S1_3P_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HG-U133_PLUS_2:1557170_AT NIMA (never in mitosis gene a)- related kinase 8 hs
HG-U133_PLUS_2:1557172_X_AT NIMA (never in mitosis gene a)- related kinase 8 hs
MOUSE430_2:1458157_AT NIMA (never in mitosis gene a)-related expressed kinase 8 mm
MOE430B:1458157_AT NIMA (never in mitosis gene a)-related expressed kinase 8 mm
MOUSE430_2:1450337_A_AT NIMA (never in mitosis gene a)-related expressed kinase 8 mm
MOE430A:1450337_A_AT NIMA (never in mitosis gene a)-related expressed kinase 8 mm
MOUSE430A_2:1450337_A_AT NIMA (never in mitosis gene a)-related expressed kinase 8 mm
MU19KSUBA:TC16376_AT NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_490968 Y39G10AR.3 [Caenorhabditis elegans] gb|AAT81178.1| Hypothetical protein Y39G10AR.3 [Caenorhabditis elegans] 0.0
blast CAE60376 Hypothetical protein CBG03977 [Caenorhabditis briggsae] 0.0
blast CAE60375 Hypothetical protein CBG03976 [Caenorhabditis briggsae] 1.0E-134
blast NP_620776 NIMA (never in mitosis gene a)-related kinase 8 [Danio rerio] sp|Q90XC2|NEK8_BRARE Serine/threonine-protein kinase Nek8 (NimA-related protein kinase 8) gb|AAL09676.1| NIMA-related kinase 8 [Danio rerio] 6.0E-80
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-52
hanks 2.3.2 CaMK Group; CaMK Other; PfCPK 8.0E-53
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 3.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000408 EMBL-EBI Regulator of chromosome condensation, RCC1 9.2E-5
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-5
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 6.5E-82
Sequence
Target Sequence 
>C. ELEGANS:176462_AT
aatcttcccgcgttggtcaatagtattatgacgtgtgcctacacaccagtaaaaggacct
tactcggctgaaatgaaaatggtgatacgggaattgctacagttggatccgcaaaagagg
ccctccgctccgcaggcgctaaagatgctgcgaccttcggaaaaccgtcaccgacacacc
tccggatcaatgagatcttcggtttcgttcacaactctctacgatttgcatgtcccgaca
atcaccctgtcctcaattccagaccttcccagccggatatcgattaagcaggtcgctctg
agcaaaactcatacaatggtgctcacaaatgataatgaacttttcggatttggtgataac
tcttgtggacaactgggattcgggaagttttcgaaaatggaaaacgggcggttggtggag
agtttgaagggcagagatatacaatcagttggggttgggaattattttagtgtaatctgc
tgtgatcgggggaccttattgacatttggtactgcaaagaacctcggtttggggaaagat
gcaaaagattcagcgaagccgatgctcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCTTCCCGCGTTGGTCAATAGTA 99 169 1135 Antisense
AAGGACCTTACTCGGCTGAAATGAA 325 161 1187 Antisense
AGTTGGATCCGCAAAAGAGGCCCTC 420 61 1235 Antisense
CGCTCCGCAGGCGCTAAAGATGCTG 483 255 1260 Antisense
GCTGCGACCTTCGGAAAACCGTCAC 380 295 1281 Antisense
GACACACCTCCGGATCAATGAGATC 263 367 1307 Antisense
CGTTCACAACTCTCTACGATTTGCA 536 247 1340 Antisense
CGATTTGCATGTCCCGACAATCACC 587 247 1356 Antisense
ACCTTCCCAGCCGGATATCGATTAA 265 85 1397 Antisense
TAATCTGCTGTGATCGGGGGACCTT 593 633 1607 Antisense
AAGATTCAGCGAAGCCGATGCTCCT 1 157 1679 Antisense
Sequence Source Affymetrix Proprietary Database