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[176446_s_at]

Description Y39G10AR.P /REP_DB=WormBase Gene ID /WP=CE21763 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 20      6grp_total > 06      4grp_total > 33      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 908
1 2 3 4 range
A 1591.1
(-3)
P 		2401
(2.6)
P 		2115.5
(0.6)
P 		2463.3
(3)
P 		872
B 1555.4
(-3)
P 		1983.9
(0.3)
P 		2327.6
(3)
P 		2311.2
(2.9)
P 		772
C 1610.6
(-3)
P 		2001.1
(0.8)
P 		1767.8
(-1.5)
P 		2220.8
(3)
P 		610
avr 1585.7
(-3) 		2128.7
(1.4) 		2070.3
(0.9) 		2331.8
(3) 		746

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1049
1 2 3 4 5 6 range
A 1317.6
(3)
P 		1291.4
(2.7)
P 		804.4
(-3)
P 		914.6
(-1.7)
P 		952.5
(-1.3)
P 		1091.7
(0.4)
P 		513
B 1098
(2.2)
P 		1072.1
(1.4)
P 		1125.5
(3)
P 		932.2
(-2.8)
P 		926.9
(-3)
P 		936.8
(-2.7)
P 		199
C 1768.2
(3)
P 		991.4
(-1.4)
P 		1245.4
(0)
P 		734.8
(-2.9)
P 		1109.1
(-0.8)
P 		719.3
(-3)
P 		1049
avr 1394.6
(3) 		1118.3
(-0.1) 		1058.4
(-0.8) 		860.5
(-3) 		996.2
(-1.5) 		915.9
(-2.4) 		534
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 579.3
(3)
P 		521.2
(1.4)
P 		553
(2.3)
P 		360.6
(-3)
P 		219
B 496
(-2.3)
P 		490
(-3)
P 		539.4
(3)
P 		492.5
(-2.7)
P 		49
C 533.1
(0.9)
P 		575.3
(2)
P 		616.1
(3)
P 		374.5
(-3)
P 		242
avr 536.1
(1.7) 		528.8
(1.5) 		569.5
(3) 		409.2
(-3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 508
1 2 3 4 5 6 range
A 242.9
(-1.6)
P 		459.3
(3)
P 		175.2
(-3)
P 		232
(-1.8)
P 		287.4
(-0.6)
P 		265.8
(-1.1)
P 		284
B 382
(3)
P 		356.9
(1.9)
P 		273.3
(-1.8)
P 		246.9
(-3)
P 		376.6
(2.8)
P 		349.3
(1.5)
P 		135
C 655.4
(3)
P 		302.5
(-1.2)
P 		317
(-1)
P 		147.8
(-3)
P 		358.9
(-0.5)
P 		240.8
(-1.9)
P 		508
avr 426.8
(3) 		372.9
(1.5) 		255.2
(-1.7) 		208.9
(-3) 		341
(0.6) 		285.3
(-0.9) 		218
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176446_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18682
Sequence Type Exemplar sequence
Representative Public ID Y39G10AR.P NCBI
Target Description Y39G10AR.P /REP_DB=WormBase Gene ID /WP=CE21763 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058559(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058559 NCBI Caenorhabditis elegans Y39G10AR.8 (Y39G10AR.8) mRNA, complete cds. 11/11 None
GENEFINDER00000028492 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2312511:2315761:1 11/11 None
SNAP00000028455 ENSEMBL cdna:SNAP chromosome:CEL140:I:2313312:2315761:1 11/11 None
Y39G10AR.8 ENSEMBL cdna:known chromosome:CEL140:I:2313271:2315985:1 gene:Y39G10AR.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2311929-2315761(+) 97.13 100.0
Public Domain and Genome References
Gene Title
Ensembl Y39G10AR.8
Entrez Gene 171792 Entrez gene
SwissProt Q95XR0 EMBL-EBI
WormBase CE27254 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17928_AT eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative at
ATH1-121501:254666_AT eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative at
MU11KSUBB:MSA.34975.0_S_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MG-U74AV2:103674_F_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MOE430A:1417210_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MOUSE430A_2:1417210_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MOUSE430_2:1417210_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MOE430B:1431642_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MOUSE430_2:1431642_AT eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked mm
MOUSE430_2:1457945_AT Eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y), mRNA mm
MOE430B:1457945_AT Eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y), mRNA mm
MU19KSUBC:TC39182_AT Eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_490960 Y39G10AR.8 [Caenorhabditis elegans] gb|AAK39619.1| Hypothetical protein Y39G10AR.8 [Caenorhabditis elegans] 0.0
blast CAE60299 Hypothetical protein CBG03884 [Caenorhabditis briggsae] 0.0
blast XP_787081 PREDICTED: similar to eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa [Strongylocentrotus purpuratus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 6.4E-11
Pfam IPR004226 EMBL-EBI Tubulin binding cofactor A 6.4E-13
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 5.0E-34
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 6.4E-11
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 5.0E-34
Sequence
Target Sequence 
>C. ELEGANS:176446_S_AT
ggagtcgccggaggaacattgaccaagggaattctgcgcgtcggacaagaaatcgaagtt
cgcccgggaatcgtctcaaaaaccgcgaccggtcaactccaatgccgtccgatcttctcc
cgtatcgactctctgtttgccgaaaagaaccagctggaatacgccgttcccggcggtctc
atcggtgttggaacgaagatcgacccgacactctgccgtggagatcgactcgtcggtcac
atcctcggagccgttggcactcttccagatatcttcatcgaaattgaaatctccttctat
cttctgagaagattgctcggagttcgtactgaaggaaagaagaagggagccaaggtgcag
aagcttgtcaaggaagagactttgctggtgaacattggatcgttgtcaacgggaggaaga
gttactgctgtgaagggagacgccgcgaagatcagattaaacgatcctatttgcacagag
gttggagagaagattgccatgtcacgtcgtttcgagaagagttggcgcctgattggctgg
ggaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGTCGCCGGAGGAACATTGACCA 90 517 1414 Antisense
GACCAAGGGAATTCTGCGCGTCGGA 224 385 1434 Antisense
AGAACCAGCTGGAATACGCCGTTCC 268 73 1559 Antisense
GCGGTCTCATCGGTGTTGGAACGAA 588 291 1586 Antisense
TGGAACGAAGATCGACCCGACACTC 425 547 1602 Antisense
GGAGATCGACTCGTCGGTCACATCC 264 519 1633 Antisense
GTTGGCACTCTTCCAGATATCTTCA 3 437 1666 Antisense
ATTGAAATCTCCTTCTATCTTCTGA 456 5 1696 Antisense
GATTGCTCGGAGTTCGTACTGAAGG 589 433 1724 Antisense
AGAAGATTGCCATGTCACGTCGTTT 279 75 1901 Antisense
GTTGGCGCCTGATTGGCTGGGGAAC 517 435 1934 Antisense
Sequence Source Affymetrix Proprietary Database