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[176445_at]

Description Y54F10AM.L /REP_DB=WormBase Gene ID /WP=CE26180 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_highest_nonorm > 50      6grp_total > 05      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 414
1 2 3 4 range
A 799.7
(3)
P 		647.2
(0.1)
P 		489.4
(-3)
P 		506.3
(-2.7)
P 		310
B 714.5
(3)
P 		635.5
(1.2)
P 		451.7
(-3)
P 		638.7
(1.3)
P 		263
C 781.6
(3)
P 		645.6
(0.9)
P 		391.4
(-2.9)
P 		386
(-3)
P 		396
avr 765.3
(3) 		642.8
(0.7) 		444.2
(-3) 		510.3
(-1.8) 		321

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 703
1 2 3 4 5 6 range
A 904.9
(3)
P 		852.3
(2.5)
P 		318
(-2.7)
P 		334.1
(-2.6)
P 		434.8
(-1.6)
P 		289.9
(-3)
P 		615
B 740.1
(2.4)
P 		791.3
(3)
P 		356.1
(-1.9)
P 		333
(-2.2)
P 		441.4
(-0.9)
P 		259.4
(-3)
P 		532
C 899.6
(3)
P 		753.3
(1.7)
P 		473.1
(-0.7)
P 		308
(-2.1)
P 		467.4
(-0.7)
P 		202.1
(-3)
P 		698
avr 848.2
(3) 		799
(2.5) 		382.4
(-1.7) 		325
(-2.3) 		447.9
(-1) 		250.5
(-3) 		598
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 226
1 2 3 4 range
A 291.2
(3)
P 		140.5
(-1.2)
P 		127.9
(-1.5)
P 		74.1
(-3)
P 		217
B 227.8
(3)
P 		157
(-0.5)
P 		104.7
(-3)
P 		136.1
(-1.5)
P 		123
C 258.7
(3)
P 		185.6
(0.7)
P 		136.4
(-0.8)
P 		65.1
(-3)
P 		194
avr 259.2
(3) 		161
(-0.5) 		123
(-1.9) 		91.8
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 166.8
(-0.5)
P 		303.1
(3)
P 		69.3
(-3)
P 		84.7
(-2.6)
P 		131.2
(-1.4)
P 		70.6
(-3)
P 		234
B 257.5
(2.8)
P 		263.4
(3)
P 		86.5
(-3)
P 		88.2
(-2.9)
P 		179.3
(0.1)
P 		96.7
(-2.7)
P 		177
C 333.4
(3)
P 		229.8
(0.7)
P 		120.4
(-1.7)
P 		61.9
(-3)
P 		151.3
(-1)
P 		67.7
(-2.9)
P 		272
avr 252.6
(2.6) 		265.4
(3) 		92.1
(-2.6) 		78.3
(-3) 		153.9
(-0.6) 		78.3
(-3) 		187
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176445_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18950
Sequence Type Exemplar sequence
Representative Public ID Y54F10AM.L NCBI
Target Description Y54F10AM.L /REP_DB=WormBase Gene ID /WP=CE26180 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027735(11), NM_065177(11), NM_001027736(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027735 NCBI Caenorhabditis elegans mammalian FE65 Homolog family member (feh-1) (feh-1) mRNA, complete cds. 11/11 None
NM_065177 NCBI Caenorhabditis elegans mammalian FE65 Homolog family member (feh-1) (feh-1) mRNA, complete cds. 11/11 A 
NM_001027736 NCBI Caenorhabditis elegans mammalian FE65 Homolog family member (feh-1) (feh-1) mRNA, complete cds. 11/11 None
GENEFINDER00000034926 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2549921:2560393:1 11/11 None
SNAP00000034924 ENSEMBL cdna:SNAP chromosome:CEL140:III:2554125:2566136:1 11/11 None
Y54F10AM.2b ENSEMBL cdna:known chromosome:CEL140:III:2549916:2560662:1 gene:Y54F10AM.2 11/11 A 
Y54F10AM.2c ENSEMBL cdna:known chromosome:CEL140:III:2554948:2560657:1 gene:Y54F10AM.2 11/11 A 
Y54F10AM.2a ENSEMBL cdna:known chromosome:CEL140:III:2554948:2560323:1 gene:Y54F10AM.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2554947-2559516(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y54F10AM.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84831 Mammalian fe65 homolog protein 1, isoform b [Caenorhabditis elegans] ref|NP_001022906.1| mammalian FE65 Homolog family member (feh-1) [Caenorhabditis elegans] 0.0
blast AAK29940 Mammalian fe65 homolog protein 1, isoform a [Caenorhabditis elegans] ref|NP_497578.3| mammalian FE65 Homolog family member (feh-1) [Caenorhabditis elegans] 0.0
blast CAE65009 Hypothetical protein CBG09847 [Caenorhabditis briggsae] 0.0
blast CAC87812 FEH-1 protein [Caenorhabditis elegans] 0.0
blast AAN84832 Mammalian fe65 homolog protein 1, isoform c [Caenorhabditis elegans] ref|NP_001022907.1| mammalian FE65 Homolog family member (feh-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 8.1E-28
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-6
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 4.4E-27
Pfam IPR006020 EMBL-EBI Phosphotyrosine interaction domain 9.0E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000034926 2 352-374,381-403
Sequence
Target Sequence 
>C. ELEGANS:176445_AT
gaccatcaagtcttcatgctatcgaatcaggagaaaagagaatcgtgcgaagtgaggggc
ttactgcaccgattgatgagccacgaaaagtgatccgttgccattttctcggagtcacac
aggttccaaaggcgactggaatcgagattctcaacgaggcagtcgatcgattagtgtcac
aagttcggtcggaaagatggattctggcggatgtatcaattgctccgtcgactattgcaa
ttgttgaagtgaatggacaacaaattgccgagtgcagagttagatatctttcatttttgg
gaattggacgagatgtcaaacactgtgccttcataatgcaaacctcatcggaaagcttca
tgtgctacgtcttccacgtcgaaccgaacgcagccgccatggcaaaaatggtcgaggctg
cttgtaagcttcgataccaaaaagtgctcgacgcccactcatcatcccgtcatcactcgg
gaatgtcgattcatggacaacatccaccatccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCATCAAGTCTTCATGCTATCGA 389 383 1556 Antisense
GCGAAGTGAGGGGCTTACTGCACCG 72 287 1602 Antisense
TACTGCACCGATTGATGAGCCACGA 80 645 1617 Antisense
GATCCGTTGCCATTTTCTCGGAGTC 4 417 1647 Antisense
GGATGTATCAATTGCTCCGTCGACT 149 515 1764 Antisense
GAGATGTCAAACACTGTGCCTTCAT 644 395 1865 Antisense
AAAGCTTCATGTGCTACGTCTTCCA 245 121 1907 Antisense
CGAACGCAGCCGCCATGGCAAAAAT 202 253 1940 Antisense
GGTCGAGGCTGCTTGTAAGCTTCGA 103 501 1965 Antisense
TCATCATCCCGTCATCACTCGGGAA 430 619 2014 Antisense
TTCATGGACAACATCCACCATCCAC 523 681 2045 Antisense
Sequence Source Affymetrix Proprietary Database