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[176440_at]

Description Y45G5AL.A /REP_DB=WormBase Gene ID /WP=CE26534 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      4grp_highest_8_8 > 04      4grp_high_8_8 > 29      6grp_highest_nonorm > 91      6grp_total > 49      4grp_total > 34      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 311.1
(-2.7)
P 		479.2
(3)
P 		302.9
(-3)
P 		429.7
(1.3)
P 		176
B 278.8
(-3)
P 		313.7
(-0.8)
P 		332.5
(0.3)
P 		375.1
(3)
P 		96
C 251.6
(-3)
P 		428.6
(3)
P 		270.7
(-2.4)
P 		339.9
(-0)
P 		177
avr 280.5
(-3) 		407.2
(3) 		302
(-2) 		381.6
(1.8) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 201.1
(-0.4)
P 		180.5
(-1.4)
P 		212.4
(0.1)
P 		195.8
(-0.7)
P 		143.7
(-3)
P 		278
(3)
P 		134
B 248.2
(3)
P 		193.6
(0.7)
P 		119.8
(-2.5)
P 		108.3
(-3)
P 		181.6
(0.1)
P 		188.6
(0.4)
P 		140
C 229.1
(1.7)
P 		185.2
(-2.4)
P 		213
(0.2)
P 		178.6
(-3)
P 		243
(3)
P 		190.8
(-1.9)
P 		64
avr 226.1
(3) 		186.4
(-0.7) 		181.7
(-1.1) 		160.9
(-3) 		189.4
(-0.4) 		219.1
(2.4) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 113.3
(3)
P 		104
(1.9)
P 		79.2
(-1.1)
P 		62.9
(-3)
P 		50
B 88.9
(3)
P 		77.5
(-2.8)
P 		77.1
(-3)
P 		79.9
(-1.6)
P 		12
C 83.3
(-0.6)
P 		123.2
(3)
P 		94.4
(0.4)
P 		57.3
(-3)
P 		66
avr 95.2
(1.9) 		101.6
(3) 		83.6
(-0.1) 		66.7
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 37.1
(-3)
P 		64.2
(2.3)
P 		46.2
(-1.2)
P 		49.7
(-0.5)
P 		43.4
(-1.8)
P 		67.7
(3)
P 		31
B 86.4
(3)
P 		64.4
(0.7)
P 		29.1
(-3)
P 		28.7
(-3)
P 		73.8
(1.7)
P 		70.3
(1.3)
P 		58
C 84.9
(3)
P 		56.5
(-0.5)
P 		54.2
(-0.8)
P 		35.9
(-3)
P 		78.6
(2.2)
P 		63.9
(0.4)
P 		49
avr 69.5
(3) 		61.7
(1.5) 		43.2
(-2) 		38.1
(-3) 		65.3
(2.2) 		67.3
(2.6) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176440_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19538
Sequence Type Exemplar sequence
Representative Public ID Y45G5AL.A NCBI
Target Description Y45G5AL.A /REP_DB=WormBase Gene ID /WP=CE26534 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171445, NM_171924
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171924 NCBI Caenorhabditis elegans Y45G5AL.1a (Y45G5AL.1) mRNA, complete cds. A 
NM_171445 NCBI Caenorhabditis elegans Y45G5AL.1b (Y45G5AL.1) mRNA, complete cds. A 
Y45G5AL.1a.2 ENSEMBL cdna:known chromosome:CEL140:V:4144317:4147315:1 gene:Y45G5AL.1 None
Y45G5AL.1a.1 ENSEMBL cdna:known chromosome:CEL140:V:4144319:4147372:1 gene:Y45G5AL.1 None
Y45G5AL.1b ENSEMBL cdna:known chromosome:CEL140:V:4144320:4147078:1 gene:Y45G5AL.1 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000031149 4/11 Cross Hyb Matching Probes None
GENEFINDER00000031152 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4139689-4144857(+) 83.88 100.0
Public Domain and Genome References
Ensembl Y45G5AL.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741521 Y45G5AL.1b [Caenorhabditis elegans] gb|AAL32246.1| Hypothetical protein Y45G5AL.1b [Caenorhabditis elegans] 1.0E-156
blast NP_741522 Y45G5AL.1a [Caenorhabditis elegans] gb|AAL32245.1| Hypothetical protein Y45G5AL.1a [Caenorhabditis elegans] 1.0E-155
blast NP_741521 Y45G5AL.1b [Caenorhabditis elegans] gb|AAL32246.1| Hypothetical protein Y45G5AL.1b [Caenorhabditis elegans] 1.0E-153
blast NP_741522 Y45G5AL.1a [Caenorhabditis elegans] gb|AAL32245.1| Hypothetical protein Y45G5AL.1a [Caenorhabditis elegans] 1.0E-153
Sequence
Target Sequence 
>C. ELEGANS:176440_AT
aacccgggattctacatagttcttgtgatgattcccgcgtatttcattaatgttctgtca
attgttgctctattcatggatattaataatcgatcggaaaagttcacagttggcatgaca
aatatcatgtcgatgagctttatcctcgtgattttagcagaggatttgccgaagacgaaa
aatttaccgattttggattcatctctaaacatggatggtattttccgcctattctgtgga
tgctgcggaatacaaggcgaagaagagccgccatactcacagctccgccacgattcggtc
accccgattcgtgatccaccactccagcagggaaatcgccaggcgacgaatggagcacat
cagcataatcctcttcagcatcaaggattcgtgaatccggttcacgaatataattacgac
gccaccgatgggccgaacatgtatccatcggaccatcatggaatgcattcggactcggcg
tcaccaccatccggtgattttgatgaggagatcgttgaggatgagggccgtgaaaaggag
cagcttgacaatattgtcgagagcacgcagcaccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCCGGGATTCTACATAGTTCTTG 55 147 859 Antisense
TAGTTCTTGTGATGATTCCCGCGTA 256 651 875 Antisense
GATTCCCGCGTATTTCATTAATGTT 686 431 888 Antisense
GTCGATGAGCTTTATCCTCGTGATT 85 475 987 Antisense
TTTCCGCCTATTCTGTGGATGCTGC 33 669 1080 Antisense
TCCAGCAGGGAAATCGCCAGGCGAC 379 589 1181 Antisense
GCACATCAGCATAATCCTCTTCAGC 219 317 1213 Antisense
CGCCACCGATGGGCCGAACATGTAT 661 257 1278 Antisense
GAACATGTATCCATCGGACCATCAT 219 351 1293 Antisense
CATGGAATGCATTCGGACTCGGCGT 294 209 1315 Antisense
TATTGTCGAGAGCACGCAGCACCTA 80 659 1410 Antisense
Sequence Source Affymetrix Proprietary Database