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[176439_at]

Description F54H12.5 /REP_DB=WormBase Gene ID /WP=CE00547 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 79      4grp_total > 01      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 1
(-3)
A 		1.4
(-2.9)
A 		1.3
(-3)
A 		48.7
(3)
P 		48
B 3.9
(-2.4)
A 		1.9
(-3)
A 		11.1
(-0.1)
A 		20.7
(3)
A 		19
C 3.9
(-2.1)
A 		0.9
(-3)
A 		1.6
(-2.8)
A 		21.9
(3)
A 		21
avr 2.9
(-2.7) 		1.4
(-3) 		4.7
(-2.3) 		30.4
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 21.2
(0.1)
A 		0.9
(-3)
A 		19.2
(-0.2)
A 		10.8
(-1.5)
A 		40.2
(3)
A 		37.9
(2.6)
A 		39
B 34.1
(0.4)
A 		21
(-1.7)
A 		13.1
(-3)
A 		19.7
(-1.9)
A 		44.5
(2.1)
P 		49.8
(3)
P 		37
C 25.9
(-0.9)
A 		34.8
(-0.2)
P 		0.5
(-3)
A 		22.9
(-1.2)
A 		27.3
(-0.8)
A 		73.5
(3)
P 		73
avr 27.1
(-0.7) 		18.9
(-1.9) 		10.9
(-3) 		17.8
(-2) 		37.3
(0.7) 		53.7
(3) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 0.3
(-3)
A 		0.3
(-3)
A 		0.3
(-3)
A 		7.1
(3)
P 		7
B 1.2
(-1.9)
A 		0.5
(-3)
A 		2.6
(0.2)
A 		4.4
(3)
A 		4
C 1.3
(-1.2)
A 		0.3
(-3)
A 		0.6
(-2.5)
A 		3.7
(3)
A 		3
avr 0.9
(-2.4) 		0.4
(-3) 		1.2
(-2) 		5.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 3.9
(-1.2)
A 		0.3
(-3)
A 		4.2
(-1)
A 		2.7
(-1.8)
A 		12.1
(3)
A 		9.2
(1.5)
A 		12
B 11.9
(0.4)
A 		7
(-1.5)
A 		3.2
(-3)
A 		5.2
(-2.2)
A 		18.1
(2.8)
P 		18.6
(3)
P 		15
C 9.6
(-0.7)
A 		10.6
(-0.4)
P 		0.1
(-3)
A 		4.6
(-1.9)
A 		8.8
(-0.9)
A 		24.6
(3)
P 		25
avr 8.5
(-0.6) 		6
(-1.6) 		2.5
(-3) 		4.2
(-2.3) 		13
(1.2) 		17.5
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176439_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7122
Sequence Type Exemplar sequence
Representative Public ID F54H12.5 NCBI
Target Description F54H12.5 /REP_DB=WormBase Gene ID /WP=CE00547 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066335(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066335 NCBI Caenorhabditis elegans F54H12.5 (F54H12.5) mRNA, complete cds. 11/11 None
SNAP00000022461 ENSEMBL cdna:SNAP chromosome:CEL140:III:7966566:7969099:-1 9/11 None
GENEFINDER00000022466 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7966566:7969099:-1 11/11 None
F54H12.5 ENSEMBL cdna:known chromosome:CEL140:III:7966566:7972026:-1 gene:F54H12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7966563-7972024(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54H12.5
Entrez Gene 186269 Entrez gene
SwissProt P34459 EMBL-EBI
WormBase CE00547 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28052 Hypothetical protein F54H12.5 [Caenorhabditis elegans] ref|NP_498736.1| F54H12.5 [Caenorhabditis elegans] sp|P34459|YMD5_CAEEL Hypothetical protein F54H12.5 in chromosome III 0.0
blast AAA28052 Hypothetical protein F54H12.5 [Caenorhabditis elegans] ref|NP_498736.1| F54H12.5 [Caenorhabditis elegans] sp|P34459|YMD5_CAEEL Hypothetical protein F54H12.5 in chromosome III 1.0E-163
blast CAE75032 Hypothetical protein CBG22939 [Caenorhabditis briggsae] 1.0E-107
blast CAE75032 Hypothetical protein CBG22939 [Caenorhabditis briggsae] 2.0E-41
blast AAF39964 Hypothetical protein T02H6.5 [Caenorhabditis elegans] ref|NP_493792.1| T02H6.5 [Caenorhabditis elegans] 1.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 9.6E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498736.1 1 810-832
F54H12.5 1 810-832
SNAP00000022461 1 416-438
GENEFINDER00000022466 1 500-522
Sequence
Target Sequence 
>C. ELEGANS:176439_AT
gacaagttcttctcttatgcggattcaaaaggaaatgattttattctaattcatcataag
gctcaatggatttatcgaatcaattatcaattgtatttgaacaatcataacatgtttttc
aaagtttttcatctgagaactcctgatggtaaactgcaatttgataactttgagcacgct
gatgagtatggacttgtcacaagatattcaaattctgatggtcgctatcggtattattat
tcgctgttcagtgagatgtccggtttgacaacttattgtgttcatgatcagtatattgat
ggacgtttgatgattttgggaagacagcgcattgtgccagtcaaaagttttactgatgaa
ctccgtcaaatgattaatatcgagaaaggggaggatactgatattccaacaatgcttcgt
cttcttttttattgctcattgctctatttattcattttcgtgacagtacaaatgattcca
gctcgagagataagtattatgaaggatttgcatcacattggtcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAGTTCTTCTCTTATGCGGATT 271 365 2032 Antisense
GAACTCCTGATGGTAAACTGCAATT 579 347 2168 Antisense
GAGCACGCTGATGAGTATGGACTTG 664 389 2203 Antisense
AATTCTGATGGTCGCTATCGGTATT 82 177 2242 Antisense
ATCGGTATTATTATTCGCTGTTCAG 510 35 2258 Antisense
GCTGTTCAGTGAGATGTCCGGTTTG 450 293 2274 Antisense
GGAAGACAGCGCATTGTGCCAGTCA 464 531 2350 Antisense
CGTCTTCTTTTTTATTGCTCATTGC 30 247 2449 Antisense
TGCTCATTGCTCTATTTATTCATTT 348 581 2464 Antisense
GACAGTACAAATGATTCCAGCTCGA 236 369 2493 Antisense
GAAGGATTTGCATCACATTGGTCGT 320 335 2532 Antisense
Sequence Source Affymetrix Proprietary Database