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[176437_at]

Description C30F12.3 /REP_DB=WormBase Gene ID /WP=CE16883 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 56      4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_highest_nonorm > 85      6grp_total > 46      4grp_total > 04      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4172
1 2 3 4 range
A 1114.9
(-1.3)
P 		145
(-3)
P 		138.6
(-3)
P 		3563.6
(3)
P 		3425
B 963
(-1.5)
P 		226.4
(-2.8)
P 		118.5
(-3)
P 		3452.4
(3)
P 		3334
C 1023.7
(-1.8)
P 		246.3
(-2.9)
P 		167.9
(-3)
P 		4290.7
(3)
P 		4123
avr 1033.9
(-1.5) 		205.9
(-2.9) 		141.7
(-3) 		3768.9
(3) 		3627

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 622
1 2 3 4 5 6 range
A 713.7
(1.2)
P 		728.9
(1.5)
P 		801.2
(3)
P 		728.4
(1.5)
P 		513.1
(-3)
P 		795.9
(2.9)
P 		288
B 852.8
(-0.1)
P 		950.7
(1.1)
P 		782
(-0.9)
P 		599.5
(-3)
P 		638.5
(-2.5)
P 		1116.3
(3)
P 		517
C 795.8
(0.2)
P 		915
(1.4)
P 		581.1
(-2.1)
P 		706.6
(-0.8)
P 		494.7
(-3)
P 		1061.4
(3)
P 		567
avr 787.4
(0.2) 		864.9
(1.3) 		721.4
(-0.7) 		678.2
(-1.2) 		548.8
(-3) 		991.2
(3) 		442
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 708
1 2 3 4 range
A 405.9
(1.6)
P 		31.5
(-3)
P 		36.2
(-2.9)
P 		521.6
(3)
P 		490
B 307.1
(-0.6)
P 		55.9
(-2.8)
P 		27.5
(-3)
P 		735.7
(3)
P 		708
C 338.9
(-0.5)
P 		70.8
(-2.9)
P 		58.5
(-3)
P 		723.5
(3)
P 		665
avr 350.6
(0) 		52.7
(-2.9) 		40.7
(-3) 		660.3
(3) 		620

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 285
1 2 3 4 5 6 range
A 131.6
(-3)
P 		259.2
(3)
P 		174.5
(-1)
P 		184.7
(-0.5)
P 		154.8
(-1.9)
P 		193.8
(-0.1)
P 		128
B 296.7
(0.2)
P 		316.4
(0.7)
P 		189.9
(-2.3)
P 		158.8
(-3)
P 		259.4
(-0.7)
P 		416.3
(3)
P 		258
C 295
(1.3)
P 		279.2
(0.9)
P 		147.9
(-2.8)
P 		142.1
(-3)
P 		160.1
(-2.5)
P 		355.3
(3)
P 		213
avr 241.1
(-0) 		284.9
(1.6) 		170.8
(-2.7) 		161.9
(-3) 		191.4
(-1.9) 		321.8
(3) 		160
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176437_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.956
Sequence Type Exemplar sequence
Representative Public ID C30F12.3 NCBI
Target Description C30F12.3 /REP_DB=WormBase Gene ID /WP=CE16883 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059586(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059586 NCBI Caenorhabditis elegans C30F12.3 (C30F12.3) mRNA, complete cds. 11/11 None
SNAP00000017405 ENSEMBL cdna:SNAP chromosome:CEL140:I:6978579:6979930:-1 11/11 None
GENEFINDER00000017420 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6978579:6981801:-1 11/11 None
C30F12.3 ENSEMBL cdna:known chromosome:CEL140:I:6978521:6979930:-1 gene:C30F12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6978509-6979861(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C30F12.3
Entrez Gene 172428 Entrez gene
SwissProt Q95YE0 EMBL-EBI
WormBase CE16883 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85449 Hypothetical protein C30F12.3 [Caenorhabditis elegans] ref|NP_491987.1| C30F12.3 [Caenorhabditis elegans] 1.0E-135
blast AAK85449 Hypothetical protein C30F12.3 [Caenorhabditis elegans] ref|NP_491987.1| C30F12.3 [Caenorhabditis elegans] 1.0E-123
blast CAE60341 Hypothetical protein CBG03933 [Caenorhabditis briggsae] 1.0E-115
blast CAE60341 Hypothetical protein CBG03933 [Caenorhabditis briggsae] 1.0E-106
blast AAK85450 Hypothetical protein C30F12.4 [Caenorhabditis elegans] ref|NP_491988.1| C30F12.4 [Caenorhabditis elegans] 1.0E-20
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491987.1 4 63-85,95-117,122-144,186-208
C30F12.3 4 63-85,95-117,122-144,186-208
SNAP00000017405 4 63-85,95-117,122-144,186-208
GENEFINDER00000017420 4 271-293,303-325,330-352,394-416
Sequence
Target Sequence 
>C. ELEGANS:176437_AT
agttttcgaactttggcatctcattgtatccatcttagcatcggaattggtcgaaagagc
ggatgtaactggagcagctattcgatttttcgctggtgtcttcattgcaggtacagtact
ctgggcgattttcgcgcaaagagcagaatttcttgtgccgtatcttctgttacagggtgc
tgggcttgccatcggccttatctttttcgtttcattcatgtatatcggtctttttggaga
caaaaaagttggcacaacagtccttggatcatacggtattccagtaaatccacgtgatga
gacagcgtatttcaattatgctgcctggttgatggctggatcattcgcgattgtcatagt
ccttcaaatttggatgatgatgattgttgtcgcgtgttggagatttttcagagacaaacg
taataacgagaagatttataaggaagtggtgacggtgaagaagattcaattgctaacccg
aatgagaatatccctttccgcggaagctcttggttccgaatcatcacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTTTCGAACTTTGGCATCTCATT 446 63 228 Antisense
GGCATCTCATTGTATCCATCTTAGC 49 537 242 Antisense
ATTTTTCGCTGGTGTCTTCATTGCA 474 15 312 Antisense
TACAGTACTCTGGGCGATTTTCGCG 287 643 339 Antisense
TCTTGTGCCGTATCTTCTGTTACAG 325 619 378 Antisense
CCTTATCTTTTTCGTTTCATTCATG 183 269 423 Antisense
GTTGGCACAACAGTCCTTGGATCAT 159 437 475 Antisense
GCGTATTTCAATTATGCTGCCTGGT 616 291 532 Antisense
CATTCGCGATTGTCATAGTCCTTCA 162 217 569 Antisense
GAATGAGAATATCCCTTTCCGCGGA 616 331 707 Antisense
AGCTCTTGGTTCCGAATCATCACAT 426 79 732 Antisense
Sequence Source Affymetrix Proprietary Database