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[176434_s_at]

Description Y105E8B.TT /REP_DB=WormBase Gene ID /WP=CE25702 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_high1_10_10 > 71      6grp_total > 80      4grp_total > 42      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 72.1
(-3)
A 		301.5
(3)
P 		204.3
(0.5)
P 		164.1
(-0.6)
A 		229
B 17.2
(-3)
A 		313
(3)
P 		178.8
(0.3)
P 		145
(-0.4)
P 		296
C 46.9
(-3)
A 		334.6
(3)
P 		203.5
(0.3)
P 		138.3
(-1.1)
A 		288
avr 45.4
(-3) 		316.4
(3) 		195.5
(0.3) 		149.1
(-0.7) 		271

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 82.3
(-3)
A 		207.5
(0.2)
P 		177
(-0.6)
P 		315.6
(3)
P 		179.4
(-0.5)
P 		181.8
(-0.4)
P 		233
B 174.7
(-2)
P 		184
(-1.1)
P 		164.2
(-2.9)
P 		230.6
(3)
P 		191.3
(-0.5)
P 		163.2
(-3)
P 		67
C 148.8
(-3)
P 		191.7
(2.8)
P 		193.4
(3)
P 		185
(1.9)
P 		191
(2.7)
P 		170.8
(-0)
P 		45
avr 135.3
(-3) 		194.4
(0.3) 		178.2
(-0.6) 		243.7
(3) 		187.2
(-0.1) 		171.9
(-1) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 26.2
(-2.7)
A 		65.5
(3)
P 		53.4
(1.3)
P 		24
(-3)
A 		42
B 5.5
(-3)
A 		77.3
(3)
P 		41.4
(0)
P 		30.9
(-0.9)
P 		72
C 15.5
(-3)
A 		96.2
(3)
P 		70.9
(1.1)
P 		23.3
(-2.4)
A 		81
avr 15.7
(-3) 		79.7
(3) 		55.2
(0.7) 		26.1
(-2) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 15.2
(-3)
A 		73.8
(2.4)
P 		38.5
(-0.8)
P 		80
(3)
P 		54.1
(0.6)
P 		44.3
(-0.3)
P 		65
B 60.8
(0.3)
P 		61.3
(0.4)
P 		39.9
(-3)
P 		61.1
(0.4)
P 		77.7
(3)
P 		60.8
(0.3)
P 		38
C 55.2
(1.4)
P 		58.5
(2.2)
P 		49.2
(-0.1)
P 		37.2
(-3)
P 		61.8
(3)
P 		57.2
(1.9)
P 		25
avr 43.7
(-2.7) 		64.5
(3) 		42.5
(-3) 		59.4
(1.6) 		64.5
(3) 		54.1
(0.2) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176434_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18820
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.TT NCBI
Target Description Y105E8B.TT /REP_DB=WormBase Gene ID /WP=CE25702 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061147(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061147 NCBI Caenorhabditis elegans Y105E8B.9 (Y105E8B.9) mRNA, complete cds. 11/11 None
SNAP00000011738 ENSEMBL cdna:SNAP chromosome:CEL140:I:14676378:14686602:-1 11/11 None
GENEFINDER00000011746 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14683825:14690681:-1 11/11 None
Y105E8B.9 ENSEMBL cdna:known chromosome:CEL140:I:14683783:14690681:-1 gene:Y105E8B.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14683755-14690612(-) 99.84 99.84
Public Domain and Genome References
Gene Title
Ensembl Y105E8B.9
Entrez Gene 173326 Entrez gene
SwissProt Q95Q32 EMBL-EBI
WormBase CE28788 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582365_S_AT glucuronidase, beta cfa
CANINE:1582364_AT glucuronidase, beta cfa
CANINE_2:CFA.3694.1.A1_S_AT glucuronidase, beta cfa
DROSOPHILA_2:1624466_A_AT dm
DROSGENOME1:153526_AT dm
CHICKEN:GGAAFFX.12983.1.S1_S_AT similar to beta-glucuronidase (EC 3.2.1.31) allele B precursor - mouse gga
HG-FOCUS:202605_AT glucuronidase, beta hs
HG-U133A_2:202605_AT glucuronidase, beta hs
HG-U133A:202605_AT glucuronidase, beta hs
HG-U133_PLUS_2:202605_AT glucuronidase, beta hs
HUGENEFL:M15182_AT glucuronidase, beta hs
U133_X3P:G4504222_3P_AT glucuronidase, beta hs
HG-U95AV2:33308_AT glucuronidase, beta hs
HG-U133_PLUS_2:230125_AT Glucuronidase, beta hs
HG-U133B:230125_AT Glucuronidase, beta hs
HU35KSUBA:R71205_AT Glucuronidase, beta hs
U133_X3P:HS.183868.1.A1_3P_AT Glucuronidase, beta hs
MU11KSUBA:M63836_S_AT glucuronidase, beta mm
MG-U74AV2:97538_AT glucuronidase, beta mm
MG-U74CV2:171015_R_AT glucuronidase, beta mm
MOE430A:1430332_A_AT glucuronidase, beta mm
MOUSE430A_2:1430332_A_AT glucuronidase, beta mm
MOUSE430_2:1430332_A_AT glucuronidase, beta mm
MOE430A:1448124_AT glucuronidase, beta mm
MOUSE430A_2:1448124_AT glucuronidase, beta mm
MOUSE430_2:1448124_AT glucuronidase, beta mm
MU19KSUBA:TC19299_AT Glucuronidase, beta, mRNA (cDNA clone IMAGE:3675986) mm
MU19KSUBB:TC26529_S_AT Glucuronidase, beta, mRNA (cDNA clone IMAGE:3675986) mm
MU19KSUBB:TC30096_S_AT Glucuronidase, beta, mRNA (cDNA clone IMAGE:3675986) mm
RG-U34A:M13962MRNA#2_AT glucuronidase, beta rn
RAE230A:1367901_AT glucuronidase, beta rn
RAT230_2:1367901_AT glucuronidase, beta rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC70111 Hypothetical protein Y105E8B.9 [Caenorhabditis elegans] ref|NP_493548.1| Y105E8B.9 [Caenorhabditis elegans] 0.0
blast CAE72612 Hypothetical protein CBG19804 [Caenorhabditis briggsae] 0.0
blast CAE72612 Hypothetical protein CBG19804 [Caenorhabditis briggsae] 1.0E-170
blast CAC70111 Hypothetical protein Y105E8B.9 [Caenorhabditis elegans] ref|NP_493548.1| Y105E8B.9 [Caenorhabditis elegans] 1.0E-163
blast CAE72612 Hypothetical protein CBG19804 [Caenorhabditis briggsae] 1.0E-157
blast AAZ03639 beta-glucuronidase [Sus scrofa] 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR006103 EMBL-EBI Glycoside hydrolase, family 2, TIM barrel domain 2.3E-62
Pfam IPR006102 EMBL-EBI Glycoside hydrolase family 2, immunoglobulin-like beta-sandwich domain 3.8E-13
Pfam IPR006104 EMBL-EBI Glycoside hydrolase, family 2, sugar binding 2.7E-21
Pfam IPR006104 EMBL-EBI Glycoside hydrolase, family 2, sugar binding 2.1E-6
Pfam IPR006103 EMBL-EBI Glycoside hydrolase, family 2, TIM barrel domain 1.8E-53
Pfam IPR006103 EMBL-EBI Glycoside hydrolase, family 2, TIM barrel domain 2.4E-52
Sequence
Target Sequence 
>C. ELEGANS:176434_S_AT
acaagaaccatccatcagtcatcgcgtggtctctggcaaatgagccgcagactatgaaga
aggagagtaggaattatttcaaaactctggtggacactgctcacggaatcgatcgtactc
gtccggtgactactgtgtatggtccgacgaacttcgataatgatcaaactgcggatttga
tggatttcatttgtgttaatcgatactacggatggtatattgatatgggctacatcccct
ggatcaatcaaagtgtatactgggacatctccctttggcgtgaaaccttccataaaccaa
tcatagtaaccgagtacggagccgacagtattcctggattgaatcaagaaccatctgttg
atttctcggaacaatatcaaaatgaagtgattcaggagacacatcatgcatttgatgcat
tggttaaagatcatacaatcactggagaaatgatttggaatttcgcagattttatgactg
gaatgaccaccacacaagccgtcggaaatcacaaaggagtcttcacgaggtctcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAGAACCATCCATCAGTCATCGC 589 113 1889 Antisense
GCGTGGTCTCTGGCAAATGAGCCGC 220 293 1912 Antisense
TCTGGTGGACACTGCTCACGGAATC 331 607 1974 Antisense
CTCACGGAATCGATCGTACTCGTCC 91 229 1988 Antisense
TACTCGTCCGGTGACTACTGTGTAT 143 647 2004 Antisense
TGATATGGGCTACATCCCCTGGATC 636 563 2109 Antisense
GTATACTGGGACATCTCCCTTTGGC 183 453 2143 Antisense
TCCCTTTGGCGTGAAACCTTCCATA 426 569 2158 Antisense
GTACGGAGCCGACAGTATTCCTGGA 688 457 2202 Antisense
GAACCATCTGTTGATTTCTCGGAAC 419 345 2236 Antisense
AAAGGAGTCTTCACGAGGTCTCGTC 63 121 2401 Antisense
Sequence Source Affymetrix Proprietary Database