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[176433_at]

Description Y71G12A.C /REP_DB=WormBase Gene ID /WP=CE26249 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 08      4grp_total > 08      6grp_cgc > 10      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.9
(-3)
A 		3.1
(-1.9)
A 		6.5
(1.1)
A 		8.6
(3)
A 		7
B 3.7
(3)
A 		3
(-3)
A 		3.5
(1.3)
A 		3.7
(3)
A 		1
C 3.6
(2.2)
A 		3.9
(3)
A 		1.6
(-3)
A 		2.9
(0.4)
A 		2
avr 3.1
(-3) 		3.3
(-2.4) 		3.9
(-0.6) 		5.1
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 5.6
(3)
A 		2.3
(-1.7)
A 		2.4
(-1.6)
A 		1.7
(-2.6)
A 		1.4
(-3)
A 		2.2
(-1.9)
A 		4
B 2.5
(3)
A 		1.8
(0.4)
A 		0.9
(-3)
A 		2.4
(2.6)
A 		2.3
(2.3)
A 		1.7
(-0)
A 		2
C 2.1
(-2.3)
A 		1.8
(-3)
A 		4.4
(3)
A 		2.5
(-1.4)
A 		2.3
(-1.8)
A 		1.9
(-2.8)
A 		3
avr 3.4
(3) 		2
(-2.6) 		2.6
(-0.2) 		2.2
(-1.8) 		2
(-2.6) 		1.9
(-3) 		2
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 0.7
(-3)
A 		0.7
(-3)
A 		1.7
(3)
A 		1.3
(0.6)
A 		1
B 1.2
(3)
A 		0.7
(-3)
A 		0.8
(-1.8)
A 		0.8
(-1.8)
A 		1
C 1.2
(3)
A 		1.1
(2.1)
A 		0.6
(-2.1)
A 		0.5
(-3)
A 		1
avr 1
(3) 		0.8
(-3) 		1
(3) 		0.9
(0) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1
1 2 3 4 5 6 range
A 1
(3)
A 		0.8
(1)
A 		0.5
(-2)
A 		0.4
(-3)
A 		0.4
(-3)
A 		0.5
(-2)
A 		1
B 0.9
(3)
A 		0.6
(0.4)
A 		0.2
(-3)
A 		0.6
(0.4)
A 		0.9
(3)
A 		0.6
(0.4)
A 		1
C 0.8
(0)
A 		0.6
(-2)
A 		1.1
(3)
A 		0.5
(-3)
A 		0.8
(0)
A 		0.6
(-2)
A 		1
avr 0.9
(3) 		0.7
(-0) 		0.6
(-1.5) 		0.5
(-3) 		0.7
(-0) 		0.6
(-1.5) 		0
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176433_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18722
Sequence Type Exemplar sequence
Representative Public ID Y71G12A.C NCBI
Target Description Y71G12A.C /REP_DB=WormBase Gene ID /WP=CE26249 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058512(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058512 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-305) (tag-305) mRNA, complete cds. 11/11 A 
GENEFINDER00000037836 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1873849:1883473:1 11/11 None
SNAP00000037830 ENSEMBL cdna:SNAP chromosome:CEL140:I:1874178:1883473:1 11/11 None
Y71G12A.3 ENSEMBL cdna:known chromosome:CEL140:I:1874148:1884174:1 gene:Y71G12A.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1879664-1883473(+) 99.8 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-305
Ensembl Y71G12A.3
Entrez Gene 171758 Entrez gene
SwissProt Q9BL53 EMBL-EBI
WormBase CE30571 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.19872.1.A1_AT similar to tubby like protein 4 isoform 1 cfa
CANINE_2:CFAAFFX.1901.1.S1_AT similar to tubby like protein 4 isoform 1 cfa
CANINE_2:CFAAFFX.1902.1.S1_S_AT similar to tubby like protein 4 isoform 1 cfa
CHICKEN:GGA.17140.1.S1_AT tubby like protein 4 gga
CHICKEN:GGAAFFX.24261.1.S1_AT tubby like protein 4 gga
HG-U95B:46496_AT tubby like protein 4 hs
HG-U133_PLUS_2:224170_S_AT tubby like protein 4 hs
HG-U133B:224170_S_AT tubby like protein 4 hs
HG-U133_PLUS_2:218184_AT tubby like protein 4 hs
HG-U133A:218184_AT tubby like protein 4 hs
HG-FOCUS:218184_AT tubby like protein 4 hs
HG-U133A_2:218184_AT tubby like protein 4 hs
HU35KSUBA:AA186729_AT tubby like protein 4 hs
HU35KSUBA:RC_AA609436_AT tubby like protein 4 hs
HU35KSUBD:RC_AA187679_AT tubby like protein 4 hs
U133_X3P:G9858153_3P_A_AT tubby like protein 4 hs
U133_X3P:G12383052_3P_AT tubby like protein 4 hs
HG-U95B:51285_AT tubby like protein 4 hs
HG-U95B:58017_AT tubby like protein 4 hs
HU35KSUBB:RC_H97678_AT Tubby like protein 4 hs
HG-U133_PLUS_2:239742_AT Tubby like protein 4 hs
HG-U133B:239742_AT Tubby like protein 4 hs
HG-U95B:53339_AT Tubby like protein 4 hs
HG-U133_PLUS_2:232372_AT Tubby like protein 4 hs
HG-U133B:232372_AT Tubby like protein 4 hs
HG-U133_PLUS_2:244195_AT Tubby like protein 4 hs
HG-U133B:244195_AT Tubby like protein 4 hs
HG-U95E:69317_AT Tubby like protein 4 hs
HG-U95E:76979_AT Tubby like protein 4 hs
HG-U95D:77500_AT Tubby like protein 4 hs
HG-U95D:79884_AT Tubby like protein 4 hs
U133_X3P:244195_3P_AT Tubby like protein 4 hs
U133_X3P:HS.141971.0.S1_3P_AT Tubby like protein 4 hs
U133_X3P:HS.141971.0.S1_3P_X_AT Tubby like protein 4 hs
MU11KSUBA:AA673986_S_AT tubby like protein 4 mm
MG-U74AV2:160775_AT tubby like protein 4 mm
MOE430A:1448548_AT tubby like protein 4 mm
MOUSE430A_2:1448548_AT tubby like protein 4 mm
MOUSE430_2:1448548_AT tubby like protein 4 mm
MOE430B:1440282_AT tubby like protein 4 mm
MOUSE430_2:1440282_AT tubby like protein 4 mm
MG-U74CV2:135773_AT Tubby like protein 4 (Tulp4), mRNA mm
MU19KSUBA:TC25174_AT Tubby like protein 4 (Tulp4), mRNA mm
MU19KSUBB:TC31681_AT Tubby like protein 4 (Tulp4), mRNA mm
MU19KSUBC:TC35077_AT Tubby like protein 4 (Tulp4), mRNA mm
RG-U34B:RC_AA963991_AT similar to tubby super-family protein rn
RAE230B:1382068_AT similar to tubby super-family protein rn
RAT230_2:1382068_AT similar to tubby super-family protein rn
Protein Similarities
Method ID Description E-Value
blast AAK31566 Temporarily assigned gene name protein 305 [Caenorhabditis elegans] ref|NP_490913.2| Temporarily Assigned Gene name family member (tag-305) [Caenorhabditis elegans] 0.0
blast CAE74471 Hypothetical protein CBG22218 [Caenorhabditis briggsae] 0.0
blast T15282 hypothetical protein M01D7.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000007 EMBL-EBI Tubby 1.2E-35
Pfam IPR000007 EMBL-EBI Tubby 1.2E-35
Sequence
Target Sequence 
>C. ELEGANS:176433_AT
gctggcgtttatcgacggggatgccgatgagaaggcgatgttggtgcagccggcgttgaa
gaccggatgggcagcgatggaacaagcgagtaaaaaggatgcggaggtgatacgatcgca
cgtggataagttggcatcaattgcagaacaattgagtaaacgacacggtgattttaatgt
gaaacgagacaaagcatcgatcaacaagatgcgctcacaaatgaagaccctcctgcggcg
agtcaacgagattgagcaaaaagtggcgaatggtgacgtgaaaaccgaagtccgtcagct
gctgaacacgttgaacgagatgaagaaggcgttgggtgagggtgtaccgaagagtggacg
aagtctcaattcaacgtgtaaaatcgagacaatgaccaataagacgccgttttggaacga
gcaaaatcaggtttatcagctggattttggaggccgcgtgactcaagagtcggccaagaa
tttccagatcgaattggacagcaaacaagttctgcaattcggcagaatcgaaggcggctc
gtacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGCGTTTATCGACGGGGATGCC 4 293 378 Antisense
TGTTGGTGCAGCCGGCGTTGAAGAC 590 563 416 Antisense
AAGACCGGATGGGCAGCGATGGAAC 481 153 436 Antisense
GCGGAGGTGATACGATCGCACGTGG 432 293 478 Antisense
TGCGCTCACAAATGAAGACCCTCCT 418 583 587 Antisense
TCCTGCGGCGAGTCAACGAGATTGA 645 593 608 Antisense
CGTGAAAACCGAAGTCCGTCAGCTG 546 245 654 Antisense
CGTCAGCTGCTGAACACGTTGAACG 79 245 670 Antisense
CAGCTGGATTTTGGAGGCCGCGTGA 327 203 814 Antisense
GAGTCGGCCAAGAATTTCCAGATCG 126 401 844 Antisense
GCAGAATCGAAGGCGGCTCGTACAC 145 311 899 Antisense
Sequence Source Affymetrix Proprietary Database