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[176429_at]

Description F54H12.2 /REP_DB=WormBase Gene ID /WP=CE00515 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 61      4grp_total > 10      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 4.9
(-3)
A 		54.5
(1.6)
A 		21.2
(-1.5)
A 		69
(3)
A 		64
B 5.3
(-2.7)
A 		6.9
(-2.3)
A 		3.7
(-3)
A 		31.7
(3)
A 		28
C 32.2
(-0.4)
A 		4.9
(-3)
A 		22.5
(-1.3)
A 		68.7
(3)
A 		64
avr 14.1
(-3) 		22.1
(-1.9) 		15.8
(-2.8) 		56.5
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 69.3
(3)
A 		21.2
(-1.6)
A 		48.1
(1)
A 		24.5
(-1.3)
A 		31.9
(-0.6)
A 		7.1
(-3)
A 		62
B 3
(-3)
A 		16.7
(-1)
A 		43.6
(3)
A 		20.4
(-0.4)
A 		22.4
(-0.1)
A 		20.4
(-0.4)
A 		41
C 5
(-3)
A 		12.2
(-2)
A 		4.8
(-3)
A 		47.6
(3)
A 		22.8
(-0.5)
A 		16.4
(-1.4)
A 		43
avr 25.8
(0.8) 		16.7
(-2.3) 		32.2
(3) 		30.8
(2.5) 		25.7
(0.8) 		14.6
(-3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.8
(-3)
A 		11.8
(3)
A 		5.6
(-0.7)
A 		10.1
(2)
A 		10
B 1.7
(-2.2)
A 		1.7
(-2.2)
A 		0.9
(-3)
A 		6.8
(3)
A 		6
C 10.7
(2.5)
A 		1.4
(-3)
A 		7.8
(0.8)
A 		11.6
(3)
A 		10
avr 4.7
(-3) 		5
(-2.6) 		4.8
(-2.9) 		9.5
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 12.8
(3)
A 		7.5
(0.1)
A 		10.5
(1.8)
A 		6.2
(-0.6)
A 		9.6
(1.3)
A 		1.7
(-3)
A 		11
B 1
(-3)
A 		5.6
(-0.1)
A 		10.6
(3)
A 		5.4
(-0.3)
A 		9.1
(2.1)
A 		7.6
(1.1)
A 		10
C 1.8
(-2.6)
A 		3.7
(-1.2)
A 		1.2
(-3)
A 		9.6
(3)
A 		7.4
(1.4)
A 		5.5
(0.1)
A 		8
avr 5.2
(-2.5) 		5.6
(-1.9) 		7.4
(0.9) 		7.1
(0.5) 		8.7
(3) 		4.9
(-3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176429_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6809
Sequence Type Exemplar sequence
Representative Public ID F54H12.2 NCBI
Target Description F54H12.2 /REP_DB=WormBase Gene ID /WP=CE00515 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066334(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066334 NCBI Caenorhabditis elegans F54H12.2 (F54H12.2) mRNA, complete cds. 11/11 A 
SNAP00000022459 ENSEMBL cdna:SNAP chromosome:CEL140:III:7962935:7965764:1 11/11 None
GENEFINDER00000022470 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7964505:7965764:1 11/11 None
F54H12.2 ENSEMBL cdna:known chromosome:CEL140:III:7964505:7965764:1 gene:F54H12.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006525 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006535 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7964502-7965762(+) 100.0 100.0
chrIV:14846415-14847052(+) 48.97 48.97
chrII:2272061-2273321(-) 93.33 93.33
Public Domain and Genome References
Gene Title
Ensembl F54H12.2
Entrez Gene 186266 Entrez gene
SwissProt P34456 EMBL-EBI
WormBase CE00515 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84536 Hypothetical protein F54H12.2 [Caenorhabditis elegans] ref|NP_498735.1| F54H12.2 [Caenorhabditis elegans] sp|P34456|YMD2_CAEEL Hypothetical protein F54H12.2 in chromosome III 0.0
blast AAB66195 Hypothetical protein K09F6.5 [Caenorhabditis elegans] ref|NP_494224.1| K09F6.5 [Caenorhabditis elegans] 0.0
blast AAG24065 Hypothetical protein F19G12.2 [Caenorhabditis elegans] ref|NP_508269.1| F19G12.2 [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:176429_AT
tcctctcaagttcgcagcttttacattgatggagggcggtttgacgctcctgcaaatact
ctatttacaagtaaaatgccgagaagactctttctgggtctagtttcaagcgaagcttat
aacggatcgttcggaacaacaccgttcaatttcaaaccgtacggagtaacagacgtccac
attgattattgtgggcaaacaatcccaggacgtccaatgaaccttgattttgaaaacaac
aaatttgtggaagcctacgttcaacttcaggaaacacttggtcacactagaaacaacttt
agctgcaattcgatcgacattggaatgtttagatccaaaggatatacgatttttggattc
gagttgagccctgtggctcaggataataatctgttcgaactagttcgtcaaacaaacgtt
agcgtacgcctcaatttcaaaaaggaaactccaccaggaggtctctattgtgtcgtct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCTCAAGTTCGCAGCTTTTACA 106 593 727 Antisense
TTGACGCTCCTGCAAATACTCTATT 468 703 767 Antisense
ATGCCGAGAAGACTCTTTCTGGGTC 668 41 802 Antisense
GGATCGTTCGGAACAACACCGTTCA 87 511 850 Antisense
GAGTAACAGACGTCCACATTGATTA 376 399 890 Antisense
GGGCAAACAATCCCAGGACGTCCAA 496 491 919 Antisense
GGACGTCCAATGAACCTTGATTTTG 79 525 934 Antisense
GTGGAAGCCTACGTTCAACTTCAGG 433 489 973 Antisense
ACTTCAGGAAACACTTGGTCACACT 285 101 990 Antisense
AACAACTTTAGCTGCAATTCGATCG 476 133 1018 Antisense
CCAGGAGGTCTCTATTGTGTCGTCT 119 273 1180 Antisense
Sequence Source Affymetrix Proprietary Database