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[176428_at]

Description Y71G12B.S /REP_DB=WormBase Gene ID /WP=CE22904 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 56      4grp_total > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 14
(0.3)
A 		14
(0.3)
A 		4.4
(-3)
A 		21.7
(3)
A 		17
B 20.3
(-0.4)
A 		4
(-3)
A 		4.8
(-2.9)
A 		42.2
(3)
A 		38
C 8
(-3)
A 		35.7
(1.4)
A 		29.7
(0.5)
A 		45.7
(3)
A 		38
avr 14.1
(-2.7) 		17.9
(-1.7) 		13
(-3) 		36.5
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 8.5
(-2.1)
A 		3.5
(-3)
A 		27.7
(1.5)
A 		34.6
(2.8)
A 		11.3
(-1.5)
A 		35.5
(3)
A 		32
B 11.2
(-2)
A 		36.5
(3)
A 		12
(-1.9)
A 		6.4
(-3)
A 		11.1
(-2.1)
A 		29.8
(1.7)
A 		30
C 18.9
(3)
A 		4.7
(-3)
A 		12.4
(0.3)
A 		4.7
(-3)
A 		5.5
(-2.7)
A 		4.7
(-3)
A 		14
avr 12.9
(-1.5) 		14.9
(-0.6) 		17.4
(0.5) 		15.2
(-0.5) 		9.3
(-3) 		23.3
(3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 5.1
(3)
A 		3
(-0.2)
A 		1.1
(-3)
A 		3.2
(0.2)
A 		4
B 6.5
(1.1)
A 		1
(-3)
A 		1.1
(-2.9)
A 		9
(3)
A 		8
C 2.7
(-3)
A 		10.3
(2.9)
A 		10.4
(3)
A 		7.7
(0.9)
A 		8
avr 4.8
(-1.5) 		4.8
(-1.5) 		4.2
(-3) 		6.6
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.6
(-2.7)
A 		1.2
(-3)
A 		6
(0.8)
A 		8.8
(3)
A 		3.4
(-1.3)
A 		8.6
(2.8)
A 		8
B 3.9
(-1.7)
A 		12.1
(3)
A 		2.9
(-2.3)
A 		1.7
(-3)
A 		4.5
(-1.4)
A 		11.1
(2.4)
A 		10
C 7
(3)
A 		1.4
(-2.5)
A 		3.2
(-0.7)
A 		0.9
(-3)
A 		1.8
(-2.1)
A 		1.6
(-2.3)
A 		6
avr 4.2
(-1.5) 		4.9
(-0.4) 		4
(-1.8) 		3.8
(-2.1) 		3.2
(-3) 		7.1
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176428_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18705
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.S NCBI
Target Description Y71G12B.S /REP_DB=WormBase Gene ID /WP=CE22904 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058480
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058480 NCBI Caenorhabditis elegans Y71G12B.16 (Y71G12B.16) mRNA, complete cds. None
GENEFINDER00000001825 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1678424:1685064:1 None
SNAP00000001781 ENSEMBL cdna:SNAP chromosome:CEL140:I:1682904:1685674:1 None
Y71G12B.16 ENSEMBL cdna:known chromosome:CEL140:I:1681814:1684572:1 gene:Y71G12B.16 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1681813-1686207(+) 88.12 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71G12B.16
Entrez Gene 190602 Entrez gene
SwissProt Q95XN8 EMBL-EBI
WormBase CE36760 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.13856.1.S1_AT RGM domain family, member B gga
MG-U74BV2:114342_AT RGM domain family, member B mm
MG-U74BV2:104997_AT RGM domain family, member B mm
MG-U74BV2:163507_AT RGM domain family, member B mm
MOE430B:1428430_AT RGM domain family, member B mm
MOUSE430_2:1428430_AT RGM domain family, member B mm
MOE430B:1428429_AT RGM domain family, member B mm
MOUSE430_2:1428429_AT RGM domain family, member B mm
MOE430B:1433587_AT RGM domain family, member B mm
MOUSE430_2:1433587_AT RGM domain family, member B mm
MOE430B:1433586_AT RGM domain family, member B mm
MOUSE430_2:1433586_AT RGM domain family, member B mm
MOE430B:1442811_AT RGM domain family, member B mm
MOUSE430_2:1442811_AT RGM domain family, member B mm
MOE430B:1459366_AT RGM domain family, member B mm
MOUSE430_2:1459366_AT RGM domain family, member B mm
MU19KSUBA:TC17755_AT RGM domain family, member B, mRNA (cDNA clone MGC:40725 IMAGE:5351870) mm
MU19KSUBB:TC31687_AT RGM domain family, member B, mRNA (cDNA clone MGC:40725 IMAGE:5351870) mm
Protein Similarities
Method ID Description E-Value
blast AAK73915 Hypothetical protein Y71G12B.16 [Caenorhabditis elegans] ref|NP_490881.2| Y71G12B.16 [Caenorhabditis elegans] 1.0E-177
blast CAE74415 Hypothetical protein CBG22147 [Caenorhabditis briggsae] 1.0E-161
blast AAK73915 Hypothetical protein Y71G12B.16 [Caenorhabditis elegans] ref|NP_490881.2| Y71G12B.16 [Caenorhabditis elegans] 1.0E-153
blast CAE74415 Hypothetical protein CBG22147 [Caenorhabditis briggsae] 1.0E-117
blast AAK73915 Hypothetical protein Y71G12B.16 [Caenorhabditis elegans] ref|NP_490881.2| Y71G12B.16 [Caenorhabditis elegans] 1.0E-103
blast CAE74415 Hypothetical protein CBG22147 [Caenorhabditis briggsae] 5.0E-89
blast NP_848730 repulsive guidance molecule B [Mus musculus] emb|CAD89719.1| repulsive guidance molecule B, RgmB protein [Mus musculus] sp|Q7TQ33|RGMB_MOUSE RGM domain family member B precursor 2.0E-27
blast AAT28341 dragon; Rgmb [Danio rerio] ref|XP_683312.1| PREDICTED: similar to dragon; Rgmb [Danio rerio] ref|NP_001001727.1| RGM domain family, member B [Danio rerio] 1.0E-22
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000001825 1 491-508
Sequence
Target Sequence 
>C. ELEGANS:176428_AT
gcgccgacgattgttctggaaactggaggagacgtggccagggagctttgttggtcggga
tgtaggaaaagctcacggatacctgcagagcttgctgttgagatgacgaaaaagtttgca
gagtgctacaggagacgggttcatgtgccgaaaaaggttgcggaagagactaccttctta
agtgagcaaaaagttttgccaatttacgatcgctgcaaagacattggcaacatcggagtg
ttcttcgacgcgagtgctagaaaaattttgaactttagagtttctgggagccaagttaca
tcccttcaaaattgcaaggcgcgtcgcggtctgcgtcgcggccaggctatcattttggag
cgctatttctcagctcccaaacccaaaaagtttcatctttgtacggcaactggaggacaa
gttacagcccttcaaagtttcgaggcgcgtcgcggtctgcgtcgcggtcaggctaccaca
gtggagcgctgtatctcggctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGCCGACGATTGTTCTGGAAACTG 692 285 646 Antisense
GACGTGGCCAGGGAGCTTTGTTGGT 319 375 676 Antisense
GGATACCTGCAGAGCTTGCTGTTGA 159 511 722 Antisense
AGTTTTGCCAATTTACGATCGCTGC 525 63 837 Antisense
GCAACATCGGAGTGTTCTTCGACGC 99 319 872 Antisense
GGGAGCCAAGTTACATCCCTTCAAA 459 495 931 Antisense
AAAATTGCAAGGCGCGTCGCGGTCT 500 133 953 Antisense
GTCGCGGCCAGGCTATCATTTTGGA 684 473 980 Antisense
TGGAGCGCTATTTCTCAGCTCCCAA 202 549 1001 Antisense
GCCCTTCAAAGTTTCGAGGCGCGTC 212 283 1072 Antisense
ACAGTGGAGCGCTGTATCTCGGCTC 482 107 1123 Antisense
Sequence Source Affymetrix Proprietary Database