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[176418_at]

Description F28A10.3 /REP_DB=WormBase Gene ID /WP=CE19408 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 48      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 48.4
(1.2)
A 		61.2
(2.6)
A 		64.5
(3)
A 		10.2
(-3)
A 		54
B 17.8
(-3)
A 		40.5
(-0.3)
A 		68
(3)
A 		34.7
(-1)
A 		50
C 40.6
(-1)
A 		46
(-0.2)
A 		28.1
(-3)
A 		66.5
(3)
A 		38
avr 35.6
(-3) 		49.2
(1.6) 		53.5
(3) 		37.1
(-2.5) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 95.2
(3)
A 		56.2
(-2)
A 		63.5
(-1)
A 		74.6
(0.4)
A 		69.6
(-0.3)
A 		48
(-3)
A 		47
B 45.1
(0.7)
A 		30
(-1.9)
A 		38.6
(-0.4)
A 		23.7
(-3)
A 		25.7
(-2.7)
A 		58.2
(3)
A 		35
C 30
(-2.3)
A 		37.1
(-1.5)
A 		74.3
(3)
A 		44.1
(-0.6)
A 		24.6
(-3)
A 		62.6
(1.6)
A 		50
avr 56.8
(2.4) 		41.1
(-2.6) 		58.8
(3) 		47.5
(-0.6) 		40
(-3) 		56.3
(2.2) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 17.6
(3)
A 		13.3
(1.4)
A 		16.9
(2.7)
A 		1.5
(-3)
A 		16
B 5.7
(-3)
A 		10
(-0.4)
A 		15.7
(3)
A 		7.4
(-2)
A 		10
C 13.4
(3)
A 		13.2
(2.7)
A 		9.8
(-3)
A 		11.2
(-0.7)
A 		4
avr 12.2
(1.5) 		12.2
(1.5) 		14.1
(3) 		6.7
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 17.6
(0.8)
A 		20
(2.4)
A 		13.8
(-1.6)
A 		18.9
(1.6)
A 		21
(3)
A 		11.7
(-3)
A 		9
B 15.7
(0.7)
A 		10
(-1.6)
A 		9.4
(-1.8)
A 		6.3
(-3)
A 		10.4
(-1.4)
A 		21.7
(3)
A 		15
C 11.1
(-1.6)
A 		11.3
(-1.5)
A 		18.9
(2)
A 		8.9
(-2.6)
A 		8
(-3)
A 		21
(3)
A 		13
avr 14.8
(0) 		13.8
(-0.9) 		14
(-0.7) 		11.4
(-3) 		13.1
(-1.5) 		18.1
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176418_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5329
Sequence Type Exemplar sequence
Representative Public ID F28A10.3 NCBI
Target Description F28A10.3 /REP_DB=WormBase Gene ID /WP=CE19408 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061428(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061428 NCBI Caenorhabditis elegans F28A10.3 (F28A10.3) mRNA, complete cds. 11/11 None
F28A10.3 ENSEMBL cdna:known chromosome:CEL140:II:840573:841119:1 gene:F28A10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:840571-841119(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28A10.3
Entrez Gene 185029 Entrez gene
SwissProt Q95YB5 EMBL-EBI
WormBase CE29780 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85469 Hypothetical protein F28A10.3 [Caenorhabditis elegans] ref|NP_493829.2| F28A10.3 [Caenorhabditis elegans] 2.0E-53
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493829.2 3 5-27,34-56,83-105
F28A10.3 3 5-27,34-56,83-105
Sequence
Target Sequence 
>C. ELEGANS:176418_AT
gacattggtggcacctactccattcttgctttcatctttgaccaccaagtcaggcttctc
gtttttgactcgcattctttttttcgtcattgctggattctctgcactcacctgcctttt
catggttactgccgttactttggtgggattagatgtgaaaaaatttaatgatgataacgc
aaaattcaacaacataagtattttaaagttgggctggtctgcttggatgtgtgttgcgtc
tgctgtgttgatggctggaagtgctgcacttgcggttctcattggtctttttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACATTGGTGGCACCTACTCCATTC 108 369 15 Antisense
CTTGCTTTCATCTTTGACCACCAAG 418 219 39 Antisense
AAGTCAGGCTTCTCGTTTTTGACTC 456 157 61 Antisense
GACTCGCATTCTTTTTTTCGTCATT 167 375 81 Antisense
TCATTGCTGGATTCTCTGCACTCAC 362 619 101 Antisense
TGGTTACTGCCGTTACTTTGGTGGG 544 547 137 Antisense
TAAAGTTGGGCTGGTCTGCTTGGAT 236 641 218 Antisense
TCTGCTTGGATGTGTGTTGCGTCTG 55 609 232 Antisense
TTGCGTCTGCTGTGTTGATGGCTGG 686 701 248 Antisense
GTTGATGGCTGGAAGTGCTGCACTT 155 439 261 Antisense
TGCGGTTCTCATTGGTCTTTTTGGA 237 583 285 Antisense
Sequence Source Affymetrix Proprietary Database