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[176415_at]

Description Y71G12B.Z /REP_DB=WormBase Gene ID /WP=CE26263 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 67      4grp_highest_8_8 > 33      4grp_high_8_8 > 51      6grp_highest_nonorm > 92      6grp_total > 67      4grp_total > 61      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 357
1 2 3 4 range
A 545.5
(3)
P 		507.4
(2.2)
P 		515.8
(2.4)
P 		243.5
(-3)
P 		302
B 600.1
(3)
P 		477.1
(0.7)
P 		514.6
(1.4)
P 		277.4
(-3)
P 		323
C 545.1
(2.8)
P 		544.7
(2.8)
P 		556.5
(3)
P 		264.7
(-3)
P 		292
avr 563.6
(3) 		509.7
(1.9) 		529
(2.3) 		261.9
(-3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 205.2
(-2.6)
P 		232.6
(-1.8)
P 		192.7
(-3)
P 		218.2
(-2.2)
P 		270.7
(-0.6)
P 		391.3
(3)
P 		199
B 262.7
(-2.1)
P 		235.6
(-3)
P 		259.9
(-2.2)
P 		253.8
(-2.4)
P 		279.7
(-1.6)
P 		420.4
(3)
P 		185
C 268
(-1.3)
P 		355.4
(1.5)
P 		255
(-1.8)
P 		217.6
(-3)
P 		224
(-2.8)
P 		399.7
(3)
P 		182
avr 245.3
(-2.5) 		274.5
(-1.5) 		235.9
(-2.8) 		229.9
(-3) 		258.1
(-2) 		403.8
(3) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 163
1 2 3 4 range
A 198.6
(3)
P 		110.2
(-0.3)
P 		134.8
(0.7)
P 		35.6
(-3)
P 		163
B 191.4
(3)
P 		117.8
(-0.3)
P 		119.3
(-0.3)
P 		59.1
(-3)
P 		132
C 180.5
(2.5)
P 		156.6
(1.5)
P 		194
(3)
P 		44.6
(-3)
P 		149
avr 190.2
(3) 		128.2
(0.4) 		149.4
(1.3) 		46.4
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 37.8
(-3)
P 		82.7
(1.7)
P 		42
(-2.6)
P 		55.3
(-1.2)
P 		81.7
(1.6)
P 		95.3
(3)
P 		58
B 91.4
(-1.2)
P 		78.4
(-2)
P 		63.1
(-3)
P 		67.2
(-2.7)
P 		113.6
(0.2)
P 		156.8
(3)
P 		94
C 99.3
(0.7)
P 		108.5
(1.3)
P 		64.9
(-1.6)
P 		43.8
(-3)
P 		72.5
(-1.1)
P 		133.8
(3)
P 		90
avr 76.2
(-1.3) 		89.9
(-0.2) 		56.7
(-2.9) 		55.4
(-3) 		89.3
(-0.2) 		128.6
(3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176415_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18710
Sequence Type Exemplar sequence
Representative Public ID Y71G12B.Z NCBI
Target Description Y71G12B.Z /REP_DB=WormBase Gene ID /WP=CE26263 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058490(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058490 NCBI Caenorhabditis elegans Y71G12B.8 (Y71G12B.8) mRNA, complete cds. 11/11 A 
SNAP00000001793 ENSEMBL cdna:SNAP chromosome:CEL140:I:1782753:1790006:1 11/11 None
GENEFINDER00000001828 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1783693:1790006:1 11/11 None
Y71G12B.8 ENSEMBL cdna:known chromosome:CEL140:I:1767888:1790156:1 gene:Y71G12B.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1781991-1787488(+) 92.33 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71G12B.8
Entrez Gene 171743 Entrez gene
SwissProt Q95XM9 EMBL-EBI
WormBase CE27030 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:19203_S_AT DEAD/DEAH box helicase, putative (RH28) at
ATH1-121501:245437_AT DEAD/DEAH box helicase, putative (RH28) at
CANINE:1591729_AT similar to Probable ATP-dependent RNA helicase DDX27 (DEAD-box protein 27) cfa
CANINE:1591701_AT similar to Probable ATP-dependent RNA helicase DDX27 (DEAD-box protein 27) cfa
CANINE_2:CFA.16395.1.S1_S_AT similar to Probable ATP-dependent RNA helicase DDX27 (DEAD-box protein 27) cfa
CANINE_2:CFAAFFX.17734.1.S1_AT similar to Probable ATP-dependent RNA helicase DDX27 (DEAD-box protein 27) cfa
DROSOPHILA_2:1631198_AT dm
DROSGENOME1:153844_AT dm
CHICKEN:GGA.5530.1.S1_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 gga
CHICKEN:GGAAFFX.11907.1.S1_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 gga
HG-U133_PLUS_2:215693_X_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133A_2:215693_X_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133A:215693_X_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133_PLUS_2:219108_X_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133A:219108_X_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133A_2:219108_X_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HU35KSUBC:RC_T15477_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
U133_X3P:HS.65234.1.S1_3P_A_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133A_2:221780_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133_PLUS_2:221780_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U133A:221780_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U95AV2:33650_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
HG-U95B:46595_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 hs
MOE430A:1425541_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MOUSE430A_2:1425541_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MOUSE430_2:1425541_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MOUSE430_2:1424161_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MOE430A:1424161_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MOUSE430A_2:1424161_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MU11KSUBA:AA210605_F_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MU11KSUBB:MSA.10198.0_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MG-U74BV2:107631_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MG-U74BV2:109001_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MG-U74AV2:95540_R_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 mm
MU19KSUBA:TC14941_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA mm
MU19KSUBA:TC24377_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA mm
MU19KSUBC:TC39456_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA mm
MU19KSUBC:TC41966_F_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA mm
MU19KSUBC:TC41966_I_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA mm
MU19KSUBC:TC41967_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA mm
RAE230B:1397551_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (predicted) rn
RAT230_2:1397551_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (predicted) rn
RAE230B:1385471_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (predicted) rn
RAT230_2:1385471_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (predicted) rn
YEAST_2:1773948_AT Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK73934 Hypothetical protein Y71G12B.8 [Caenorhabditis elegans] ref|NP_490891.1| Y71G12B.8 [Caenorhabditis elegans] 0.0
blast CAE74433 Hypothetical protein CBG22166 [Caenorhabditis briggsae] 0.0
blast AAH97561 Unknown (protein for MGC:114699) [Xenopus laevis] 1.0E-143
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.4E-32
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 7.9E-55
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 9.6E-25
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.4E-32
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 8.2E-41
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.4E-32
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.1E-40
Sequence
Target Sequence 
>C. ELEGANS:176415_AT
aatgcagtcgagtcttacacagggacaacgtatcgaatcgctgtcaaagttcaaaaaagc
tgaaattgatgtgctggtgtctactgatttggcatctcgtggtcttgatattgaaggtgt
tcaaacagtaatcaacatgaatatgccaaaatcaatcaaacaatacattcatcgtgtcgg
acgtactgcaagagccggaaaagcgggaagatcaatctcacttgtgggagaagacgaacg
aaaattgttgaaagaaattgtgaattcgaacgccgatcgaacactgaaacaacgtcttgt
tgcaccggaagtcgttgaagcgtatagaagacggatcgatgagcttgaagagactattca
acagattgatgaagaggatcgtgctgaaaaagagcttcgtattgctgaagcttcaatggc
gaagacacaaaatgcactggaagttggagagaatggaggtgctccggctgagagaagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCAGTCGAGTCTTACACAGGGA 333 165 684 Antisense
CAGGGACAACGTATCGAATCGCTGT 154 205 703 Antisense
GAAATTGATGTGCTGGTGTCTACTG 217 363 745 Antisense
GGTGTCTACTGATTTGGCATCTCGT 133 501 759 Antisense
GGCATCTCGTGGTCTTGATATTGAA 568 535 774 Antisense
TCGTGTCGGACGTACTGCAAGAGCC 307 605 855 Antisense
GCGGGAAGATCAATCTCACTTGTGG 301 293 886 Antisense
GCCGATCGAACACTGAAACAACGTC 469 283 955 Antisense
GAAACAACGTCTTGTTGCACCGGAA 78 357 969 Antisense
GTTGCACCGGAAGTCGTTGAAGCGT 120 439 982 Antisense
GGAGGTGCTCCGGCTGAGAGAAGAG 196 517 1138 Antisense
Sequence Source Affymetrix Proprietary Database