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[176414_s_at]

Description C30F12.2 /REP_DB=WormBase Gene ID /WP=CE16882 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 76      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_total > 76      4grp_total > 22      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 128
(0.7)
P 		100
(-3)
P 		115.7
(-0.9)
P 		145.7
(3)
P 		46
B 123.3
(3)
P 		114
(-1.2)
P 		110
(-3)
P 		118.8
(1)
P 		13
C 124.2
(0.6)
P 		133.6
(3)
P 		123.5
(0.4)
P 		110.5
(-3)
P 		23
avr 125.2
(3) 		115.9
(-3) 		116.4
(-2.7) 		125
(2.9) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 84.4
(-3)
M 		151.1
(0.4)
P 		202.1
(3)
P 		147.5
(0.2)
P 		154.3
(0.6)
P 		141
(-0.1)
P 		118
B 160.3
(-2.6)
P 		154.9
(-3)
P 		169.7
(-2)
P 		166.8
(-2.2)
P 		174.6
(-1.6)
P 		242
(3)
P 		87
C 163.1
(-0.5)
P 		184.2
(1.9)
P 		151
(-1.9)
P 		141.8
(-3)
P 		187.5
(2.3)
P 		193.6
(3)
P 		52
avr 135.9
(-3) 		163.4
(-0.1) 		174.3
(1.1) 		152
(-1.3) 		172.1
(0.9) 		192.2
(3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 46.6
(3)
P 		21.7
(-2.9)
P 		30.2
(-0.9)
P 		21.3
(-3)
P 		25
B 39.3
(3)
P 		28.2
(-1.8)
P 		25.5
(-2.9)
P 		25.3
(-3)
P 		14
C 41.1
(2.5)
P 		38.4
(1.9)
P 		43
(3)
P 		18.6
(-3)
P 		24
avr 42.3
(3) 		29.4
(-0.8) 		32.9
(0.3) 		21.7
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 15.6
(-3)
M 		53.7
(3)
P 		44
(1.5)
P 		37.4
(0.4)
P 		46.5
(1.9)
P 		34.3
(-0.1)
P 		38
B 55.8
(-1.2)
P 		51.6
(-1.7)
P 		41.2
(-3)
P 		44.2
(-2.6)
P 		70.9
(0.6)
P 		90.2
(3)
P 		49
C 60.4
(2.3)
P 		56.2
(1.6)
P 		38.4
(-1.4)
P 		28.5
(-3)
P 		60.7
(2.3)
P 		64.8
(3)
P 		36
avr 43.9
(-1.4) 		53.8
(0.9) 		41.2
(-2) 		36.7
(-3) 		59.4
(2.2) 		63.1
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176414_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.852
Sequence Type Exemplar sequence
Representative Public ID C30F12.2 NCBI
Target Description C30F12.2 /REP_DB=WormBase Gene ID /WP=CE16882 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059585(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059585 NCBI Caenorhabditis elegans C30F12.2 (C30F12.2) mRNA, complete cds. 11/11 None
SNAP00000017404 ENSEMBL cdna:SNAP chromosome:CEL140:I:6976054:6977692:-1 11/11 None
GENEFINDER00000017418 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6976054:6978272:-1 11/11 None
C30F12.2.1 ENSEMBL cdna:known chromosome:CEL140:I:6975813:6978277:-1 gene:C30F12.2 11/11 None
C30F12.2.2 ENSEMBL cdna:known chromosome:CEL140:I:6975813:6978322:-1 gene:C30F12.2 11/11 None
C30F12.2.3 ENSEMBL cdna:known chromosome:CEL140:I:6976054:6978279:-1 gene:C30F12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6975984-6978203(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C30F12.2
Entrez Gene 172427 Entrez gene
SwissProt Q95YE1 EMBL-EBI
WormBase CE16882 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:253630_AT AFG1-like ATPase family protein at
CANINE_2:CFAAFFX.6603.1.S1_AT similar to lactation elevated 1 cfa
DROSOPHILA_2:1624157_AT dm
DROSGENOME1:154207_AT dm
CHICKEN:GGAAFFX.9753.1.S1_AT similar to lactation elevated 1; CG8520 gene product; lactation elevated-1 gga
CHICKEN:GGAAFFX.9753.2.S1_AT similar to lactation elevated 1; CG8520 gene product; lactation elevated-1 gga
CHICKEN:GGAAFFX.9753.2.S1_S_AT similar to lactation elevated 1; CG8520 gene product; lactation elevated-1 gga
U133_X3P:HS2.376951.1.S1_3P_S_AT lactation elevated 1 hs
U133_X3P:HS2.376951.1.S1_3P_AT lactation elevated 1 hs
U133_X3P:HS2.164285.1.S1_3P_AT lactation elevated 1 hs
U133_X3P:1563671_3P_AT lactation elevated 1 hs
HG-U133_PLUS_2:1552490_AT lactation elevated 1 hs
HG-U133_PLUS_2:1563671_AT lactation elevated 1 hs
HG-U95D:84685_AT lactation elevated 1 hs
MG-U74CV2:135381_I_AT lactation elevated 1 mm
MG-U74CV2:135382_R_AT lactation elevated 1 mm
MOE430A:1427073_AT lactation elevated 1 mm
MOUSE430A_2:1427073_AT lactation elevated 1 mm
MOUSE430_2:1427073_AT lactation elevated 1 mm
MG-U74CV2:133454_AT Lactation elevated 1 (Lace1), mRNA mm
MU19KSUBC:TC38570_AT Lactation elevated 1 (Lace1), mRNA mm
YEAST_2:1772946_AT Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family, localized to mitochondria Sc
Protein Similarities
Method ID Description E-Value
blast AAK85448 Hypothetical protein C30F12.2 [Caenorhabditis elegans] ref|NP_491986.1| C30F12.2 [Caenorhabditis elegans] 0.0
blast CAE60342 Hypothetical protein CBG03934 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005654 EMBL-EBI AFG1-like ATPase 2.0E-66
Pfam IPR005654 EMBL-EBI AFG1-like ATPase 2.0E-4
Pfam IPR005654 EMBL-EBI AFG1-like ATPase 3.6E-93
Pfam IPR005654 EMBL-EBI AFG1-like ATPase 2.0E-4
Sequence
Target Sequence 
>C. ELEGANS:176414_S_AT
ttaccgtcgcagtgcagctggagacggaaatcatgtttatttcagttcagaagattctaa
tacacaatgtgacatagttttcaaacaatctgcagctaatgagaatgatactgtacgttc
gaaaactctcgaaatcttgggacgtcgagtgatcgtcgagaaatgttgtggtggagttgc
tgatgtggatttcaaagagctctgcatgacagcgaagggagcagttgattatcttgttta
tgcacgagtatttcatacagttattgtaagaaatattccaattatgaaccaagatatgtg
gaatgctatgagacgattcatcacaatgattgacacattttatgatcagaaggtccgtgt
tgtaattggagcagctgctcctttagatgagctctttcaatttgaaggacacaatacttc
tcacgacgctctaagtgactcaaaacgaatgctcatggatgatctgggtatcaaatctga
tcatgaaggaatgagcgcaaatgtgttcagtggagatgaagaagcattcgcctacagtag
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTACCGTCGCAGTGCAGCTGGAGAC 270 683 726 Antisense
GAAAACTCTCGAAATCTTGGGACGT 301 355 846 Antisense
GTTGTGGTGGAGTTGCTGATGTGGA 189 437 890 Antisense
GATGTGGATTTCAAAGAGCTCTGCA 302 409 907 Antisense
GAGCTCTGCATGACAGCGAAGGGAG 10 389 922 Antisense
GATTATCTTGTTTATGCACGAGTAT 632 429 952 Antisense
CGTGTTGTAATTGGAGCAGCTGCTC 489 245 1081 Antisense
GCAGCTGCTCCTTTAGATGAGCTCT 342 313 1096 Antisense
GAAGGACACAATACTTCTCACGACG 107 335 1129 Antisense
TTCTCACGACGCTCTAAGTGACTCA 187 689 1143 Antisense
GAAGAAGCATTCGCCTACAGTAGGA 324 339 1243 Antisense
Sequence Source Affymetrix Proprietary Database