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[176410_at]

Description C30F12.5 /REP_DB=WormBase Gene ID /WP=CE16885 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 0      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 56      6grp_total > 03      4grp_total > 59      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1772
1 2 3 4 range
A 1526.9
(0.3)
P 		2318.6
(3)
P 		1910.8
(1.6)
P 		551.7
(-3)
P 		1767
B 1764.4
(2.4)
P 		1900.6
(3)
P 		1805.5
(2.6)
P 		546.8
(-3)
P 		1354
C 1540.2
(1.5)
P 		1634.9
(2)
P 		1837.6
(3)
P 		624.6
(-3)
P 		1213
avr 1610.5
(1.5) 		1951.4
(3) 		1851.3
(2.6) 		574.4
(-3) 		1377

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1840
1 2 3 4 5 6 range
A 2722.9
(3)
P 		2090.6
(0.9)
P 		1637.8
(-0.6)
P 		2578.7
(2.5)
P 		1998.2
(0.6)
P 		928.1
(-3)
P 		1795
B 2087.6
(2.4)
P 		1736.2
(0.8)
P 		1926.6
(1.7)
P 		2227.3
(3)
P 		1928.5
(1.7)
P 		883
(-3)
P 		1344
C 1982.9
(1.2)
P 		1868.8
(0.7)
P 		2279.6
(2.4)
P 		2445
(3)
P 		2040.3
(1.4)
P 		917
(-3)
P 		1528
avr 2264.5
(2.4) 		1898.5
(0.9) 		1948
(1.1) 		2417
(3) 		1989
(1.3) 		909.4
(-3) 		1508
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 560
1 2 3 4 range
A 556
(3)
P 		503.3
(2.3)
P 		499.5
(2.3)
P 		80.8
(-3)
P 		475
B 562.6
(3)
P 		469.4
(1.7)
P 		418.4
(1.1)
P 		116.5
(-3)
P 		446
C 509.9
(1.5)
P 		470.1
(1.1)
P 		640.5
(3)
P 		105.3
(-3)
P 		535
avr 542.8
(3) 		480.9
(2.2) 		519.5
(2.7) 		100.9
(-3) 		442

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 558
1 2 3 4 5 6 range
A 501.9
(0.2)
P 		743.5
(3)
P 		356.7
(-1.5)
P 		654
(2)
P 		603
(1.4)
P 		226
(-3)
P 		518
B 726.4
(2.2)
P 		577.9
(0.3)
P 		467.8
(-1.2)
P 		589.9
(0.4)
P 		783.5
(3)
P 		329.3
(-3)
P 		454
C 735
(3)
P 		570.2
(0.7)
P 		580.2
(0.8)
P 		491.7
(-0.4)
P 		660.2
(2)
P 		307
(-3)
P 		428
avr 654.4
(2.6) 		630.5
(2.2) 		468.2
(-0.3) 		578.5
(1.4) 		682.2
(3) 		287.4
(-3) 		395
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176410_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1376
Sequence Type Exemplar sequence
Representative Public ID C30F12.5 NCBI
Target Description C30F12.5 /REP_DB=WormBase Gene ID /WP=CE16885 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059590(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059590 NCBI Caenorhabditis elegans C30F12.5 (C30F12.5) mRNA, complete cds. 11/11 None
SNAP00000017409 ENSEMBL cdna:SNAP chromosome:CEL140:I:7000444:7002698:-1 11/11 None
GENEFINDER00000017417 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7000444:7005825:-1 11/11 None
C30F12.5 ENSEMBL cdna:known chromosome:CEL140:I:7000360:7007703:-1 gene:C30F12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7000290-7007586(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C30F12.5
Entrez Gene 183055 Entrez gene
SwissProt Q95YD6 EMBL-EBI
WormBase CE16885 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85451 Hypothetical protein C30F12.5 [Caenorhabditis elegans] ref|NP_491991.1| C30F12.5 [Caenorhabditis elegans] 1.0E-176
blast CAE60347 Hypothetical protein CBG03940 [Caenorhabditis briggsae] 1.0E-156
blast AAK85451 Hypothetical protein C30F12.5 [Caenorhabditis elegans] ref|NP_491991.1| C30F12.5 [Caenorhabditis elegans] 1.0E-117
blast CAE60347 Hypothetical protein CBG03940 [Caenorhabditis briggsae] 1.0E-114
blast AAK85451 Hypothetical protein C30F12.5 [Caenorhabditis elegans] ref|NP_491991.1| C30F12.5 [Caenorhabditis elegans] 4.0E-94
blast CAE60347 Hypothetical protein CBG03940 [Caenorhabditis briggsae] 6.0E-93
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491991.1 1 198-220
C30F12.5 1 198-220
SNAP00000017409 1 85-107
GENEFINDER00000017417 2 83-105,248-270
Sequence
Target Sequence 
>C. ELEGANS:176410_AT
gtatgcatcgtggcttccagtgattggacttattgttgctgtgctccttgtgctttgtct
tgtttcaattcttctctcaatgatctgtgccaaatgtttctgctgttgtcgtaaaaataa
gaaagaaaagtacgaagttacgaaaccagcaacttacaagtctattgagcctctctacgt
tatcactccaccttccacgttgaatccacgtcaccacgatgcaatctactcaaccccata
ctctggaagtcctctaccatacccaccaccaccaggaacaaccgtaccacatactccaaa
ctcaacatacagatatcgagttgacacaaatagaaaaggacagaaagattatgaaaatgg
ttcaatgggacaactaccaattgatgaaccgagaataggacatcctctatattcaccact
tccaccaattgacccgacttatggaactattccacgtgctcgtcttgctccgaatggagc
acctccacctccattatctccatcatattcatctggaaatgacccacacttcttggatcc
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATGCATCGTGGCTTCCAGTGATT 459 455 585 Antisense
GTCTTGTTTCAATTCTTCTCTCAAT 187 469 641 Antisense
GATCTGTGCCAAATGTTTCTGCTGT 254 419 666 Antisense
GTCTATTGAGCCTCTCTACGTTATC 288 467 744 Antisense
TTGAATCCACGTCACCACGATGCAA 294 703 784 Antisense
TCAACCCCATACTCTGGAAGTCCTC 379 631 814 Antisense
TACCACATACTCCAAACTCAACATA 19 651 869 Antisense
ATAGGACATCCTCTATATTCACCAC 541 21 979 Antisense
TTCCACCAATTGACCCGACTTATGG 708 687 1004 Antisense
CCTCCATTATCTCCATCATATTCAT 172 267 1072 Antisense
AAATGACCCACACTTCTTGGATCCA 53 119 1101 Antisense
Sequence Source Affymetrix Proprietary Database