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[176402_at]

Description Y57E12B.B /REP_DB=WormBase Gene ID /WP=CE26551 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 42      4grp_total > 50      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 4.1
(-3)
A 		91.4
(3)
A 		61
(0.9)
A 		40.1
(-0.5)
A 		87
B 4.5
(-3)
A 		120.4
(3)
M 		65.4
(0.2)
A 		34.3
(-1.5)
A 		116
C 21.1
(-1.8)
A 		79.8
(2.5)
A 		86
(3)
P 		5.3
(-3)
A 		81
avr 9.9
(-3) 		97.2
(3) 		70.8
(1.2) 		26.6
(-1.9) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 115.2
(2)
P 		61.7
(-1)
P 		133.7
(3)
P 		56
(-1.3)
A 		25.2
(-3)
A 		28.9
(-2.8)
A 		109
B 93.3
(1.9)
P 		75
(0.9)
A 		102.9
(2.5)
P 		111.6
(3)
P 		52.5
(-0.4)
P 		8.7
(-3)
A 		103
C 97.6
(1.5)
P 		54.5
(-1.8)
A 		116.1
(3)
P 		115.3
(2.9)
P 		45.9
(-2.5)
P 		39.7
(-3)
A 		76
avr 102
(2) 		63.7
(-0.5) 		117.6
(3) 		94.3
(1.5) 		41.2
(-2) 		25.8
(-3) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 1.5
(-3)
A 		19.8
(3)
A 		15.9
(1.7)
A 		5.9
(-1.6)
A 		18
B 1.4
(-3)
A 		29.7
(3)
M 		15.2
(-0.1)
A 		7.3
(-1.7)
A 		28
C 7
(-1.7)
A 		22.9
(1.5)
A 		30
(3)
P 		0.9
(-3)
A 		29
avr 3.3
(-3) 		24.1
(3) 		20.4
(1.9) 		4.7
(-2.6) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 21.2
(0.9)
P 		22
(1.1)
P 		29.1
(3)
P 		14.2
(-1)
A 		7.6
(-2.8)
A 		7
(-3)
A 		22
B 32.5
(3)
P 		25
(1.5)
A 		25
(1.5)
P 		29.6
(2.4)
P 		21.3
(0.7)
P 		3.2
(-3)
A 		29
C 36.2
(3)
P 		16.6
(-2.1)
A 		29.6
(1.3)
P 		23.2
(-0.4)
P 		14.9
(-2.6)
P 		13.3
(-3)
A 		23
avr 30
(3) 		21.2
(0.6) 		27.9
(2.4) 		22.3
(0.9) 		14.6
(-1.2) 		7.8
(-3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176402_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19415
Sequence Type Exemplar sequence
Representative Public ID Y57E12B.B NCBI
Target Description Y57E12B.B /REP_DB=WormBase Gene ID /WP=CE26551 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072354(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072354 NCBI Caenorhabditis elegans Y57E12B.3 (Y57E12B.3) mRNA, complete cds. 11/11 None
SNAP00000007821 ENSEMBL cdna:SNAP chromosome:CEL140:V:6934215:6936899:-1 11/11 None
GENEFINDER00000007827 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6934215:6938454:-1 11/11 None
Y57E12B.3 ENSEMBL cdna:known chromosome:CEL140:V:6934143:6938454:-1 gene:Y57E12B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6934143-6938455(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y57E12B.3
Entrez Gene 179081 Entrez gene
SwissProt Q95XV1 EMBL-EBI
WormBase CE26551 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15913_AT lipase family protein at
ATH1-121501:263359_AT lipase family protein at
CANINE_2:CFAAFFX.24020.1.S1_AT similar to lipase-like, ab-hydrolase domain containing 2 cfa
DROSOPHILA_2:1632393_AT dm
DROSGENOME1:146166_AT dm
MOE430B:1443914_AT Lipase-like, ab-hydrolase domain containing 2, mRNA (cDNA clone MGC:67675 IMAGE:4011597) mm
MOUSE430_2:1443914_AT Lipase-like, ab-hydrolase domain containing 2, mRNA (cDNA clone MGC:67675 IMAGE:4011597) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16789 carboxylic ester hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68537 Hypothetical protein Y57E12B.3 [Caenorhabditis elegans] ref|NP_504755.1| Y57E12B.3 [Caenorhabditis elegans] 0.0
blast CAE71971 Hypothetical protein CBG19044 [Caenorhabditis briggsae] 0.0
blast CAB01973 Hypothetical protein F54F3.3 [Caenorhabditis elegans] ref|NP_506229.1| F54F3.3 [Caenorhabditis elegans] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 4.7E-12
Pfam IPR006693 EMBL-EBI Ab-hydrolase associated lipase region 1.2E-36
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 4.7E-12
Pfam IPR006693 EMBL-EBI Ab-hydrolase associated lipase region 1.2E-36
Pfam IPR000073 EMBL-EBI Alpha/beta hydrolase fold 4.7E-12
Pfam IPR006693 EMBL-EBI Ab-hydrolase associated lipase region 1.2E-36
Sequence
Target Sequence 
>C. ELEGANS:176402_AT
ggcgagttcctgaccaacaatattttcactaaactattaactgatattttttgcgaccaa
gcggtgaataatccactttgcgagaattttatctttgccgtttctggaccaaacagtaat
caattcaataactcgagaattggaatatacttagcacacaatccagctggcacgtcttct
agaaatattcttcattttgcacaaatggtcaagaaaaagcgtatgtctagattcgatcat
ggaaaagatttgaatttgaaaatttacggagctccttctcccccagaatatgatattcgt
aaaatcaacagctcaatttatttattctattctgattttgactggttagcaaatccaaaa
gatgtcgaaggatttttaattccaatgctgccatcgaaaactctcaaaaaagccacaaaa
cttcgagatttcaatcacaacgattttctatggggaatgagagcaagaaaggaaatttat
gacaaaataataaacaccatcaaattggatcaaagaagagtgaagttgcaaaatagtatg
gagagattctttgaaaggcaatcacgaaattcaacttccggtctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCGAGTTCCTGACCAACAATATTT 478 541 1438 Antisense
GATATTTTTTGCGACCAAGCGGTGA 514 425 1480 Antisense
AGCGGTGAATAATCCACTTTGCGAG 580 81 1497 Antisense
TTTTATCTTTGCCGTTTCTGGACCA 215 675 1524 Antisense
CTTAGCACACAATCCAGCTGGCACG 444 221 1587 Antisense
AGCTGGCACGTCTTCTAGAAATATT 328 81 1602 Antisense
AAAAGCGTATGTCTAGATTCGATCA 471 131 1652 Antisense
TGAAAATTTACGGAGCTCCTTCTCC 321 573 1694 Antisense
TATTCTATTCTGATTTTGACTGGTT 155 665 1760 Antisense
AATTCCAATGCTGCCATCGAAAACT 680 175 1815 Antisense
ATCACGAAATTCAACTTCCGGTCTT 28 25 1998 Antisense
Sequence Source Affymetrix Proprietary Database