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[176400_at]

Description Y57E12B.A /REP_DB=WormBase Gene ID /WP=CE26550 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 93      4grp_total > 11      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 24
(-2.1)
A 		11.8
(-2.8)
A 		7.4
(-3)
A 		122
(3)
A 		115
B 45.3
(-0.8)
A 		63.6
(0.2)
A 		5.9
(-3)
A 		113.4
(3)
M 		108
C 40.3
(-3)
A 		52.3
(-2.5)
A 		44.6
(-2.8)
A 		174.8
(3)
P 		135
avr 36.5
(-2.1) 		42.6
(-1.8) 		19.3
(-3) 		136.7
(3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1938
1 2 3 4 5 6 range
A 35.6
(-2.9)
A 		4.9
(-3)
A 		38.1
(-2.9)
A 		724.4
(-0.8)
P 		1942.6
(3)
P 		351.5
(-1.9)
P 		1938
B 23.6
(-3)
A 		58.5
(-2.9)
A 		42.7
(-2.9)
A 		734.5
(-0.8)
P 		1920.5
(3)
P 		261.7
(-2.2)
P 		1897
C 46.3
(-3)
A 		78.2
(-2.8)
A 		35.2
(-3)
A 		897
(0.1)
P 		1691.1
(3)
P 		330.4
(-1.9)
P 		1656
avr 35.2
(-3) 		47.2
(-3) 		38.7
(-3) 		785.3
(-0.5) 		1851.4
(3) 		314.5
(-2.1) 		1816
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 8.7
(-0.5)
A 		2.6
(-2.7)
A 		1.9
(-3)
A 		17.9
(3)
A 		16
B 14.5
(0.4)
A 		15.7
(0.8)
A 		1.4
(-3)
A 		24.2
(3)
M 		23
C 13.4
(-3)
A 		15
(-2.4)
A 		15.6
(-2.2)
A 		29.5
(3)
P 		16
avr 12.2
(-1) 		11.1
(-1.4) 		6.3
(-3) 		23.9
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 779
1 2 3 4 5 6 range
A 6.6
(-3)
A 		1.8
(-3)
A 		8.3
(-2.9)
A 		183.7
(-1.1)
P 		586.2
(3)
P 		85.6
(-2.1)
P 		584
B 8.2
(-3)
A 		19.5
(-2.9)
A 		10.4
(-3)
A 		194.5
(-1.6)
P 		780.3
(3)
P 		97.6
(-2.3)
P 		772
C 17.2
(-2.9)
A 		23.9
(-2.8)
A 		9
(-3)
A 		180.4
(-1.1)
P 		547.2
(3)
P 		110.6
(-1.9)
P 		538
avr 10.7
(-3) 		15.1
(-2.9) 		9.2
(-3) 		186.2
(-1.3) 		637.9
(3) 		97.9
(-2.2) 		629
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176400_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19419
Sequence Type Exemplar sequence
Representative Public ID Y57E12B.A NCBI
Target Description Y57E12B.A /REP_DB=WormBase Gene ID /WP=CE26550 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072355(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072355 NCBI Caenorhabditis elegans Y57E12B.4 (Y57E12B.4) mRNA, complete cds. 10/11 A 
SNAP00000007823 ENSEMBL cdna:SNAP chromosome:CEL140:V:6948304:6950110:-1 10/11 None
Y57E12B.4 ENSEMBL cdna:known chromosome:CEL140:V:6948304:6952447:-1 gene:Y57E12B.4 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6948667-6952448(-) 97.1 100.0
Public Domain and Genome References
Gene Title
Ensembl Y57E12B.4
Entrez Gene 179082 Entrez gene
SwissProt Q95XV0 EMBL-EBI
WormBase CE29892 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68538 Hypothetical protein Y57E12B.4 [Caenorhabditis elegans] ref|NP_504756.2| Y57E12B.4 [Caenorhabditis elegans] 0.0
blast AAK68538 Hypothetical protein Y57E12B.4 [Caenorhabditis elegans] ref|NP_504756.2| Y57E12B.4 [Caenorhabditis elegans] 1.0E-133
blast AAK68535 Hypothetical protein Y57E12B.1 [Caenorhabditis elegans] ref|NP_504757.1| Y57E12B.1 [Caenorhabditis elegans] 3.0E-21
blast XP_800520 PREDICTED: hypothetical protein XP_795427, partial [Strongylocentrotus purpuratus] 4.0E-14
blast AAN09644 CG32644-PB [Drosophila melanogaster] ref|NP_727652.1| CG32644-PB [Drosophila melanogaster] 1.0E-13
blast XP_800520 PREDICTED: hypothetical protein XP_795427, partial [Strongylocentrotus purpuratus] 4.0E-11
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504756.2 2 5-27,62-84
Y57E12B.4 2 5-27,62-84
Sequence
Target Sequence 
>C. ELEGANS:176400_AT
acacctcaagtgtcatcttctgagccattcactacttcggaaaatagtagtacagcagag
ccgttcgcaccttcggattcaactacttcaataaatgaaactcaaactacttcaacgcat
gctgaaacaacacctgaaattcactacacagagaaacccgcaacactcgaaccttcaaag
gcgccaaccgacgactgcccaacaactcttgttgtggagaataaaacactgactcctact
gagcacaagcctaccgaacattctacaacaattgaaaataccgaaccaacaactggaagg
gatagaacaacagatccagttatttcggacaaaactgagacaccatctacaaattttagc
acagttgcagaaaatacagcatcatcaacaccaatttatataactaaagttcctaccaca
tttgaaagatccgaaccaacaatgggtactgaggcaacaacagatcctgtttttccggac
aaaaccacaaatttcgccacagtttctgaacattcaagatcgtcaacaccaaattatatt
actgaacatcccaccacatttgccatgaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACCTCAAGTGTCATCTTCTGAGC 537 109 514 Antisense
TTCTGAGCCATTCACTACTTCGGAA 311 693 531 Antisense
AGTAGTACAGCAGAGCCGTTCGCAC 669 61 559 Antisense
CACCTTCGGATTCAACTACTTCAAT 281 197 581 Antisense
AACTCAAACTACTTCAACGCATGCT 696 139 612 Antisense
ACTACACAGAGAAACCCGCAACACT 71 101 656 Antisense
GCAACACTCGAACCTTCAAAGGCGC 570 317 673 Antisense
ACGACTGCCCAACAACTCTTGTTGT 391 91 704 Antisense
ACTGACTCCTACTGAGCACAAGCCT 363 95 741 Antisense
ACAAGCCTACCGAACATTCTACAAC 602 111 758 Antisense
ATCCCACCACATTTGCCATGAAAAA 563 37 1061 Antisense
Sequence Source Affymetrix Proprietary Database