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[176380_at]

Description Y37E11C.B /REP_DB=WormBase Gene ID /WP=CE26068 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 46      4grp_total > 27      6grp_cgc > 16      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 68.4
(-3)
A 		152.3
(3)
A 		77.6
(-2.3)
A 		147.1
(2.6)
A 		84
B 48.2
(-0.4)
A 		85.9
(3)
A 		19.9
(-3)
A 		47.6
(-0.5)
A 		66
C 68.1
(-3)
A 		81.8
(-1.8)
A 		86
(-1.5)
A 		139.5
(3)
A 		71
avr 61.6
(-3) 		106.7
(2.4) 		61.2
(-3) 		111.4
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 74.5
(3)
A 		48.8
(0.4)
A 		49.2
(0.4)
A 		58.5
(1.4)
A 		15.4
(-3)
A 		59.1
(1.4)
A 		59
B 62.5
(3)
A 		54.3
(1.4)
A 		36.9
(-1.9)
A 		35.8
(-2.2)
A 		31.4
(-3)
A 		51.4
(0.9)
A 		31
C 51.1
(-0.4)
A 		80.6
(2)
A 		66
(0.8)
A 		17.8
(-3)
A 		33.4
(-1.8)
A 		93.5
(3)
A 		76
avr 62.7
(2.2) 		61.2
(2) 		50.7
(0.5) 		37.4
(-1.4) 		26.7
(-3) 		68
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 24.9
(-0.8)
A 		33.1
(3)
A 		20.3
(-3)
A 		21.5
(-2.4)
A 		13
B 15.4
(0.9)
A 		21.2
(3)
A 		4.6
(-3)
A 		10.1
(-1)
A 		17
C 22.5
(-3)
A 		23.5
(-2.2)
A 		30
(3)
A 		23.5
(-2.2)
A 		8
avr 20.9
(-0.9) 		25.9
(3) 		18.3
(-3) 		18.4
(-2.9) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 13.7
(1.3)
A 		17.3
(3)
A 		10.7
(-0.1)
A 		14.8
(1.8)
A 		4.7
(-3)
A 		14.4
(1.6)
A 		13
B 21.8
(3)
A 		18.1
(1.3)
A 		9
(-3)
A 		9.5
(-2.8)
A 		12.7
(-1.3)
A 		19.2
(1.8)
A 		13
C 18.9
(0.3)
A 		24.6
(1.5)
A 		16.8
(-0.1)
A 		3.6
(-3)
A 		10.8
(-1.4)
A 		31.3
(3)
A 		28
avr 18.1
(1.3) 		20
(2.2) 		12.2
(-1.6) 		9.3
(-3) 		9.4
(-3) 		21.6
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176380_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19259
Sequence Type Exemplar sequence
Representative Public ID Y37E11C.B NCBI
Target Description Y37E11C.B /REP_DB=WormBase Gene ID /WP=CE26068 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171303(11), NM_171304(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171303 NCBI Caenorhabditis elegans UNCoordinated family member (unc-33) (unc-33) mRNA, complete cds. 11/11 A 
NM_171304 NCBI Caenorhabditis elegans UNCoordinated family member (unc-33) (unc-33) mRNA, complete cds. 9/11 None
Y37E11C.1a ENSEMBL cdna:known chromosome:CEL140:IV:3516818:3526598:1 gene:Y37E11C.1 11/11 A 
Y37E11C.1b ENSEMBL cdna:known chromosome:CEL140:IV:3518144:3525568:1 gene:Y37E11C.1 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011888 5/11 Cross Hyb Matching Probes None
GENEFINDER00000011896 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177112 3 2 NM_171303 176380_at (11)
NM_171304 176380_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3516888-3519779(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-33
Ensembl Y37E11C.1
Entrez Gene 177112 Entrez gene
SwissProt Q01630 EMBL-EBI
WormBase CE21557 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA78520 hypothetical polypeptide I [Caenorhabditis elegans] emb|CAA78516.1| unc-33 [Caenorhabditis elegans] ref|NP_741357.1| UNCoordinated family member (unc-33) [Caenorhabditis elegans] gb|AAM97965.1| Uncoordinated protein 33 [Caenorhabditis elegans] sp|Q01630|UNC33_CAEEL Uncoordinated protein 33 (Protein unc-33) 0.0
blast CAA78521 hypothetical polypeptide II [Caenorhabditis elegans] emb|CAA78517.1| hypothetical [Caenorhabditis elegans] ref|NP_741358.1| UNCoordinated family member (unc-33) [Caenorhabditis elegans] 0.0
blast CAA78522 hypothetical polypeptide III [Caenorhabditis elegans] emb|CAA78518.1| hypothetical [Caenorhabditis elegans] ref|NP_741359.1| UNCoordinated family member (unc-33) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.9E-11
Sequence
Target Sequence 
>C. ELEGANS:176380_AT
agcaatgctaaactctctcagcctggcccagaatggtttgaagggttcgaacagatggat
atgacggatattgaactaccccccgacccgaactgcccggcggaaaaggtgttgcgcgga
gaaaaatcgacgccagacttcgattcggattggcaagaagcgaaagaagacgttttagat
cctcaaagctacccgaaaagcttcaaccccgccgaatcgcttcccggtccagatattggc
gccggcgttgacgatgaggaagagcctgaggctgaagctcaagaaatggaagagcctcaa
tacgaatcgaaagtggacgaaaaagacgacgacgacaatggctcttcttcctccaaaaag
ggtcatccttccgaagacggagatagtacaagaaatggagagacgccgacagaccgacga
aatagtggggcgattgaagagacggcagacggtgagagctctgcgcagtcggctgctgag
aag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAATGCTAAACTCTCTCAGCCTG 540 75 490 Antisense
CTCTCAGCCTGGCCCAGAATGGTTT 304 231 504 Antisense
TGACGGATATTGAACTACCCCCCGA 511 569 551 Antisense
ACTGCCCGGCGGAAAAGGTGTTGCG 42 99 581 Antisense
GACGCCAGACTTCGATTCGGATTGG 491 379 618 Antisense
GTTTTAGATCCTCAAAGCTACCCGA 222 425 661 Antisense
CGCTTCCCGGTCCAGATATTGGCGC 336 253 707 Antisense
ATATTGGCGCCGGCGTTGACGATGA 313 19 722 Antisense
AGGGTCATCCTTCCGAAGACGGAGA 153 53 848 Antisense
AGACGCCGACAGACCGACGAAATAG 579 57 890 Antisense
CTCTGCGCAGTCGGCTGCTGAGAAG 19 231 948 Antisense
Sequence Source Affymetrix Proprietary Database