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[176375_at]

Description Y38F2AR.G /REP_DB=WormBase Gene ID /WP=CE21616 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 07      4grp_total > 53      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 273.5
(3)
A 		195.2
(0.3)
A 		144
(-1.5)
A 		102.2
(-3)
A 		171
B 254.6
(3)
A 		211.7
(1.8)
A 		220.6
(2.1)
A 		39.8
(-3)
A 		215
C 315.6
(3)
A 		259.7
(1.4)
A 		190.3
(-0.5)
A 		99.3
(-3)
A 		216
avr 281.2
(3) 		222.2
(1.2) 		185
(0.1) 		80.4
(-3) 		201

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 226
1 2 3 4 5 6 range
A 163
(1.8)
A 		186
(3)
A 		129.7
(0.1)
A 		104
(-1.3)
A 		71.1
(-3)
A 		101.8
(-1.4)
A 		115
B 159.1
(3)
A 		98.3
(-2.1)
A 		105.5
(-1.5)
A 		148.2
(2.1)
A 		87.8
(-3)
A 		95.1
(-2.4)
A 		71
C 243.7
(3)
A 		94.2
(-1)
A 		144.3
(0.4)
A 		17.4
(-3)
A 		104.4
(-0.7)
A 		91.4
(-1)
A 		226
avr 188.6
(3) 		126.2
(-0.7) 		126.5
(-0.7) 		89.9
(-2.9) 		87.8
(-3) 		96.1
(-2.5) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 99.6
(3)
A 		42.4
(-1.1)
A 		37.6
(-1.4)
A 		15
(-3)
A 		85
B 81.2
(3)
A 		52.3
(0.6)
A 		51.1
(0.5)
A 		8.5
(-3)
A 		73
C 104.5
(3)
A 		74.7
(1)
A 		66.3
(0.4)
A 		16.7
(-3)
A 		88
avr 95.1
(3) 		56.5
(0.2) 		51.7
(-0.2) 		13.4
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 30
(-1.9)
A 		66.1
(3)
A 		28.2
(-2.1)
A 		26.4
(-2.3)
A 		21.5
(-3)
A 		24.8
(-2.6)
A 		45
B 55.3
(3)
A 		32.7
(-1.6)
A 		25.6
(-3)
A 		39.2
(-0.3)
A 		35.7
(-1)
A 		35.5
(-1)
A 		30
C 90.3
(3)
A 		28.8
(-1.3)
A 		36.7
(-0.7)
A 		3.5
(-3)
A 		33.8
(-0.9)
A 		30.6
(-1.1)
A 		87
avr 58.5
(3) 		42.5
(0.3) 		30.2
(-1.8) 		23
(-3) 		30.3
(-1.8) 		30.3
(-1.8) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176375_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19143
Sequence Type Exemplar sequence
Representative Public ID Y38F2AR.G NCBI
Target Description Y38F2AR.G /REP_DB=WormBase Gene ID /WP=CE21616 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067791(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067791 NCBI Caenorhabditis elegans Y38F2AR.8 (Y38F2AR.8) mRNA, complete cds. 11/11 None
GENEFINDER00000033677 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2347428:2348910:-1 11/11 None
Y38F2AR.8 ENSEMBL cdna:known chromosome:CEL140:IV:2344976:2348911:-1 gene:Y38F2AR.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075647 3/11 Cross Hyb Matching Probes None
GENEFINDER00000014622 3/11 Cross Hyb Matching Probes None
Y35H6.1 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:34587-35513(+) 72.81 72.81
chrIV:2347433-2349128(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y38F2AR.8
Entrez Gene 3565495 Entrez gene
SwissProt Q95XS7 EMBL-EBI
WormBase CE37283 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_500192 Y38F2AR.8 [Caenorhabditis elegans] gb|AAK68449.2| Hypothetical protein Y38F2AR.8 [Caenorhabditis elegans] 1.0E-157
blast NP_500192 Y38F2AR.8 [Caenorhabditis elegans] gb|AAK68449.2| Hypothetical protein Y38F2AR.8 [Caenorhabditis elegans] 4.0E-98
blast AAK31562 Hypothetical protein Y35H6.1 [Caenorhabditis elegans] ref|NP_508048.1| Y35H6.1 [Caenorhabditis elegans] 6.0E-54
blast AAK31562 Hypothetical protein Y35H6.1 [Caenorhabditis elegans] ref|NP_508048.1| Y35H6.1 [Caenorhabditis elegans] 1.0E-53
blast XP_694729 PREDICTED: similar to Spg7 protein, partial [Danio rerio] 3.0E-22
blast BAE26494.1 unnamed protein product [Mus musculus] 8.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR005936 EMBL-EBI Peptidase M41, FtsH 3.8E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500192.2 2 111-133,226-245
Y38F2AR.8 2 111-133,226-245
GENEFINDER00000033677 1 111-133
Sequence
Target Sequence 
>C. ELEGANS:176375_AT
gagtgctcgcagatttccacaaaggaggcccgctcgcccgaaggaagaaagttcagaagc
agagaaaagtccaccaaaacgctgggaattcgagataagctggagctccaaaggccaccg
agaaacaaaatccgacgcaaaatcagcgtcagaaggcaatgaaaacggcgggggaaataa
gggcgataatgaccctcgcaagcaattaatggaaaaaatgaagagatggcttgcaatttc
actttttgcctacggaattctatttattttatctccaaaaggagccgctgctgtcacagc
tgataaaatttcatggagtgaatttataaatgagctattgccaactggacaagtttatcg
aattattattctaccggaaaaggatgtcgcatatctttattgctatgatactggagctaa
gaattcgagaggtgaaaagctggcgagcatgtacagagtcggtattccttccgtcgcaag
attcgaaacagaagttagagctgctgaagctgctctcgggcttccgccagagcattggac
acaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTGCTCGCAGATTTCCACAAAGG 91 401 153 Antisense
GCCCGCTCGCCCGAAGGAAGAAAGT 157 285 180 Antisense
ATAAGCTGGAGCTCCAAAGGCCACC 587 21 247 Antisense
AGGGCGATAATGACCCTCGCAAGCA 348 57 332 Antisense
GAGATGGCTTGCAATTTCACTTTTT 562 395 375 Antisense
TTCACTTTTTGCCTACGGAATTCTA 297 685 390 Antisense
TCCAAAAGGAGCCGCTGCTGTCACA 592 587 426 Antisense
GTACAGAGTCGGTATTCCTTCCGTC 617 459 603 Antisense
TATTCCTTCCGTCGCAAGATTCGAA 49 663 615 Antisense
TAGAGCTGCTGAAGCTGCTCTCGGG 246 653 648 Antisense
GCTTCCGCCAGAGCATTGGACACAA 274 307 672 Antisense
Sequence Source Affymetrix Proprietary Database