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[176350_s_at]

Description Y22D7AL.N /REP_DB=WormBase Gene ID /WP=CE26501 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 82      6grp_total > 28      4grp_total > 28      6grp_highest_sub > Cu      6grp_cgc > 03      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 398.1
(0)
P 		383.4
(-0.5)
P 		302.4
(-3)
P 		493.1
(3)
P 		191
B 464.6
(3)
P 		376.2
(-0.7)
P 		320.7
(-3)
P 		462.3
(2.9)
P 		144
C 364.4
(-3)
P 		440.8
(3)
P 		387
(-1.2)
P 		405.2
(0.2)
P 		76
avr 409
(0.7) 		400.1
(0.3) 		336.7
(-3) 		453.5
(3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 467.6
(3)
P 		357.7
(-1.4)
P 		389.3
(-0.1)
P 		316.6
(-3)
P 		364.4
(-1.1)
P 		363.2
(-1.1)
P 		151
B 485.6
(3)
P 		432.7
(-0.4)
P 		392.1
(-3)
P 		395.9
(-2.7)
P 		425.5
(-0.8)
P 		391.7
(-3)
P 		94
C 426.2
(3)
P 		423.7
(2.9)
P 		319
(-3)
P 		356.6
(-0.9)
P 		388.8
(0.9)
P 		416.7
(2.5)
P 		107
avr 459.8
(3) 		404.7
(-0.2) 		366.8
(-2.4) 		356.4
(-3) 		392.9
(-0.9) 		390.5
(-1) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 144.9
(3)
P 		83.2
(-2.1)
P 		79.1
(-2.4)
P 		72.2
(-3)
P 		73
B 148.2
(3)
P 		92.9
(-1.5)
P 		74.3
(-3)
P 		98.5
(-1)
P 		74
C 120.6
(1.7)
P 		126.7
(2.3)
P 		134.9
(3)
P 		68.3
(-3)
P 		67
avr 137.9
(3) 		100.9
(-0.8) 		96.1
(-1.3) 		79.7
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 86.2
(-2.2)
P 		127.2
(3)
P 		84.8
(-2.4)
P 		80.3
(-3)
P 		110
(0.8)
P 		88.4
(-2)
P 		47
B 169
(2.7)
P 		144
(0.8)
P 		95.2
(-3)
P 		104.9
(-2.3)
P 		172.9
(3)
P 		146.1
(0.9)
P 		78
C 158
(3)
P 		129.3
(1)
P 		81.2
(-2.3)
P 		71.7
(-3)
P 		125.8
(0.8)
P 		139.5
(1.7)
P 		86
avr 137.7
(3) 		133.5
(2.5) 		87.1
(-2.8) 		85.6
(-3) 		136.2
(2.8) 		124.7
(1.5) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176350_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19057
Sequence Type Exemplar sequence
Representative Public ID Y22D7AL.N NCBI
Target Description Y22D7AL.N /REP_DB=WormBase Gene ID /WP=CE26501 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065024(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065024 NCBI Caenorhabditis elegans SphingoMyelin Synthase family member (sms-3) (sms-3) mRNA, complete cds. 11/11 None
GENEFINDER00000002279 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1583459:1600273:-1 11/11 None
Y22D7AL.8 ENSEMBL cdna:known chromosome:CEL140:III:1583159:1589392:-1 gene:Y22D7AL.8 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1583458-1589392(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sms-3
Ensembl Y22D7AL.8
Entrez Gene 175313 Entrez gene
SwissProt Q965Q4 EMBL-EBI
WormBase CE26501 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84597 Sphingomyelin synthase protein 3 [Caenorhabditis elegans] ref|NP_497425.1| SphingoMyelin Synthase family member (sms-3) [Caenorhabditis elegans] sp|Q965Q4|SMS3_CAEEL Putative phosphatidylcholine:ceramide cholinephosphotransferase 3 (Sphingomyelin synthase 3) 0.0
blast CAE66426 Hypothetical protein CBG11695 [Caenorhabditis briggsae] 1.0E-157
blast CAE66426 Hypothetical protein CBG11695 [Caenorhabditis briggsae] 1.0E-156
blast AAA82341 Sphingomyelin synthase protein 2 [Caenorhabditis elegans] ref|NP_508182.1| SphingoMyelin Synthase family member (sms-2) [Caenorhabditis elegans] sp|Q20735|SMS2_CAEEL Putative phosphatidylcholine:ceramide cholinephosphotransferase 2 (Sphingomyelin synthase 2) 3.0E-37
blast AAA82341 Sphingomyelin synthase protein 2 [Caenorhabditis elegans] ref|NP_508182.1| SphingoMyelin Synthase family member (sms-2) [Caenorhabditis elegans] sp|Q20735|SMS2_CAEEL Putative phosphatidylcholine:ceramide cholinephosphotransferase 2 (Sphingomyelin synthase 2) 4.0E-37
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497425.1 4 37-59,79-101,178-199,214-236
Y22D7AL.8 4 37-59,79-101,178-199,214-236
GENEFINDER00000002279 4 152-174,194-216,293-314,329-351
Sequence
Target Sequence 
>C. ELEGANS:176350_S_AT
taccctcggggtacacggataatcaggggagatgccgggatcaagtggagtcagaagctg
gtgttttctttgggaggctctttgagcagacgattaggatcgggtttcagagcaaagatc
aaatgctatgtggagacctacttttctccggtcacacacttgtgatggtcacctgctctt
tggccgtcgcctattacctcccaaaatcgataaaaccactgcaatgggtgagccacgtgg
catgtttgattgggatgatatgtatgacgatatcacggactcattatacaattgatgtgg
ttattgcatattggttgagcaatatggtttttagaatgtaccacgcgtactgtgaggtag
acatgtgcatggaacggcgaaaaagtatactgtattcgtggtggccgtgccgtattgttg
attggctcgagcaggatattgtgcccggtcgtctggagaatcgttgtcagcttccgtggc
ggcgctcgacgccgcgcggccaagaaaggggcggagccagcgcggagtcatcggactcgt
cggttacaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACCCTCGGGGTACACGGATAATCA 392 649 407 Antisense
GAAGCTGGTGTTTTCTTTGGGAGGC 101 341 460 Antisense
TGTGGAGACCTACTTTTCTCCGGTC 475 555 535 Antisense
ACACTTGTGATGGTCACCTGCTCTT 499 109 562 Antisense
GCCTATTACCTCCCAAAATCGATAA 190 281 595 Antisense
ACCACTGCAATGGGTGAGCCACGTG 664 85 621 Antisense
TATACTGTATTCGTGGTGGCCGTGC 524 659 792 Antisense
GGCCGTGCCGTATTGTTGATTGGCT 278 543 809 Antisense
CGAGCAGGATATTGTGCCCGGTCGT 374 249 834 Antisense
CTGGAGAATCGTTGTCAGCTTCCGT 249 241 859 Antisense
GTCATCGGACTCGTCGGTTACAATG 366 461 933 Antisense
Sequence Source Affymetrix Proprietary Database