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[176346_s_at]

Description Y38C1AB.9 /REP_DB=WormBase Gene ID /WP=CE26071 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 4grp_high_8_8 > 44      6grp_total > 34      4grp_total > 52      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 93.5
(1.8)
P 		100.3
(2.3)
P 		110.2
(3)
P 		29.8
(-3)
A 		80
B 127.1
(3)
P 		103.8
(0.3)
P 		76
(-3)
P 		77.5
(-2.8)
P 		51
C 117.2
(-0.1)
P 		178.1
(3)
P 		131.6
(0.6)
P 		60.1
(-3)
P 		118
avr 112.6
(1.8) 		127.4
(3) 		105.9
(1.2) 		55.8
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 81.6
(0.6)
A 		114.3
(3)
P 		67.3
(-0.5)
P 		34
(-3)
A 		50.5
(-1.8)
A 		49.2
(-1.9)
A 		80
B 95.1
(1.9)
P 		68.4
(-0.2)
P 		109.3
(3)
P 		65
(-0.4)
A 		39.7
(-2.4)
A 		31.6
(-3)
A 		78
C 134.2
(3)
P 		69.5
(-1.3)
P 		97.1
(0.5)
P 		44.1
(-3)
A 		109.3
(1.3)
A 		52.9
(-2.4)
A 		90
avr 103.6
(3) 		84.1
(1) 		91.2
(1.7) 		47.7
(-2.7) 		66.5
(-0.8) 		44.6
(-3) 		59
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 34
(3)
P 		21.8
(0.5)
P 		28.8
(1.9)
P 		4.4
(-3)
A 		30
B 40.5
(3)
P 		25.6
(-0.7)
P 		17.6
(-2.7)
P 		16.5
(-3)
P 		24
C 38.8
(1.2)
P 		51.2
(3)
P 		45.9
(2.2)
P 		10.1
(-3)
P 		41
avr 37.8
(3) 		32.9
(1.9) 		30.8
(1.5) 		10.3
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 15
(-1.8)
A 		40.7
(3)
P 		14.7
(-1.9)
P 		8.6
(-3)
A 		15.2
(-1.8)
A 		12
(-2.4)
A 		32
B 33.1
(3)
P 		22.8
(0.1)
P 		26.5
(1.1)
P 		17.2
(-1.5)
A 		16.1
(-1.8)
A 		11.8
(-3)
A 		21
C 49.7
(3)
P 		21.2
(-1.2)
P 		24.7
(-0.7)
P 		8.9
(-3)
A 		35.4
(0.9)
A 		17.7
(-1.7)
A 		41
avr 32.6
(3) 		28.2
(1.7) 		22
(-0) 		11.6
(-3) 		22.2
(0) 		13.8
(-2.4) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176346_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10578
Sequence Type Exemplar sequence
Representative Public ID Y38C1AB.9 NCBI
Target Description Y38C1AB.9 /REP_DB=WormBase Gene ID /WP=CE26071 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067449(11), NM_067455(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067449 NCBI Caenorhabditis elegans Y38C1AB.4 (Y38C1AB.4) mRNA, complete cds. 11/11 A 
NM_067455 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-5) (frm-5) mRNA, complete cds. 11/11 A 
SNAP00000032132 ENSEMBL cdna:SNAP chromosome:CEL140:IV:644:9937:1 11/11 None
GENEFINDER00000032150 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:695:8548:1 11/11 None
Y38C1AB.4 ENSEMBL cdna:known chromosome:CEL140:IV:695:14926:1 gene:Y38C1AB.4 11/11 A 
Y38C1AB.8 ENSEMBL cdna:known chromosome:CEL140:IV:32953:47184:-1 gene:Y38C1AB.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6271-8838(+) 99.66 100.0
chrIV:39040-41607(-) 99.66 100.0
Public Domain and Genome References
Gene Title

Gene Symbol frm-5
Ensembl Y38C1AB.4
Y38C1AB.8
Entrez Gene 176821 Entrez gene
176822 Entrez gene
SwissProt Q9N531 EMBL-EBI
Q9N530 EMBL-EBI
WormBase CE25254 Wormbase
CE27782 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5856 cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60499 Hypothetical protein Y38C1AB.4 [Caenorhabditis elegans] ref|NP_499850.1| Y38C1AB.4 [Caenorhabditis elegans] 0.0
blast CAE58494 Hypothetical protein CBG01639 [Caenorhabditis briggsae] 0.0
blast AAF60494 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 5 [Caenorhabditis elegans] ref|NP_499856.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-5) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Sequence
Target Sequence 
>C. ELEGANS:176346_S_AT
acggatcatcactcgaaatcctcctatttcgtgcgattcgccgcctcccaacatcgttgg
atgatgaagatgcgtcagtggaagagcacgctccggcacgagaatacgattcaggcgatg
ccagacgtgattgttgaagggcagactatcccgccggcgcctatacggcaaggtctcgaa
gaatcacctccatcaactccactgctcgcctctgccgatcagatctttgcgaaaatgtca
gtgagccaggaaaaaccggcggccaaccgacccgccgaactgccaccaccggcaccgtcg
tcgaaattcgcggctcaatatgatacggttgatgaaggaattgtttgtgattcccaagct
gaaaactttgagcgcgtcgactcgactgacaacgggaacgtgacgccacgtggcatgcaa
tttgacattttgctcgtgaaagaccccgccaacggtctgggtcttaccctagtcgacggg
aatctcaacggagttccaggagtgtatgtgaagcttgtcgccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGATCATCACTCGAAATCCTCCT 471 95 571 Antisense
GAAATCCTCCTATTTCGTGCGATTC 628 361 585 Antisense
TCAGTGGAAGAGCACGCTCCGGCAC 533 623 645 Antisense
CAGGCGATGCCAGACGTGATTGTTG 311 209 682 Antisense
TGTTGAAGGGCAGACTATCCCGCCG 109 543 702 Antisense
GCGCCTATACGGCAAGGTCTCGAAG 322 287 727 Antisense
CCTCTGCCGATCAGATCTTTGCGAA 710 267 779 Antisense
TGAGCGCGTCGACTCGACTGACAAC 554 569 939 Antisense
ACGCCACGTGGCATGCAATTTGACA 505 91 973 Antisense
GTCTTACCCTAGTCGACGGGAATCT 215 467 1031 Antisense
AGTGTATGTGAAGCTTGTCGCCGAC 413 59 1071 Antisense
Sequence Source Affymetrix Proprietary Database