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[176345_s_at]

Description Y22D7AL.Q /REP_DB=WormBase Gene ID /WP=CE26504 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 30      6grp_total > 06      4grp_total > 58      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1975
1 2 3 4 range
A 1206.9
(-0.4)
P 		1580.5
(0.9)
P 		2215.1
(3)
P 		420.5
(-3)
P 		1795
B 1150.1
(-0.5)
P 		1598.9
(0.9)
P 		2238.9
(3)
P 		397.2
(-3)
P 		1842
C 1179.4
(-0.6)
P 		2231.2
(2.6)
P 		2359.9
(3)
P 		385
(-3)
P 		1975
avr 1178.8
(-0.5) 		1803.5
(1.5) 		2271.3
(3) 		400.9
(-3) 		1870

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1876
1 2 3 4 5 6 range
A 788.2
(-0.4)
P 		1310.5
(3)
P 		570.3
(-1.8)
P 		801.1
(-0.3)
P 		385.5
(-3)
P 		702.8
(-0.9)
P 		925
B 859.6
(3)
P 		711.9
(1.1)
P 		732.7
(1.4)
P 		500.3
(-1.5)
P 		483.4
(-1.8)
P 		384.4
(-3)
P 		475
C 2234.5
(3)
P 		686.1
(-2)
P 		924.7
(-1.2)
P 		464.2
(-2.7)
P 		602.8
(-2.2)
P 		358.4
(-3)
P 		1876
avr 1294.1
(3) 		902.8
(0.1) 		742.6
(-1.1) 		588.5
(-2.2) 		490.6
(-2.9) 		481.9
(-3) 		812
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 761
1 2 3 4 range
A 439.4
(1.4)
P 		343.1
(0.3)
P 		579.1
(3)
P 		61.6
(-3)
P 		518
B 366.7
(0.9)
P 		394.9
(1.3)
P 		518.8
(3)
P 		84.6
(-3)
P 		434
C 390.4
(-0.4)
P 		641.5
(1.6)
P 		822.5
(3)
P 		64.9
(-3)
P 		758
avr 398.8
(0.5) 		459.8
(1.1) 		640.1
(3) 		70.4
(-3) 		570

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 735
1 2 3 4 5 6 range
A 145.3
(-2.5)
P 		466.1
(3)
P 		124.2
(-2.9)
P 		203.2
(-1.5)
P 		116.3
(-3)
P 		171.1
(-2.1)
P 		350
B 299.1
(3)
P 		237
(0.8)
P 		177.9
(-1.4)
P 		132.5
(-3)
P 		196.4
(-0.7)
P 		143.3
(-2.6)
P 		167
C 828.2
(3)
P 		209.3
(-2.1)
P 		235.3
(-1.8)
P 		93.4
(-3)
P 		195.1
(-2.2)
P 		120
(-2.8)
P 		735
avr 424.2
(3) 		304.1
(0.4) 		179.1
(-2.2) 		143
(-3) 		169.3
(-2.4) 		144.8
(-3) 		281
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176345_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19048
Sequence Type Exemplar sequence
Representative Public ID Y22D7AL.Q NCBI
Target Description Y22D7AL.Q /REP_DB=WormBase Gene ID /WP=CE26504 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065028(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065028 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-60) (hsp-60) mRNA, complete cds. 11/11 A 
GENEFINDER00000002278 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1619748:1625395:1 11/11 None
Y22D7AL.5.3 ENSEMBL cdna:known chromosome:CEL140:III:1619731:1625774:1 gene:Y22D7AL.5 11/11 None
Y22D7AL.5.2 ENSEMBL cdna:known chromosome:CEL140:III:1619734:1625780:1 gene:Y22D7AL.5 11/11 None
Y22D7AL.5.1 ENSEMBL cdna:known chromosome:CEL140:III:1619739:1625784:1 gene:Y22D7AL.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1619747-1622069(+) 92.7 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hsp-60
Ensembl Y22D7AL.5
Entrez Gene 175316 Entrez gene
SwissProt P50140 EMBL-EBI
WormBase CE27244 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245164_AT chaperonin, putative at
ATGENOME1:19430_AT chaperonin, putative at
DROSOPHILA_2:1641286_S_AT Heat shock protein 60 dm
DROSGENOME1:141664_AT Heat shock protein 60 dm
MU11KSUBB:MSA.42608.0_F_AT chaperonin mm
MU19KSUBB:TC32589_AT PREDICTED: similar to 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65) [Mus musculus], mRNA sequence mm
YEAST_2:1772196_AT Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; similarity to groEL Sc
Protein Similarities
Method ID Description E-Value
blast AAK84594 Heat shock protein protein 60 [Caenorhabditis elegans] ref|NP_497429.1| Heat Shock Protein family member (hsp-60) [Caenorhabditis elegans] sp|P50140|CH60_CAEEL Chaperonin homolog HSP60, mitochondrial precursor (Heat shock protein 60) (HSP-60) 0.0
blast AAA28077 homologous to chaperonin protein 0.0
blast CAE66431 Hypothetical protein CBG11701 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 2.9E-106
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 6.7E-101
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176345_S_AT
tcgccacaatttccgccaatggagacaccgtcgtcggaaatctcatcagtgacgccatga
agaaggtcggaaccactggagtcatcactgttaaggacggaaaaacgctgaacgatgagc
tcgagcttatcgagggaatgaagttcgacagaggatacatctcgccgtactttatcacct
ctgcgaaaggagctaaagtcgagtacgagaaggctcttgtcttgttgagcgagaagaaga
tcagccaagttcaggatattgtgccggctcttgagctcgccaacaagctccgccgtccat
tggttatcattgccgaggatgttgatggagaggctcttaccactcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGCCACAATTTCCGCCAATGGAGA 481 599 497 Antisense
GGAGACACCGTCGTCGGAAATCTCA 461 519 517 Antisense
CGGAAATCTCATCAGTGACGCCATG 580 241 531 Antisense
AACGCTGAACGATGAGCTCGAGCTT 645 145 600 Antisense
GACAGAGGATACATCTCGCCGTACT 339 367 643 Antisense
CGTACTTTATCACCTCTGCGAAAGG 199 247 662 Antisense
GTACGAGAAGGCTCTTGTCTTGTTG 361 459 699 Antisense
TTCAGGATATTGTGCCGGCTCTTGA 256 685 746 Antisense
GGCTCTTGAGCTCGCCAACAAGCTC 241 539 762 Antisense
GCCGTCCATTGGTTATCATTGCCGA 686 283 788 Antisense
GATGGAGAGGCTCTTACCACTCTTG 395 405 820 Antisense
Sequence Source Affymetrix Proprietary Database