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[176337_at]

Description Y50D4B.2 /REP_DB=WormBase Gene ID /WP=CE26133 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1232
1 2 3 4 range
A 67
(-2.7)
A 		10.4
(-3)
A 		8.4
(-3)
A 		1240
(3)
P 		1232
B 48
(-3)
A 		62
(-2.9)
A 		50.2
(-3)
A 		1058
(3)
P 		1010
C 17.3
(-3)
A 		45.2
(-2.8)
A 		54.8
(-2.8)
A 		1072
(3)
P 		1055
avr 44.1
(-3) 		39.2
(-3) 		37.8
(-3) 		1123.3
(3) 		1086

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1853
1 2 3 4 5 6 range
A 146.2
(-2.6)
A 		79.5
(-2.8)
A 		63
(-2.9)
A 		23.3
(-3)
A 		101.1
(-2.7)
A 		1864
(3)
P 		1841
B 150.2
(-2.5)
P 		52.6
(-2.9)
A 		73.9
(-2.8)
A 		13.5
(-3)
A 		69.7
(-2.8)
A 		1633.6
(3)
P 		1620
C 126.4
(-2.5)
A 		30.4
(-2.9)
A 		44.5
(-2.9)
A 		11.1
(-3)
A 		83.2
(-2.7)
A 		1425.9
(3)
P 		1415
avr 140.9
(-2.5) 		54.2
(-2.9) 		60.5
(-2.8) 		16
(-3) 		84.7
(-2.7) 		1641.2
(3) 		1625
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 24.4
(-2.3)
A 		2.3
(-3)
A 		2.2
(-3)
A 		181.5
(3)
P 		179
B 15.3
(-2.9)
A 		15.3
(-2.9)
A 		11.6
(-3)
A 		225.5
(3)
P 		214
C 5.7
(-3)
A 		13
(-2.7)
A 		19.1
(-2.5)
A 		180.8
(3)
P 		175
avr 15.1
(-2.8) 		10.2
(-3) 		11
(-3) 		195.9
(3) 		186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 607
1 2 3 4 5 6 range
A 27
(-2.7)
A 		28.3
(-2.7)
A 		13.7
(-2.9)
A 		5.9
(-3)
A 		30.5
(-2.7)
A 		453.8
(3)
P 		448
B 52.3
(-2.5)
P 		17.5
(-2.9)
A 		17.9
(-2.9)
A 		3.6
(-3)
A 		28.3
(-2.8)
A 		609.2
(3)
P 		606
C 46.9
(-2.4)
A 		9.3
(-2.9)
A 		11.3
(-2.9)
A 		2.2
(-3)
A 		26.9
(-2.7)
A 		477.3
(3)
P 		475
avr 42.1
(-2.6) 		18.4
(-2.8) 		14.3
(-2.9) 		3.9
(-3) 		28.6
(-2.7) 		513.4
(3) 		510
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176337_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15666
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.2 NCBI
Target Description Y50D4B.2 /REP_DB=WormBase Gene ID /WP=CE26133 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070978(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070978 NCBI Caenorhabditis elegans Y50D4B.2 (Y50D4B.2) mRNA, complete cds. 11/11 None
SNAP00000024550 ENSEMBL cdna:SNAP chromosome:CEL140:V:1101741:1103876:1 11/11 None
GENEFINDER00000024552 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1088199:1103876:1 11/11 None
Y50D4B.2 ENSEMBL cdna:known chromosome:CEL140:V:1101837:1103876:1 gene:Y50D4B.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1100981-1103876(+) 89.8 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.2
Entrez Gene 190094 Entrez gene
SwissProt Q9N4S9 EMBL-EBI
WormBase CE24401 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84612 Hypothetical protein Y50D4B.4 [Caenorhabditis elegans] ref|NP_503377.2| Y50D4B.4 [Caenorhabditis elegans] 0.0
blast AAF59494 Hypothetical protein Y50D4B.5 [Caenorhabditis elegans] ref|NP_503376.1| Y50D4B.5 [Caenorhabditis elegans] 1.0E-174
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 1.0E-168
blast AAF59496 Hypothetical protein Y50D4B.2 [Caenorhabditis elegans] ref|NP_503379.3| Y50D4B.2 [Caenorhabditis elegans] 1.0E-124
blast AAF59496 Hypothetical protein Y50D4B.2 [Caenorhabditis elegans] ref|NP_503379.3| Y50D4B.2 [Caenorhabditis elegans] 1.0E-123
blast AAF99915 Hypothetical protein F22F7.1a [Caenorhabditis elegans] ref|NP_503577.1| F22F7.1a [Caenorhabditis elegans] 4.0E-90
blast CAE71032 Hypothetical protein CBG17873 [Caenorhabditis briggsae] 1.0E-88
blast AAF99915 Hypothetical protein F22F7.1a [Caenorhabditis elegans] ref|NP_503577.1| F22F7.1a [Caenorhabditis elegans] 9.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.1E-31
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000024552 3 38-60,70-92,402-424
Sequence
Target Sequence 
>C. ELEGANS:176337_AT
tattccaccgcgtcgaggagttgtctggaaaattgaagaaaaagagcttcaaggctttgc
catcccattccctggtgctgatctatcagtgatggtccggagtcaatattatgatgcgac
tgttcgaaaatctcgtcctgttcatctttctagttactttcaacttgacactcgattcaa
gacgtatcgcctgattctctggggtgctatcacgcgcgcctttgctcttgcttcattcac
tcgccgaatccttttgaaatacccggatcagtgcagtttccgaatgttcaagaaatcggg
tccaagtgctcaacaaattgctgaagcgtcattcacgtattggttctttggatatggata
caaggagactattccactggatcagcagcacgagagaaaagtcgatagaaaagtggtggc
tacttgtaagggaccagatctcgcatatatggcagctagtggttgtgttctttccgctgc
actagcattggttaaagataagaacaacctgccaaaggaaggaggcgtctacactccagc
cgctgcttttggagactccaagatatacgattacctggcaacctttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTCCACCGCGTCGAGGAGTTGTC 387 661 516 Antisense
AAGAGCTTCAAGGCTTTGCCATCCC 80 155 557 Antisense
GCGACTGTTCGAAAATCTCGTCCTG 2 291 631 Antisense
GTCCTGTTCATCTTTCTAGTTACTT 52 477 650 Antisense
TCAAGACGTATCGCCTGATTCTCTG 208 631 692 Antisense
ATTCACTCGCCGAATCCTTTTGAAA 662 11 750 Antisense
GAAATCGGGTCCAAGTGCTCAACAA 247 361 807 Antisense
GCGTCATTCACGTATTGGTTCTTTG 50 293 841 Antisense
GTAAGGGACCAGATCTCGCATATAT 26 463 941 Antisense
CTTTCCGCTGCACTAGCATTGGTTA 550 223 985 Antisense
ATACGATTACCTGGCAACCTTTGGA 336 23 1080 Antisense
Sequence Source Affymetrix Proprietary Database