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[176335_at]

Description F28A10.9 /REP_DB=WormBase Gene ID /WP=CE19414 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 11      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 3.4
(-2.8)
A 		11.2
(0.5)
A 		2.9
(-3)
A 		17.1
(3)
A 		14
B 30.5
(3)
A 		3.4
(-2.7)
A 		3.3
(-2.7)
A 		2.1
(-3)
A 		28
C 3.6
(-3)
A 		14.2
(-2.4)
A 		3.8
(-3)
A 		108.5
(3)
A 		105
avr 12.5
(-1.6) 		9.6
(-2) 		3.3
(-3) 		42.6
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 3.8
(-1.6)
A 		3.3
(-2.2)
A 		3.5
(-1.9)
A 		4.1
(-1.2)
A 		2.6
(-3)
A 		7.6
(3)
A 		5
B 1.9
(-3)
A 		8
(3)
A 		2.3
(-2.6)
A 		2.1
(-2.8)
A 		1.9
(-3)
A 		2
(-2.9)
A 		6
C 3.1
(-2.7)
A 		14.6
(1.2)
A 		4.1
(-2.4)
A 		19.8
(3)
A 		2.2
(-3)
A 		2.6
(-2.9)
A 		18
avr 2.9
(-2.4) 		8.6
(2.9) 		3.3
(-2) 		8.7
(3) 		2.2
(-3) 		4.1
(-1.2) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 1.2
(-1.6)
A 		2.4
(2.6)
A 		0.8
(-3)
A 		2.5
(3)
A 		2
B 9.7
(3)
A 		0.8
(-2.8)
A 		0.8
(-2.8)
A 		0.5
(-3)
A 		9
C 1.2
(-3)
A 		4.1
(-2)
A 		1.3
(-3)
A 		18.3
(3)
A 		17
avr 4
(-0) 		2.4
(-1.6) 		1
(-3) 		7.1
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 0.7
(-3)
A 		1.2
(-0.5)
A 		0.8
(-2.5)
A 		1
(-1.5)
A 		0.8
(-2.5)
A 		1.9
(3)
A 		1
B 0.7
(-2.4)
A 		2.6
(3)
A 		0.5
(-3)
A 		0.6
(-2.7)
A 		0.8
(-2.1)
A 		0.8
(-2.1)
A 		2
C 1.2
(-2.2)
A 		4.5
(3)
A 		1
(-2.5)
A 		4
(2.2)
A 		0.7
(-3)
A 		0.9
(-2.7)
A 		4
avr 0.9
(-2.7) 		2.8
(3) 		0.8
(-3) 		1.9
(0.3) 		0.8
(-3) 		1.2
(-1.8) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176335_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4953
Sequence Type Exemplar sequence
Representative Public ID F28A10.9 NCBI
Target Description F28A10.9 /REP_DB=WormBase Gene ID /WP=CE19414 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061426(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061426 NCBI Caenorhabditis elegans F28A10.9 (F28A10.9) mRNA, complete cds. 11/11 None
SNAP00000009509 ENSEMBL cdna:SNAP chromosome:CEL140:II:833346:834445:-1 10/11 None
GENEFINDER00000009524 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:833545:836909:-1 10/11 None
F28A10.9 ENSEMBL cdna:known chromosome:CEL140:II:833613:835276:-1 gene:F28A10.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:833612-835276(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28A10.9
Entrez Gene 185034 Entrez gene
WormBase CE39014 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85475 Hypothetical protein F28A10.9 [Caenorhabditis elegans] ref|NP_493827.1| F28A10.9 [Caenorhabditis elegans] 0.0
blast AAK85475 Hypothetical protein F28A10.9 [Caenorhabditis elegans] ref|NP_493827.1| F28A10.9 [Caenorhabditis elegans] 1.0E-168
blast AAB66184 Hypothetical protein T07D3.4 [Caenorhabditis elegans] ref|NP_493840.1| T07D3.4 [Caenorhabditis elegans] 1.0E-127
blast AAK85475 Hypothetical protein F28A10.9 [Caenorhabditis elegans] ref|NP_493827.1| F28A10.9 [Caenorhabditis elegans] 1.0E-111
blast AAB66184 Hypothetical protein T07D3.4 [Caenorhabditis elegans] ref|NP_493840.1| T07D3.4 [Caenorhabditis elegans] 1.0E-100
blast AAB66184 Hypothetical protein T07D3.4 [Caenorhabditis elegans] ref|NP_493840.1| T07D3.4 [Caenorhabditis elegans] 2.0E-84
blast AAA64323 Hypothetical protein W02B3.4 [Caenorhabditis elegans] ref|NP_497236.2| W02B3.4 [Caenorhabditis elegans] sp|Q09341|YR24_CAEEL Hypothetical protein W02B3.4 in chromosome III 5.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR009644 EMBL-EBI Fukutin-related 5.2E-17
Pfam IPR009644 EMBL-EBI Fukutin-related 4.1E-19
Pfam IPR009644 EMBL-EBI Fukutin-related 4.9E-20
Pfam IPR009644 EMBL-EBI Fukutin-related 3.8E-19
Pfam IPR009644 EMBL-EBI Fukutin-related 4.9E-20
Pfam IPR009644 EMBL-EBI Fukutin-related 4.8E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493827.1 1 9-26
F28A10.9 1 9-26
Sequence
Target Sequence 
>C. ELEGANS:176335_AT
acccatttctcaacggcggaacatttctcggctggtgccgtgagtgctctgtcattccgc
acaccaccgacatggatatatcgattttcgcaaacgagtacaatccagactatgtgaaat
tgctgcagagctactggaatccgtctagtcttgaagcttggcgaatgcttggattgattg
atgactccctggagattactatgcgtccaaaacaatggtccgagtacccaattgatttat
ttttgatgtatgatggaatggagattggaaccctcacacatcattgggttggaggacttg
gcactgatggtaggaaatacaagtacaactaccatatctacgatccatggagtgcggcgg
atcttcatgggcatattttctggttctcctgcactcctcttgagaaaattttggctgagt
atggagagaactggaacaaggatcacctgtcatccgaatacagatggaattattcgcaga
aaaatgtaaagaaaaatgggaaactcacagaggaagagatgaacacggtttatagatcac
ctactgttggtgttcgtgccgttcaaagagtttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCCATTTCTCAACGGCGGAACATT 426 89 533 Antisense
GGAATCCGTCTAGTCTTGAAGCTTG 663 531 668 Antisense
GACTCCCTGGAGATTACTATGCGTC 335 373 715 Antisense
ATGGAGATTGGAACCCTCACACATC 48 53 790 Antisense
TGGGTTGGAGGACTTGGCACTGATG 540 553 817 Antisense
GTACAACTACCATATCTACGATCCA 609 459 855 Antisense
TCCATGGAGTGCGGCGGATCTTCAT 145 591 876 Antisense
TTCTCCTGCACTCCTCTTGAGAAAA 611 693 916 Antisense
GGAACAAGGATCACCTGTCATCCGA 199 529 965 Antisense
GATCACCTACTGTTGGTGTTCGTGC 49 421 1067 Antisense
GTGTTCGTGCCGTTCAAAGAGTTTT 121 479 1082 Antisense
Sequence Source Affymetrix Proprietary Database