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[176334_at]

Description Y50D4B.5 /REP_DB=WormBase Gene ID /WP=CE22283 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 11      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 3.2
(-3)
A 		8.6
(-2.4)
A 		9.6
(-2.3)
A 		55.1
(3)
A 		52
B 5.4
(-2.1)
A 		12
(3)
A 		4.7
(-2.6)
A 		4.2
(-3)
A 		8
C 16
(0.1)
A 		12.6
(-0.7)
A 		3.3
(-3)
A 		27.8
(3)
A 		25
avr 8.2
(-2.4) 		11.1
(-1.6) 		5.9
(-3) 		29
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 3.1
(-3)
A 		6.2
(-0.9)
A 		3.3
(-2.9)
A 		7
(-0.3)
A 		9.1
(1.1)
A 		11.9
(3)
A 		9
B 16.2
(1.1)
A 		15.3
(0.7)
A 		14.6
(0.4)
A 		7.3
(-3)
A 		13.4
(-0.2)
A 		20.3
(3)
A 		13
C 5.9
(-2.8)
A 		5.9
(-2.8)
A 		24.7
(1.3)
A 		32.2
(3)
A 		9.6
(-2)
A 		5.1
(-3)
A 		27
avr 8.4
(-3) 		9.1
(-2.4) 		14.2
(1.9) 		15.5
(3) 		10.7
(-1.1) 		12.4
(0.4) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.2
(-3)
A 		1.9
(-2.4)
A 		2.5
(-1.9)
A 		8.1
(3)
A 		7
B 1.7
(-0.7)
A 		3
(3)
A 		1.1
(-2.4)
A 		0.9
(-3)
A 		2
C 5.3
(3)
A 		3.6
(0.6)
A 		1.1
(-3)
A 		4.7
(2.1)
A 		4
avr 2.7
(-0.8) 		2.8
(-0.6) 		1.6
(-3) 		4.6
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.6
(-3)
A 		2.2
(1.2)
A 		0.7
(-2.7)
A 		1.8
(0.1)
A 		2.8
(2.7)
A 		2.9
(3)
A 		2
B 5.6
(0.9)
A 		5.1
(0.4)
A 		3.5
(-1.3)
A 		1.9
(-3)
A 		5.4
(0.7)
A 		7.6
(3)
A 		6
C 2.2
(-2.4)
A 		1.8
(-2.9)
A 		6.3
(2.8)
A 		6.5
(3)
A 		3.1
(-1.3)
A 		1.7
(-3)
A 		5
avr 2.8
(-3) 		3
(-2.1) 		3.5
(0.2) 		3.4
(-0.2) 		3.8
(1.6) 		4.1
(3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176334_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15327
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.5 NCBI
Target Description Y50D4B.5 /REP_DB=WormBase Gene ID /WP=CE22283 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070975(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070975 NCBI Caenorhabditis elegans Y50D4B.5 (Y50D4B.5) mRNA, complete cds. 11/11 None
SNAP00000024548 ENSEMBL cdna:SNAP chromosome:CEL140:V:1089121:1089721:1 10/11 None
GENEFINDER00000024552 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1088199:1103876:1 10/11 None
Y50D4B.5 ENSEMBL cdna:known chromosome:CEL140:V:1088199:1089721:1 gene:Y50D4B.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024547 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1088198-1089721(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.5
Entrez Gene 190095 Entrez gene
SwissProt Q9N4T1 EMBL-EBI
WormBase CE22283 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5529 sugar binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF59494 Hypothetical protein Y50D4B.5 [Caenorhabditis elegans] ref|NP_503376.1| Y50D4B.5 [Caenorhabditis elegans] 0.0
blast AAK84612 Hypothetical protein Y50D4B.4 [Caenorhabditis elegans] ref|NP_503377.2| Y50D4B.4 [Caenorhabditis elegans] 0.0
blast AAF59494 Hypothetical protein Y50D4B.5 [Caenorhabditis elegans] ref|NP_503376.1| Y50D4B.5 [Caenorhabditis elegans] 1.0E-174
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 1.0E-168
blast AAF59494 Hypothetical protein Y50D4B.5 [Caenorhabditis elegans] ref|NP_503376.1| Y50D4B.5 [Caenorhabditis elegans] 2.0E-83
blast CAB02971 Hypothetical protein F17B5.3 [Caenorhabditis elegans] ref|NP_493310.1| F17B5.3 [Caenorhabditis elegans] 5.0E-83
blast CAB02971 Hypothetical protein F17B5.3 [Caenorhabditis elegans] ref|NP_493310.1| F17B5.3 [Caenorhabditis elegans] 8.0E-69
blast CAA22313 Hypothetical protein Y18D10A.10 [Caenorhabditis elegans] ref|NP_493248.1| Y18D10A.10 [Caenorhabditis elegans] 5.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.1E-31
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503376.1 2 52-74,84-106
Y50D4B.5 2 52-74,84-106
GENEFINDER00000024552 3 38-60,70-92,402-424
Sequence
Target Sequence 
>C. ELEGANS:176334_AT
taacagcattgccagtttccggagaaagacccatatctacgattattgtcaatcatggag
agaatcatgaaaatccacctctcgctcaagttgccacgtgtccagatgattggatgactt
atgaaagaccacaaggaagatggtgtatgaaggcgttctacggaaagatgtcccagtccg
atgccgaagcagagtgcaacgcagtcggagcaaaactttcaggacttcaaaacgcaaacg
aaaggatgaatatttcttatgttcttcgtgacttggtatacaaggatggtggtggaaaat
acacagcttggctcggtgggaagagaaaagctagctgcccgaccggcgcctcttgtgccc
gactgaacagtatcgagtggactgatgggcacacgacaggcactgatggattttcaactg
gtactcgggagctcgacggcacttatctacaaaaattccggggagttcaacaatgtttgc
acatgattgtcactccatattccgataccgagcttggattcgcagacttcgttcacggtt
cagtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAACAGCATTGCCAGTTTCCGGAGA 666 639 515 Antisense
CCCAGTCCGATGCCGAAGCAGAGTG 342 257 686 Antisense
TTATGTTCTTCGTGACTTGGTATAC 245 679 771 Antisense
AATACACAGCTTGGCTCGGTGGGAA 710 169 812 Antisense
GAAGAGAAAAGCTAGCTGCCCGACC 246 339 834 Antisense
GGACTGATGGGCACACGACAGGCAC 610 523 893 Antisense
TTTTCAACTGGTACTCGGGAGCTCG 421 667 925 Antisense
GCTCGACGGCACTTATCTACAAAAA 518 297 945 Antisense
ACTCCATATTCCGATACCGAGCTTG 448 97 1006 Antisense
GAGCTTGGATTCGCAGACTTCGTTC 418 389 1024 Antisense
GCAGACTTCGTTCACGGTTCAGTGG 463 303 1036 Antisense
Sequence Source Affymetrix Proprietary Database