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[176332_s_at]

Description Y50D4B.4 /REP_DB=WormBase Gene ID /WP=CE26135 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 12      4grp_high_8_8 > 58      6grp_highest_nonorm > 61      6grp_total > 23      4grp_total > 61      6grp_highest_sub > 01_02_03      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 242
1 2 3 4 range
A 291
(3)
P 		173
(0.1)
P 		187.7
(0.4)
P 		48.8
(-3)
A 		242
B 153.1
(2.8)
P 		127.9
(1)
P 		155.8
(3)
P 		73
(-3)
A 		83
C 260.4
(3)
P 		193
(1)
P 		253.7
(2.8)
P 		61.6
(-3)
P 		199
avr 234.8
(3) 		164.6
(0.6) 		199.1
(1.8) 		61.1
(-3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 788
1 2 3 4 5 6 range
A 781.1
(3)
P 		704.3
(2.3)
P 		458.3
(0.2)
P 		330.5
(-1)
P 		251.5
(-1.7)
P 		100
(-3)
P 		681
B 447.8
(3)
P 		424.3
(2.6)
P 		443.3
(2.9)
P 		305.3
(0.7)
P 		173.8
(-1.4)
P 		77.6
(-3)
P 		370
C 536.7
(0.5)
P 		570
(0.7)
P 		865.7
(3)
P 		374.6
(-0.8)
P 		173.8
(-2.4)
P 		90.5
(-3)
P 		775
avr 588.5
(3) 		566.2
(2.7) 		589.1
(3) 		336.8
(-0) 		199.7
(-1.7) 		89.4
(-3) 		500
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 105.9
(3)
P 		37.6
(-1.1)
P 		49.1
(-0.4)
P 		7.1
(-3)
A 		99
B 48.8
(3)
P 		31.6
(-0.1)
P 		36.1
(0.7)
P 		15.6
(-3)
A 		33
C 86.2
(2.8)
P 		55.5
(0.5)
P 		88.4
(3)
P 		10.4
(-3)
P 		78
avr 80.3
(3) 		41.6
(-0.4) 		57.9
(1.1) 		11
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 226
1 2 3 4 5 6 range
A 144
(0.2)
P 		250.5
(3)
P 		99.8
(-1)
P 		83.8
(-1.4)
P 		75.9
(-1.6)
P 		24.4
(-3)
P 		226
B 155.8
(3)
P 		141.2
(2.3)
P 		107.6
(0.7)
P 		80.9
(-0.5)
P 		70.6
(-1)
P 		28.9
(-3)
P 		127
C 198.9
(2.3)
P 		173.9
(1.5)
P 		220.3
(3)
P 		75.3
(-1.6)
P 		56.2
(-2.2)
P 		30.3
(-3)
P 		190
avr 166.2
(2.2) 		188.5
(3) 		142.6
(1.3) 		80
(-1.1) 		67.6
(-1.5) 		27.9
(-3) 		161
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176332_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15441
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.4 NCBI
Target Description Y50D4B.4 /REP_DB=WormBase Gene ID /WP=CE26135 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070977(11), NM_070976(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070977 NCBI Caenorhabditis elegans Y50D4B.3 (Y50D4B.3) mRNA, complete cds. 11/11 A 
NM_070976 NCBI Caenorhabditis elegans Y50D4B.4 (Y50D4B.4) mRNA, complete cds. 11/11 A 
SNAP00000024549 ENSEMBL cdna:SNAP chromosome:CEL140:V:1091731:1100545:1 11/11 None
GENEFINDER00000024552 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1088199:1103876:1 11/11 None
Y50D4B.4 ENSEMBL cdna:known chromosome:CEL140:V:1092509:1094946:1 gene:Y50D4B.4 11/11 A 
Y50D4B.3 ENSEMBL cdna:known chromosome:CEL140:V:1095192:1098451:1 gene:Y50D4B.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1092508-1094946(+) 98.25 100.0
Public Domain and Genome References
Gene Title

Ensembl Y50D4B.4
Y50D4B.3
Entrez Gene 178608 Entrez gene
3565176 Entrez gene
SwissProt Q966B0 EMBL-EBI
Q966B1 EMBL-EBI
WormBase CE26134 Wormbase
CE35918 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 0.0
blast AAK84612 Hypothetical protein Y50D4B.4 [Caenorhabditis elegans] ref|NP_503377.2| Y50D4B.4 [Caenorhabditis elegans] 0.0
blast AAF59494 Hypothetical protein Y50D4B.5 [Caenorhabditis elegans] ref|NP_503376.1| Y50D4B.5 [Caenorhabditis elegans] 1.0E-174
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 1.0E-168
blast CAB03891 Hypothetical protein C17D12.3 [Caenorhabditis elegans] ref|NP_492962.1| C17D12.3 [Caenorhabditis elegans] 1.0E-157
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.1E-31
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 8.1E-24
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.1E-31
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503377.2 1 30-52
Y50D4B.4 1 30-52
GENEFINDER00000024552 3 38-60,70-92,402-424
Sequence
Target Sequence 
>C. ELEGANS:176332_S_AT
gggtttcaacgcaagatcaccttctcaacatttcctctacattccatgacgacagtggac
gggtttttgcactgtccccaaagactggagaaaactatgcaaagttcacgtataacttcc
ttaataattcgtattcacacaacgagccagatgggtcaccagtcagattattcgatttta
atggcttggaacgagtagaggattatacagagaatactgtattcttacgcagaaaaaacc
caaaggaaaagggaaacattaaatgggcgctcgatttgaatatcgatggactatcagtgg
agacagttgtgatcaaaatcataggaatcgacgaaattgacaatggtggaggaacggcca
tagcctgtggacatgatcttacttcaatagttaacccgagatgcggcaatattccgttta
acggaaaaatcactttttttaataacgctttccatcgtttatatttgaatataacgctgg
acgaggatattcaaatttttaagaccaaactcgatgacgagggcagtttggagaatgttt
ctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTTTCAACGCAAGATCACCTTCT 319 497 752 Antisense
TCAACGCAAGATCACCTTCTCAACA 534 631 757 Antisense
TCAACATTTCCTCTACATTCCATGA 75 631 776 Antisense
TGACGACAGTGGACGGGTTTTTGCA 107 565 798 Antisense
TTGCACTGTCCCCAAAGACTGGAGA 214 703 818 Antisense
CGAGCCAGATGGGTCACCAGTCAGA 116 251 894 Antisense
GGGTCACCAGTCAGATTATTCGATT 294 497 904 Antisense
GATTATTCGATTTTAATGGCTTGGA 557 427 917 Antisense
ATGGCTTGGAACGAGTAGAGGATTA 534 49 932 Antisense
GAATACTGTATTCTTACGCAGAAAA 386 327 963 Antisense
GAGGGCAGTTTGGAGAATGTTTCTG 353 401 1270 Antisense
Sequence Source Affymetrix Proprietary Database