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[176331_at]

Description Y50D4B.4 /REP_DB=WormBase Gene ID /WP=CE26135 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 32      4grp_total > 52      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 56.5
(0)
P 		68.5
(3)
P 		47.7
(-2.2)
P 		44.4
(-3)
A 		24
B 63.9
(3)
P 		48.5
(1.5)
M 		49.8
(1.7)
A 		0.9
(-3)
A 		63
C 56.9
(-0)
P 		83.9
(3)
P 		44.9
(-1.3)
P 		29.9
(-3)
A 		54
avr 59.1
(1.9) 		67
(3) 		47.5
(0.2) 		25.1
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 79.2
(-0.4)
P 		100.2
(0.9)
P 		134.4
(3)
P 		104.5
(1.2)
P 		63.5
(-1.3)
P 		35.6
(-3)
A 		99
B 95.5
(1.2)
P 		108.9
(1.9)
P 		130.8
(3)
P 		69.5
(-0.1)
P 		38.5
(-1.7)
A 		11.9
(-3)
A 		119
C 138.9
(2.9)
P 		86.3
(0)
P 		111.8
(1.4)
P 		141.6
(3)
P 		66.1
(-1.1)
P 		30
(-3)
A 		112
avr 104.5
(1.7) 		98.5
(1.4) 		125.7
(3) 		105.2
(1.8) 		56
(-1.2) 		25.8
(-3) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 20.6
(3)
P 		14.9
(0.6)
P 		12.5
(-0.4)
P 		6.5
(-3)
A 		14
B 20.4
(3)
P 		12
(0.5)
M 		11.5
(0.4)
A 		0.2
(-3)
A 		20
C 18.8
(1.3)
P 		24.1
(3)
P 		15.7
(0.4)
P 		5
(-3)
A 		19
avr 19.9
(3) 		17
(1.9) 		13.2
(0.5) 		3.9
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 14.6
(-1.7)
P 		35.7
(3)
P 		29.3
(1.6)
P 		26.5
(1)
P 		19.2
(-0.7)
P 		8.7
(-3)
A 		27
B 33.2
(2.4)
P 		36.2
(3)
P 		31.8
(2.2)
P 		18.4
(-0.4)
P 		15.7
(-0.9)
A 		4.4
(-3)
A 		32
C 51.5
(3)
P 		26.3
(-0.6)
P 		28.5
(-0.3)
P 		28.5
(-0.3)
P 		21.4
(-1.4)
P 		10
(-3)
A 		42
avr 33.1
(3) 		32.7
(2.9) 		29.9
(2.2) 		24.5
(1) 		18.8
(-0.4) 		7.7
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176331_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15441
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.4 NCBI
Target Description Y50D4B.4 /REP_DB=WormBase Gene ID /WP=CE26135 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070976(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070976 NCBI Caenorhabditis elegans Y50D4B.4 (Y50D4B.4) mRNA, complete cds. 11/11 A 
GENEFINDER00000024552 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1088199:1103876:1 11/11 None
Y50D4B.4 ENSEMBL cdna:known chromosome:CEL140:V:1092509:1094946:1 gene:Y50D4B.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070977 1/11 Cross Hyb Matching Probes A 
SNAP00000024549 8/11 Cross Hyb Matching Probes None
Y50D4B.3 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1092508-1094946(+) 98.25 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.4
Entrez Gene 3565176 Entrez gene
SwissProt Q966B1 EMBL-EBI
WormBase CE35918 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84612 Hypothetical protein Y50D4B.4 [Caenorhabditis elegans] ref|NP_503377.2| Y50D4B.4 [Caenorhabditis elegans] 0.0
blast AAF59494 Hypothetical protein Y50D4B.5 [Caenorhabditis elegans] ref|NP_503376.1| Y50D4B.5 [Caenorhabditis elegans] 1.0E-174
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 1.0E-168
blast CAB03891 Hypothetical protein C17D12.3 [Caenorhabditis elegans] ref|NP_492962.1| C17D12.3 [Caenorhabditis elegans] 1.0E-157
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.1E-31
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.1E-31
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503377.2 1 30-52
Y50D4B.4 1 30-52
GENEFINDER00000024552 3 38-60,70-92,402-424
Sequence
Target Sequence 
>C. ELEGANS:176331_AT
atgggatgtgaggagcttgagagcggtttttcggttaaagcctactgggtttcaacgcaa
gatcaccttctcaacatttcctctacattccatgacgacagtggacgggtttttgcactg
tccccaaagactggagaaaactatgcaaagttcacgtataacttccttaataattcgtat
tcacacaacgagccagatgggtcaccagtcagattattcgattttaatggcttggaacga
gtagaggattatacagagaatactgtattcttacgcagaaaaaacccaaaggaaaaggga
aacattaaatgggcgctcgatttgaatatcgatggactatcagtggagacagttgtgatc
aaaatcataggaatcgacgaaattgacaatggtggaggaacggccatagcctgtggacat
gatcttacttcaatagttaacccgagatgcggcaatattccgtttaacggaaaaatcact
ttttttaataacgctttccatcgtttatatttgaatataacgctggacgaggatattcaa
atttttaagaccaaactcgatgacgagggcagtttggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGGATGTGAGGAGCTTGAGAGCG 122 51 706 Antisense
TAAAGCCTACTGGGTTTCAACGCAA 263 641 741 Antisense
ATTCGTATTCACACAACGAGCCAGA 538 9 878 Antisense
GAAACATTAAATGGGCGCTCGATTT 34 353 1004 Antisense
ATCGATGGACTATCAGTGGAGACAG 535 35 1033 Antisense
GGAACGGCCATAGCCTGTGGACATG 379 529 1102 Antisense
GCCTGTGGACATGATCTTACTTCAA 6 283 1114 Antisense
ACTTCAATAGTTAACCCGAGATGCG 597 99 1132 Antisense
GATGCGGCAATATTCCGTTTAACGG 331 413 1151 Antisense
TAATAACGCTTTCCATCGTTTATAT 621 635 1191 Antisense
ACTCGATGACGAGGGCAGTTTGGAG 386 97 1260 Antisense
Sequence Source Affymetrix Proprietary Database