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[176317_at]

Description F28A10.4 /REP_DB=WormBase Gene ID /WP=CE19409 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 10      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 4.4
(-3)
A 		4.6
(-3)
A 		5.7
(-2.8)
A 		40.8
(3)
A 		36
B 4.9
(-3)
A 		13
(-0.5)
A 		9.3
(-1.6)
A 		24.2
(3)
A 		19
C 7.7
(-2.6)
A 		5.3
(-2.7)
A 		1.8
(-3)
A 		81.7
(3)
A 		80
avr 5.7
(-3) 		7.6
(-2.7) 		5.6
(-3) 		48.9
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 4.8
(-3)
A 		6.1
(-2.9)
A 		20.5
(-1.2)
A 		5.7
(-2.9)
A 		58
(3)
A 		38.1
(0.8)
A 		53
B 4.5
(-2.7)
A 		6.1
(-2.4)
A 		2
(-3)
A 		45.1
(3)
A 		11.5
(-1.7)
A 		4.7
(-2.6)
A 		43
C 2.1
(-3)
A 		7.7
(-2.3)
A 		14.9
(-1.4)
A 		41.5
(1.9)
A 		50.7
(3)
A 		4.2
(-2.7)
A 		49
avr 3.8
(-3) 		6.6
(-2.5) 		12.5
(-1.6) 		30.8
(1.5) 		40.1
(3) 		15.7
(-1) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 1.6
(-2.3)
A 		1
(-3)
A 		1.5
(-2.4)
A 		6
(3)
A 		5
B 1.6
(-3)
A 		3.2
(-0.3)
A 		2.1
(-2.2)
A 		5.2
(3)
A 		4
C 2.6
(-2.1)
A 		1.5
(-2.6)
A 		0.6
(-3)
A 		13.8
(3)
A 		13
avr 1.9
(-2.6) 		1.9
(-2.6) 		1.4
(-3) 		8.3
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 0.9
(-3)
A 		2.2
(-2.5)
A 		4.5
(-1.7)
A 		1.4
(-2.8)
A 		17.5
(3)
A 		9.3
(0)
A 		17
B 1.6
(-2.4)
A 		2
(-2.2)
A 		0.5
(-3)
A 		11.9
(3)
A 		4.7
(-0.8)
A 		1.7
(-2.4)
A 		11
C 0.8
(-3)
A 		2.3
(-2.4)
A 		3.8
(-1.8)
A 		8.3
(-0.1)
A 		16.4
(3)
A 		1.4
(-2.8)
A 		16
avr 1.1
(-3) 		2.2
(-2.4) 		2.9
(-2.1) 		7.2
(0.1) 		12.9
(3) 		4.1
(-1.5) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176317_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5513
Sequence Type Exemplar sequence
Representative Public ID F28A10.4 NCBI
Target Description F28A10.4 /REP_DB=WormBase Gene ID /WP=CE19409 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061429(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061429 NCBI Caenorhabditis elegans F28A10.4 (F28A10.4) mRNA, complete cds. 11/11 None
SNAP00000009512 ENSEMBL cdna:SNAP chromosome:CEL140:II:844280:845091:1 11/11 None
GENEFINDER00000009527 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:844280:845091:1 11/11 None
F28A10.4 ENSEMBL cdna:known chromosome:CEL140:II:844280:845091:1 gene:F28A10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:844279-845091(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28A10.4
Entrez Gene 185030 Entrez gene
SwissProt Q95YB4 EMBL-EBI
WormBase CE19409 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85470 Hypothetical protein F28A10.4 [Caenorhabditis elegans] ref|NP_493830.1| F28A10.4 [Caenorhabditis elegans] 1.0E-128
blast AAP13767 Hypothetical protein T23E1.3 [Caenorhabditis elegans] ref|NP_001023400.1| T23E1.3 [Caenorhabditis elegans] 1.0E-83
blast CAE59891 Hypothetical protein CBG03375 [Caenorhabditis briggsae] 2.0E-44
Sequence
Target Sequence 
>C. ELEGANS:176317_AT
gaagctggaatacatgtgcatcgatcattaaaccaacttctcaatctgaagattactatg
gagatatgcaactggtttggattgaagcaactcttggaaaagtatttggattctgaggat
ttttctcaaactgcgatggttttattgcatagaattcgagttttggagatgaagccgacc
gaccctcgaaagcgctttgcatcggagtcttattcttctcaaaaaatatcgaaacgttca
ccgattccgtatagaaaagcagtaactcttcagacagactggataatagagcccccatca
gttcctgaaaaagttcctgaattgattgacgtttctgaacttcaaaatctggaacaattt
gagtacgaaagactgcgcccaacacttataactctcacagcaaaacggttgaaatcgttc
atggatcgtaatccggaaattgaaaaaatcgccgatgggctgttcaggcggcattgccag
gttgattgcccggaagacatgaagtacaagacaagcacagaaacatggagaactatacac
gatgtgcatattaactggaaaatgtatgaagaggctttggcacgggatgacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCTGGAATACATGTGCATCGAT 445 343 64 Antisense
GCATCGATCATTAAACCAACTTCTC 613 307 81 Antisense
CCCTCGAAAGCGCTTTGCATCGGAG 628 259 246 Antisense
TGCATCGGAGTCTTATTCTTCTCAA 133 579 261 Antisense
CTGGATAATAGAGCCCCCATCAGTT 181 239 342 Antisense
TGAGTACGAAAGACTGCGCCCAACA 36 569 423 Antisense
GCCCAACACTTATAACTCTCACAGC 582 285 440 Antisense
AAAATCGCCGATGGGCTGTTCAGGC 309 133 508 Antisense
GCTGTTCAGGCGGCATTGCCAGGTT 449 293 522 Antisense
TTGCCAGGTTGATTGCCCGGAAGAC 580 701 537 Antisense
AAGAGGCTTTGGCACGGGATGACGT 186 151 632 Antisense
Sequence Source Affymetrix Proprietary Database