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[176315_at]

Description Y105E8B.DD /REP_DB=WormBase Gene ID /WP=CE25705 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_high1_10_10 > 55      4grp_highest_8_8 > 43      4grp_high_8_8 > 56      6grp_total > 56      4grp_total > 55      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 123
1 2 3 4 range
A 362.1
(3)
P 		266.7
(-3)
P 		344.6
(1.9)
P 		302.2
(-0.8)
P 		95
B 313.5
(-0.4)
P 		289.4
(-3)
P 		345.3
(3)
P 		309.6
(-0.8)
P 		56
C 382.4
(3)
P 		271.3
(-2.4)
P 		259.2
(-3)
P 		304
(-0.8)
P 		123
avr 352.7
(3) 		275.8
(-3) 		316.4
(0.2) 		305.3
(-0.7) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 208
1 2 3 4 5 6 range
A 65.2
(-3)
A 		176.9
(1.6)
P 		111.3
(-1.1)
P 		121
(-0.7)
P 		109.3
(-1.2)
P 		212.1
(3)
P 		147
B 136.4
(-0.5)
P 		173.5
(0.6)
P 		127.7
(-0.8)
P 		95.9
(-1.7)
P 		50.3
(-3)
A 		258
(3)
P 		208
C 106.3
(-1.6)
P 		206.9
(1.8)
P 		243.4
(3)
P 		171.9
(0.6)
P 		62.8
(-3)
P 		241.5
(2.9)
P 		181
avr 102.6
(-2) 		185.8
(1.1) 		160.8
(0.2) 		129.6
(-1) 		74.1
(-3) 		237.2
(3) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 131.8
(3)
P 		57.9
(-2.1)
P 		90.1
(0.1)
P 		44.2
(-3)
P 		88
B 100
(3)
P 		71.5
(-2)
P 		80
(-0.5)
P 		66
(-3)
P 		34
C 126.6
(3)
P 		78
(-0.9)
P 		90.3
(0.1)
P 		51.3
(-3)
P 		75
avr 119.5
(3) 		69.1
(-1.6) 		86.8
(0) 		53.8
(-3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 12
(-3)
A 		62.9
(3)
P 		24.2
(-1.6)
P 		30.7
(-0.8)
P 		33
(-0.5)
P 		51.7
(1.7)
P 		51
B 47.5
(-0.9)
P 		57.7
(-0)
P 		31
(-2.2)
P 		25.4
(-2.6)
P 		20.4
(-3)
A 		96.2
(3)
P 		76
C 39.4
(-1.1)
P 		63.1
(1.2)
P 		61.9
(1.1)
P 		34.6
(-1.6)
P 		20.3
(-3)
P 		80.8
(3)
P 		61
avr 33
(-2) 		61.2
(1.3) 		39
(-1.3) 		30.2
(-2.3) 		24.6
(-3) 		76.2
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176315_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18766
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.DD NCBI
Target Description Y105E8B.DD /REP_DB=WormBase Gene ID /WP=CE25705 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170957(10), NM_001026518(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170957 NCBI Caenorhabditis elegans Y105E8A.25 (Y105E8A.25) mRNA, complete cds. 10/11 A 
NM_001026518 NCBI Caenorhabditis elegans Y105E8A.29 (Y105E8A.29) mRNA, complete cds. 10/11 None
SNAP00000013363 ENSEMBL cdna:SNAP chromosome:CEL140:I:14594551:14610260:-1 10/11 None
GENEFINDER00000013507 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14600186:14612257:-1 11/11 None
Y105E8A.29 ENSEMBL cdna:known chromosome:CEL140:I:14593530:14608407:-1 gene:Y105E8A.29 10/11 A 
Y105E8A.25 ENSEMBL cdna:known chromosome:CEL140:I:14600061:14612257:-1 gene:Y105E8A.25 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14600116-14604674(-) 94.39 100.0
Public Domain and Genome References
Gene Title

Ensembl Y105E8A.29
Y105E8A.25
Entrez Gene 3564893 Entrez gene
3565958 Entrez gene
SwissProt Q8WQA2 EMBL-EBI
Q7YTJ0 EMBL-EBI
WormBase CE29843 Wormbase
CE35056 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21671 Hypothetical protein Y105E8A.25 [Caenorhabditis elegans] ref|NP_740953.1| Y105E8A.25 [Caenorhabditis elegans] 0.0
blast CAE17987 Hypothetical protein Y105E8A.29 [Caenorhabditis elegans] ref|NP_001021689.1| Y105E8A.29 [Caenorhabditis elegans] 1.0E-145
blast CAD21671 Hypothetical protein Y105E8A.25 [Caenorhabditis elegans] ref|NP_740953.1| Y105E8A.25 [Caenorhabditis elegans] 1.0E-137
blast CAE17987 Hypothetical protein Y105E8A.29 [Caenorhabditis elegans] ref|NP_001021689.1| Y105E8A.29 [Caenorhabditis elegans] 4.0E-97
blast CAE72602 Hypothetical protein CBG19792 [Caenorhabditis briggsae] 7.0E-97
blast CAE72602 Hypothetical protein CBG19792 [Caenorhabditis briggsae] 7.0E-92
Sequence
Target Sequence 
>C. ELEGANS:176315_AT
tgatgggggtgcagtactctattcgagaatatacgttcgaaaagcgttcggcatctacaa
atcacttgagcaaaagccgaaaaggtgctacattggtattccataatgatccaaatgaaa
aagacgaaacagatgatgaagaggaatatgaagatgctgcagatggatattcaaaagaca
atgaaatgctcattttggagaaatctggtggagaagaacgatatcgaatggcttctgtga
agaagaagaatggttctccattacgtcattttgacaatcactctaacctgacatcgattt
catctacggaaccaccgccagttcgattgcccagaagagtatcagcagtatcttcaacat
cacgttcatcacctcaaagagctacggatcagtcgtggaaggataaaatgccaccattcg
gaattgttgggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGGGGGTGCAGTACTCTATTCG 52 557 476 Antisense
GCGTTCGGCATCTACAAATCACTTG 398 291 519 Antisense
AAAGGTGCTACATTGGTATTCCATA 235 121 556 Antisense
GAACGATATCGAATGGCTTCTGTGA 390 347 691 Antisense
GGTTCTCCATTACGTCATTTTGACA 285 507 727 Antisense
GACAATCACTCTAACCTGACATCGA 282 365 748 Antisense
GACATCGATTTCATCTACGGAACCA 388 369 765 Antisense
GCCAGTTCGATTGCCCAGAAGAGTA 105 281 792 Antisense
TCAGCAGTATCTTCAACATCACGTT 387 625 817 Antisense
ATCACGTTCATCACCTCAAAGAGCT 50 29 834 Antisense
ATGCCACCATTCGGAATTGTTGGGC 240 41 883 Antisense
Sequence Source Affymetrix Proprietary Database